Cloning and Characterization of 1,8-Cineole Synthase (SgCINS) Gene From the Leaves of Salvia guaranitica Plant

Abstract

Monoterpenes are one of the most common groups belonging to the terpenoid family, with a C10 structure comprising of two isoprene units. Most of monoterpenes are volatile plant compounds, and they act as signaling molecules between plants and the environment, particularly as defensive compounds against herbivores and pathogens. In this study, 1,8-cineole synthase (SgCINS) gene was identified and cloned from the leaves of Salvia guaranitica plant. To examine the role of SgCINS in insect resistance, we transformed and expressed this gene into tobacco leaves. The metabolic analysis revealed that the production of various types and amount of terpenoid was increased and decreased in SgCINS overexpression and control lines, respectively, suggesting that overexpressing SgCINS in transgenic tobacco plants lead to an increase in the production of various types of terpenoids and other phytochemical compounds. These results indicated why transgenic tobacco was highly resistant against cotton worm than the highly susceptible control plants. Our results demonstrate that the SgCINS gene can play an important role in plants against cotton worm insect attack, and pave the way for using terpenoids genes for improving resistance to insect attack in higher plants.

Biochemical basis for the formation of organ-specific volatile blends in mint

“…Moldenke (Despinasse et al, 2017;Hurd et al, 2017) (indicated as BPPS in Figure 4). Candidate Mp_12413 from peppermint clustered with 1,8-cineole synthases (labeled as CINS in Figure 4) of several species of the Lamiaceae (Wise et al, 1998;Kampranis et al, 2007;Demissie et al, 2012;Ali et al, 2022).…”

CLONING, MOLECULAR CHARACTERIZATION AND FUNCTIONAL ANALYSIS OF THE CIS-MUUROLADIENE SYNTHASE (SgCMS) GENE FROM LEAVES OF SALVIA GUARANITICA PLANT

“…For a rapid survey of terpenoids in transgenic and non-transgenic A. thaliana plant lines, intact leaves of various lines were frozen in liquid nitrogen (LN2), powdered with a ceramic mortar and pestle, and directly inundation in n-hexane as a solvent for 75 h for ensuring complete removal of terpenoids contents as previously described (Ali et al, 2022a;. Approximately 1 µl aliquot of each extract was analysed by the Shimadzu-GC-MS system with three replicates.…”

“…This magnesium cluster binds and interacts with the diphosphate moiety of farnesyl diphosphate (FPP), hence, catalyzing the C15-substrate-FPP formation at the hydrophobic substrate binding pocket (Su-Fang et al, 2014;Abbas et al 2019 andRebecca et al 2020). Eventually, each protein sequence belonging to the terpene synthase family has one or two of these conserved domains (Ali et al, 2017(Ali et al, , 2018(Ali et al, and 2022a. A phylogenetic tree was generated to analyse the evolutionary relationship between SgCMS and other plant sesquiterpene synthase genes, and the evolutionary tree was constructed well by the neighbor-joining method parameters.…”

Metabolic engineering of a 1,8‐cineole synthase enhances aphid repellence and increases trichome density in transgenic tobacco (Nicotiana tabacum L.)

“…29 Previous studies have demonstrated that transgenic tobacco overexpressing Salvia officinalis 1,8-cineole synthase (SoCINS) enhanced terpenoid production, 30 whereas ectopic expression of Salvia guaranitica (Sg)CINS in transgenic plants can improve resistance against cotton leafworms. 31 Moreover, SoCINS has been found to participate in the regulation of the number and fresh weight of nodules in soybean, suggesting that CINS plays a role in plant development. 32 In the present study, we observed that overexpression of SoCINS in tobacco plants resulted in increased emission of 1,8-cineole and trichome density, leading to a repellent effect on aphids.…”

“…1,8‐cineole synthase, a typical multiproduct enzyme in the plant kingdom, is capable of synthesizing the characteristic ‘cineole cassette’ monoterpenes, with 1,8‐cineole as the dominant volatile product in some Nicotiana species 29 . Previous studies have demonstrated that transgenic tobacco overexpressing Salvia officinalis 1,8‐cineole synthase ( SoCINS ) enhanced terpenoid production, 30 whereas ectopic expression of Salvia guaranitica ( Sg ) CINS in transgenic plants can improve resistance against cotton leafworms 31 . Moreover, SoCINS has been found to participate in the regulation of the number and fresh weight of nodules in soybean, suggesting that CINS plays a role in plant development 32 …”