Cloning and characterization of a gene encoding a new thermostable hemolysin from<i>Vibrio parahaemolyticus</i>

Taniguchi, Hatsumi; Kubomura, Shigeo; Hirano, Hideaki; Mizue, Kumiko; Ogawa, Midori; Mizuguchi, Yasuo

doi:10.1111/j.1574-6968.1990.tb04044.x

Cited by 18 publications

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“…Vibrio parahaemolyticus is a marine bacterium that can cause gastroenteritis, the major clinical symptoms being diarrhea, abdominal cramps, nausea, and vomiting (1, 17,19). Although the pathogenic mechanisms of this organism are not well understood, proposed virulence factors or properties include thermostable direct hemolysin (TDH), other extracellular or cell-associated toxins or enzymes (3,14,16,17,19,21,34,36,38,39), adherence (13,17,19,20), and invasiveness (4,19). Of these potential virulence factors, TDH has been considered very important because of a striking epidemiological correlation with the Kanagawa phenomenon (KP), which is beta-hemolysis induced by TDH in a special blood agar medium (Wagatsuma agar).…”

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Enterotoxigenicity of Vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin

et al. 1992

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Vibrio parmhaemolyticus produces a thermostable direct hemolysin (TDH) that has been implicated in the pathogenesis of diarrheal disease caused by this organism. However, previous studies attempting to demonstrate the contribution of the hemolysin to virulence have been inconclusive. We investigated this putative virulence factor by using an isogenic TDH-negative (DH-) strain constructed by specifically inactivating the two copies of the tdh gene encoding TDH. The enterotoxigenicities of the parent strain (AQ3815) and the mutant strain were tested by adding sterile culture supernatants to rabbit ileal tissue mounted in Ussing chambers. The culture filtrate of the parent strain produced a significant increase in short-circuit current (Isc), compared with the change induced by the TDH-mutant. The capacity of the culture filtrate of AQ3815 to increase the Isc was reduced by neutralization with anti-TDH serum, and the return of the cloned tdh gene to the TDH-mutant restored the ability to increase the Isc. These results were corroborated by rabbit ileal loop assays in which AQ3815 caused fluid accumulation but the TDHmutant did not. No microscopic damage was seen in mucosal tissues exposed to the culture filtrate of either strain. These results indicate that TDH has an enterotoxigenic effect on rabbit small intestine and could be responsible for the watery diarrhea seen with V. parahaemolyticus.

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Enterotoxigenicity of Vibrio parahaemolyticus with and without genes encoding thermostable direct hemolysin

et al. 1992

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“…The marine strain was isolated from the edible blue mussel, Mytilus edulis , collected outside Sven Lovéns Center for Marine Infrastructure on the Swedish west coast. This marine strain lacks the tdh and trh genes, but it does harbor the σ-VPH hemolysin gene (3). According to an established 7-loci multilocus sequence typing (MLST) scheme (4), this strain has a unique recA allele (no.…”

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confidence: 99%