Cloning and characterization of a cell cycle-regulated gene encoding Topoisomerase I from Nicotiana tabacum that is inducible by light, low temperature and abscisic acid

Mudgil, Yashwanti; Singh, Bharat; Upadhyaya, Kailash C.; Sopory, Sudhir K.; Reddy, M. K.

doi:10.1007/s00438-002-0669-2

Cited by 20 publications

(7 citation statements)

References 57 publications

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“…The percentage of cellular mortality and expression of TOP1 isoforms were assessed to gain more insight into cell behaviour during treatments. As DNA topoisomerase I is involved in solving the conformational changes in DNA topology, it plays essential roles in several cellular processes (e.g., replication, transcription, recombination) [ 34 ] as well as in the response to stress agents [ 35 , 36 , 37 ]. It can also act as a damage sensor and cofactor in DNA repair pathways [ 38 , 39 ].…”

Section: Discussionmentioning

confidence: 99%

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

Sabatini¹,

Pagano²,

Araújo

et al. 2017

Genes

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Tyrosyl-DNA phosphodiesterase 1 (Tdp1) is involved in DNA repair pathways as it mends the topoisomerase I—DNA covalent complexes. In plants, a small Tdp1 gene family, composed by Tdp1α and Tdp1β genes, was identified, but the roles of these genes in abiotic stress responses are not fully understood. To investigate their specific stress response patterns, the present study made use of bioinformatic and molecular tools to look into the Tdp1β gene function, so far described only in the plant kingdom, and compare it with Tdp1α gene coding for the canonical, highly conserved α isoform. The expression profiles of Tdp1α and Tdp1β genes were examined under abiotic stress conditions (cold, heat, high osmolarity, salt, and UV-B) in two model species, Arabidopsis thaliana and Medicago truncatula. The two isoforms of topoisomerase I (TOP1α and TOP1β) were also taken into consideration in view of their known roles in DNA metabolism and cell proliferation. Data relative to gene expression in Arabidopsis were retrieved from the AtGenExpress microarray dataset, while quantitative Real-Time PCR was carried out to evaluate the stress response in M. truncatula cell cultures. These analyses revealed that Tdp1β gene expression was enhanced during the first hour of treatment, whereas Tdp1α enhanced expression succeeded at subsequent timepoints. In agreement with the gene-specific responses to abiotic stress conditions, the promoter regions of Tdp1α and Tdp1β genes are well equipped with stress-related cis-elements. An in-depth bioinformatic characterization of the HIRAN motif, a distinctive feature of the Tdp1β protein, showed its wide distribution in chromatin remodeling and DNA repair proteins. The reported data suggests that Tdp1β functions in the early response to abiotic stresses.

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Section: Discussionmentioning

confidence: 99%

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

Sabatini¹,

Pagano²,

Araújo

et al. 2017

Genes

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“…DSBs can be generated in the course of transposition and/or by specific enzymes such as topoisomerases (reviewed in Wang, 2002) and single-stranded DNA endonucleases that target weakly hydrogen bonded regions (e.g., AT-rich regions) within double strand DNA (Grafi and Larkins, 1995). Interestingly, topoisomerases are upregulated in plants following exposure to various stress conditions (Mudgil et al, 2002; Hettiarachchi et al, 2005) and their activities might be necessary for proper function of the meristem (Graf et al, 2010). Apparently, these HR events are stress-induced (Table 1) and could irreversibly modify the genome often leading to somaclonal variation in plants and to diseases in humans (Kaeppler et al, 2000; Griffin and Thacker, 2004; Hurles, 2005).…”

Section: Stress-induced Dedifferentiation and Genetic Variationmentioning

confidence: 99%

The Stem Cell State in Plant Development and in Response to Stress

Grafi¹,

Florentin²,

Ransbotyn³

et al. 2011

Front. Plant Sci.

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Stem cells are commonly defined by their developmental capabilities, namely, self-renewal and multitype differentiation, yet the biology of stem cells and their inherent features both in plants and animals are only beginning to be elucidated. In this review article we highlight the stem cell state in plants with reference to animals and the plastic nature of plant somatic cells often referred to as totipotency as well as the essence of cellular dedifferentiation. Based on recent published data, we illustrate the picture of stem cells with emphasis on their open chromatin conformation. We discuss the process of dedifferentiation and highlight its transient nature, its distinction from re-entry into the cell cycle and its activation following exposure to stress. We also discuss the potential hazard that can be brought about by stress-induced dedifferentiation and its major impact on the genome, which can undergo stochastic, abnormal reorganization leading to genetic variation by means of DNA transposition and/or DNA recombination.

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“…Early observations of ABA on Arabidopsis cell division come from BrdU incorporation analysis (Robertson et al, 1990;Leung et al, 1994). ABA appears to have a direct role in repressing the expression of some cell cycle genes, such as CDKA (formerly cdc2a; Hemerly et al, 1993) or DNA replication genes, such as the DNA replication initiation CDT1 (Castellano et al, 2004), topoisomerase I (Mudgil et al, 2002) or telomerase (Yang et al, 2002). In addition, ABA activates the expression of other genes, which are repressors of cell division, such as the CDK inhibitor KRP1/ICK1 (Wang et al, 1998).…”

Section: Abscisic Acid (Aba)mentioning

confidence: 99%

The Arabidopsis Cell Division Cycle

Gutiérrez

2009

The Arabidopsis Book

165

134

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Plant cells have evolved a complex circuitry to regulate cell division. In many aspects, the plant cell cycle follows a basic strategy similar to other eukaryotes. However, several key issues are unique to plant cells. In this chapter, both the conserved and unique cellular and molecular properties of the plant cell cycle are reviewed. In addition to division of individual cells, the specific characteristic of plant organogenesis and development make that cell proliferation control is of primary importance during development. Therefore, special attention should be given to consider plant cell division control in a developmental context. Proper organogenesis depends on the formation of different cell types. In plants, many of the processes leading to cell differentiation rely on the occurrence of a different cycle, termed the endoreplication cycle, whereby cells undergo repeated full genome duplication events in the absence of mitosis and increase their ploidy. Recent findings are focusing on the relevance of changes in chromatin organization for a correct cell cycle progression and, conversely, in the relevance of a correct functioning of chromatin remodelling complexes to prevent alterations in both the cell cycle and the endocycle.

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Cloning and characterization of a cell cycle-regulated gene encoding Topoisomerase I from Nicotiana tabacum that is inducible by light, low temperature and abscisic acid

Cited by 20 publications

References 57 publications

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

The Tyrosyl-DNA Phosphodiesterase 1β (Tdp1β) Gene Discloses an Early Response to Abiotic Stresses

The Stem Cell State in Plant Development and in Response to Stress

The Arabidopsis Cell Division Cycle

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