Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp

Liu, F; Cao, Yanhui

doi:10.4238/2015.december.29.33

Cited by 5 publications

(3 citation statements)

References 23 publications

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“…The application of EPSPS gene in the transgenic glyphosate resistant crops is mainly used through overproduction of the wild-type EPSPS or expression of mutant EPSPS with glyphosate resistance [4][5][6][7]. Since the EPSPS gene plays an important role in increasing herbicide resistance, much studies have been…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca

Liu

Ruan

2021

Braz. arch. biol. technol.

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To discover and isolate a glyphosate-resistant gene from Fragaria vesca through gene mining. An open reading frame (ORF) of 1563 bp encoding EPSPSwas amplified from Fragaria vesca (FvEPSPS). FvEPSPS (Genebank: XP004306932.1) encodes a polypeptide of 520 amino acids and it has hightly homologous with EPSPS from other plants. qRT-PCR analysis showed that the FvEPSPS was expressed extensively in all tissues including leaves, roots and stems, with higher expression in leaves. Furthermore, transgenic Arabidopsis Thaliana exhibited 10 mM glyphosate to resistance. Therefore, this research offers a new glyphosate-resistant gene for development of transgenic crops.

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Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca

Liu

Ruan

2021

Braz. arch. biol. technol.

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“…have high amino acid homology, and their structural features are also quite similar [23]. In E. coli, the structure basis for the glyphosate insensitivity of TIPS EPSPS depends on changes in its spatial structure, especially the spatial orientation of Gly96.…”

Section: Introductionmentioning

confidence: 99%

Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Pantoea sp. 5-Enolpyruvylshikimate-3- Phosphate Synthase

Liu

Cao

2018

Journal of Microbiology and Biotechnology

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Glyphosate inhibits the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) in the shikimate pathway. A mutant of EPSPS from Pantoea sp. was identified using sitedirected mutagenesis. The mutant showed significantly improved glyphosate resistance. The mutant had mutations in three amino acids: Gly97 to Ala, Thr 98 to Ile, and Pro 102 to Ser. These mutation sites in Escherichia coli have been studied as significant active sites of glyphosate resistance. However, in our research, they were found to jointly contribute to the improvement of glyphosate tolerance. In addition, the level of glyphosate tolerance in transgenic Arabidopsis confirmed the potentiality of the mutant in breeding glyphosateresistant plants.

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“…Glyphosate is a non-selective herbicide that inhibits the EPSPS (5-enolpyruvylshikimate 3-phosphate synthase) gene (Liu and Cao, 2015). It is a low-cost herbicide with a high-control efficacy of several weeds (Preston and Wakelin, 2008).…”

Section: Introductionmentioning

confidence: 99%

EPSPS variability, gene expression, and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis

Galeano¹,

Barroso²,

Vasconcelos³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Weed resistance to herbicides is a natural phenomenon that exerts selection on individuals in a population. In Brazil, glyphosate resistance was recently detected in Digitaria insularis. The objective of this study was to elucidate mechanisms of weed resistance in this plant, including genetic variability, allelism, amino acid substitutions, gene expression, and enzymatic activity levels. Most of these have not previously been studied in this species. D. insularis DNA sequences were used to analyze genetic variability. cDNA from resistant and susceptible plants was used to identify 2 E. Galeano et al. Genetics and Molecular Research 15 (3): gmr.15038730 mutations, alleles, and 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) expression, using real-time quantitative reverse transcriptionpolymerase chain reaction. In addition, EPSPS activity was measured. We found a decrease in genetic variability between populations related to glyphosate application. Substitutions from proline to threonine and tyrosine to cysteine led to a decrease in EPSPS affinity for the glyphosate. In addition, the EPSPS enzymatic activity was slightly higher in resistant plants, whereas EPSPS gene expression was almost identical in both biotypes, suggesting feedback regulation at different levels. To conclude, our results suggest new molecular mechanisms used by D. insularis to increase glyphosate resistance.

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Cloning and characterization of 5-enopyruvylshikimate-3-phosphate synthase from Pantoea sp

Cited by 5 publications

References 23 publications

Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca

Cloning and Characterization of 5-enopy ruvylshikimate-3-phosphate Synthase from Fragaria vesca

Improvement of Glyphosate Resistance through Concurrent Mutations in Three Amino Acids of the Pantoea sp. 5-Enolpyruvylshikimate-3- Phosphate Synthase

EPSPS variability, gene expression, and enzymatic activity in glyphosate-resistant biotypes of Digitaria insularis

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