Cloning and characterization of a novel betaine aldehyde dehydrogenase gene from Suaeda corniculata

Wang, Fw; Ml, Wang; Chen, Guo; Wang, N; Xw, Li; H, Chen; Dong, Y-Y; Xf, Chen; Zm, Wang; Hy, Li

doi:10.4238/gmr.15027848

Cited by 19 publications

(9 citation statements)

References 32 publications

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“…TFs play a vital role in connecting the salt-sensory pathways to the various genes required for plant salt-tolerance (Nishiyama et al 2012). These genes play critical roles in regulating plant stress responses, to various abiotic stress factors including salinity (Joshi et al 2016;Wang et al 2016a). Several studies reported the development of transgenic plants with modified TF expression that improved salt-tolerance (Joshi et al 2016).…”

Section: Engineering Regulatory Elements For Increased Salt Tolerancementioning

confidence: 99%

“…Schumacher et al (1999) demonstrated enhanced the salt tolerance in tobacco. Wang et al (2016a) showed that overexpression of a vacuolar-type HC-ATPase C subunit gene from Iris lactea (IrlVHA-c), enhanced the salt tolerance in tobacco. Liu et al (2018) demonstrated that the gene encoding subunit A of the vacuolar H + -ATPase from cotton (GhVHA-A) plays an important role in conferring tolerance to water deficit.…”

Section: Vacuolar Membrane H + -Atpase (V-atpase)mentioning

confidence: 99%

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Engineering salinity tolerance in plants: progress and prospects

Wani

Kumar

Khare

et al. 2020

Planta

163

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Soil salinity exerts significant constraints on global crop production, posing a serious challenge for plant breeders and biotechnologists. The classical transgenic approach for enhancing salinity tolerance in plants revolves by boosting endogenous defence mechanisms, often via a single gene 2 approach, and usually involves the enhanced synthesis of compatible osmolytes, antioxidants, polyamines, maintenance of hormone homeostasis, modification of transporters and/or regulatory proteins, including transcription factors (TFs) and alternative splicing events. Occasionally, genetic manipulation of regulatory proteins or phytohormone levels confers salinity-tolerance, but all these may cause undesired reduction in plant growth and/or yields. In this review, we present and evaluate novel and cutting-edge approaches for engineering salt tolerance in crop plants. First, we cover recent findings regarding the importance of regulatory proteins and transporters, and how they can be used to enhance salt tolerance in crop plants. We also evaluate the importance of halobiomes as a reservoir of genes that can be used for engineering salt-tolerance in glycophytic crops. Additionally, the role of microRNAs as critical post-transcriptional regulators in plant adaptive responses to salt stress are reviewed and their use for engineering salt-tolerant crop plants is critically assessed. The potentials of alternative splicing mechanisms and targeted gene-editing technologies in understanding plant salt-stress responses and developing salt-tolerant crop plants is also discussed.

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Section: Engineering Regulatory Elements For Increased Salt Tolerancementioning

confidence: 99%

Section: Vacuolar Membrane H + -Atpase (V-atpase)mentioning

confidence: 99%

Engineering salinity tolerance in plants: progress and prospects

Wani

Kumar

Khare

et al. 2020

Planta

163

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“…In higher plants, BADH catalyzes the key step of GB biosynthesis 23 . In Ammopiptanthus nanus , endogenous expression of AnBADH is strongly induced by exposure to high salt, drought, ABA, heat, or cold 24 .…”

Section: Discussionmentioning

confidence: 99%

“…Glycine betaine (GB), a bipolar quaternary ammonium compound that acts as an osmoprotectant, accumulates in many plant species in response to various abiotic stresses 22 . GB protects plants from abiotic stresses by maintaining the osmotic balance under various abiotic stresses and by stabilizing the quaternary structure of complex proteins 23 . We observed increases in GB content in cassava following exposure to drought, osmotic, or salt stress treatments.…”

Section: Introductionmentioning

confidence: 99%

Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response

Ren

et al. 2019

Sci Rep

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Cassava ( Manihot esculenta ) is a major staple food, animal feed and energy crop in the tropics and subtropics. It is one of the most drought-tolerant crops, however, the mechanisms of cassava drought tolerance remain unclear. Abscisic acid (ABA)-responsive element (ABRE)-binding factors (ABFs) are transcription factors that regulate expression of target genes involved in plant tolerance to drought, high salinity, and osmotic stress by binding ABRE cis -elements in the promoter regions of these genes. However, there is little information about ABF genes in cassava. A comprehensive analysis of Manihot esculenta ABFs ( MeABFs ) described the phylogeny, genome location, cis -acting elements, expression profiles, and regulatory relationship between these factors and Manihot esculenta betaine aldehyde dehydrogenase genes ( MeBADHs ). Here we conducted genome-wide searches and subsequent molecular cloning to identify seven MeABFs that are distributed unevenly across six chromosomes in cassava. These MeABFs can be clustered into three groups according to their phylogenetic relationships to their Arabidopsis ( Arabidopsis thaliana ) counterparts. Analysis of the 5′-upstream region of MeABFs revealed putative cis -acting elements related to hormone signaling, stress, light, and circadian clock. MeABF expression profiles displayed clear differences among leaf, stem, root, and tuberous root tissues under non-stress and drought, osmotic, or salt stress conditions. Drought stress in cassava leaves and roots, osmotic stress in tuberous roots, and salt stress in stems induced expression of the highest number of MeABFs showing significantly elevated expression. The glycine betaine (GB) content of cassava leaves also was elevated after drought, osmotic, or salt stress treatments. BADH1 is involved in GB synthesis. We show that MeBADH1 promoter sequences contained ABREs and that MeBADH1 expression correlated with MeABF expression profiles in cassava leaves after the three stress treatments. Taken together, these results suggest that in response to various dehydration stresses, MeABFs in cassava may activate transcriptional expression of MeBADH1 by binding the MeBADH1 promoter that in turn promotes GB biosynthesis and accumulation via an increase in MeBADH1 gene expression levels and MeBADH1 enzymatic activity. These responses protect cells against dehydration stresses by preserving an osmotic balance that enhances cassava tolerance to dehydration stresses.

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“…Accumulation of osmolytes such as glycine betaine (GB), which is synthesized in a pathway in which BADH acts as a critical enzyme, has been recently described in plants growing under abiotic stress, including the water deficit [30], contributing to a higher relative water content, the integrity of cell membranes, stabilization of proteins and detoxification [31]. The relationship of overexpression for BADH biosynthesis with the increase in GB levels and greater tolerance to water deficit has already been observed in plants such as Arabidopsis thaliana [31], Sesuvium portulacastrum [32], and Zea mays [33].…”

Section: Proteins Involved In the Response To Water Deficitmentioning

confidence: 99%

Proteomic Profiling and Rhizosphere-Associated Microbial Communities Reveal Adaptive Mechanisms of Dioclea Apurensis Kunth in Eastern Amazon’s Rehabilitating Minelands

Nascimento¹,

Costa²,

Herrera³

et al. 2022

Preprint

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Dioclea apurensis Kunth is native to ferruginous rocky outcrops (known as canga) in the eastern Amazon. Native cangas are considered hotspots of biological diversity and have one of the largest iron ore deposits in the world. There, D. apurensis can grow in post-mining areas where molecular mechanisms and rhizospheric interactions with soil microorganisms are expected to contribute to their establishment in rehabilitating minelands. In this study, we compare the root proteomic profile and rhizosphere-associated bacterial and fungal communities of D. apurensis growing in canga and a rehabilitating mineland to characterize the main mechanisms that allow the growth and establishment in post-mining areas. The results showed that proteins involved in response to oxidative stress, drought, excess of iron, and phosphorus deficiency were more accumulated in canga and, therefore, helped explain its high establishment rates in rehabilitating minelands. Rhizospheric selectivity of microorganisms was more evident in canga. The microbial community structure was mostly different between the two habitats, denoting that despite having its preferences, D. apurensis can associate with beneficial soil microorganisms without specificity. Therefore, its good performance in rehabilitating minelands can also be improved or attributed to its ability to cope with beneficial soil-borne microorganisms. Native plants with such adaptations must be used to enhance the rehabilitation process.

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Cloning and characterization of a novel betaine aldehyde dehydrogenase gene from Suaeda corniculata

Cited by 19 publications

References 32 publications

Engineering salinity tolerance in plants: progress and prospects

Engineering salinity tolerance in plants: progress and prospects

Involvement of abscisic acid-responsive element-binding factors in cassava (Manihot esculenta) dehydration stress response

Proteomic Profiling and Rhizosphere-Associated Microbial Communities Reveal Adaptive Mechanisms of Dioclea Apurensis Kunth in Eastern Amazon’s Rehabilitating Minelands

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