Cloning and characterization of a benzoic acid/salicylic acid carboxyl methyltransferase gene involved in floral scent production from lily (Lilium ‘Yelloween’)

Wang, H; Sun, Min; Ll, Li; Xh, Xie; Qx, Zhang

doi:10.4238/2015.november.18.14

Cited by 14 publications

(9 citation statements)

References 27 publications

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“…Activity with SA and BA has likely been maintained since relatively early in the history of the monocot lineage because the BSMT orthologs we characterized from Musa and Phalaenopsis (Zingiberales and Asparagales, respectively) show activity with both substrates ( fig. 4 ) as does a previously reported one from Lilium (Liliales) ( Wang et al 2015 ). Second, the phylogenetic analysis and characterization of a Carica SAMT sequence ( fig.…”

Section: Resultssupporting

confidence: 82%

A Collaborative Classroom Investigation of the Evolution of SABATH Methyltransferase Substrate Preference Shifts over 120 My of Flowering Plant History

Dubs

Davis

Brito

et al. 2022

Molecular Biology and Evolution

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Next-generation sequencing has resulted in an explosion of available data, much of which remains unstudied in terms of biochemical function; yet, experimental characterization of these sequences has the potential to provide unprecedented insight into the evolution of enzyme activity. One way to make inroads into the experimental study of the voluminous data available is to engage students by integrating teaching and research in a college classroom such that eventually hundreds or thousands of enzymes may be characterized. In this study, we capitalize on this potential to focus on SABATH methyltransferase enzymes that have been shown to methylate the important plant hormone, salicylic acid (SA), to form methyl salicylate. We analyze data from 76 enzymes of flowering plant species in 23 orders and 41 families to investigate how widely conserved substrate preference is for salicylic acid methyltransferase (SAMT) orthologs. We find a high degree of conservation of substrate preference for SA over the structurally similar metabolite, benzoic acid, with recent switches that appear to be associated with gene duplication and at least three cases of functional compensation by paralogous enzymes. The presence of Met in active site position 150 is a useful predictor of SA methylation preference in SABATH methyltransferases but enzymes with other residues in the homologous position show the same substrate preference. Although our dense and systematic sampling of SABATH enzymes across angiosperms has revealed novel insights, this is merely the “tip of the iceberg” since thousands of sequences remain uncharacterized in this enzyme family alone.

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Section: Resultssupporting

confidence: 82%

A Collaborative Classroom Investigation of the Evolution of SABATH Methyltransferase Substrate Preference Shifts over 120 My of Flowering Plant History

Dubs

Davis

Brito

et al. 2022

Molecular Biology and Evolution

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“…S22a-27a). It has been confirmed that SAMT is a key gene regulating the synthesis of floral aromatic VOCs in various plant species, such as Clarkia amoena, Lilium, Jasminum sambac, and Eriobotrya japonica [36][37][38][39][40][41]. The release of methyl salicylate peaked at the flower-bud stage and remained stable throughout the floral growth and developmental stages, and SAMT expression was downregulated after the petals opened.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic and metabolomic data reveal key genes that are involved in the phenylpropanoid pathway and regulate the floral fragrance of Rhododendron fortunei

et al. 2023

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Background To reveal the key genes involved in the phenylpropanoid pathway, which ultimately governs the fragrance of Rhododendron fortunei, we performed a comprehensive transcriptome and metabolomic analysis of the petals of two different varieties of two alpine rhododendrons: the scented R. fortunei and the unscented Rhododendron ‘Nova Zembla’. Results Our transcriptomic and qRT–PCR data showed that nine candidate genes were highly expressed in R. fortunei but were downregulated in Rhododendron ‘Nova Zembla’. Among these genes, EGS expression was significantly positively correlated with various volatile benzene/phenylpropanoid compounds and significantly negatively correlated with the contents of various nonvolatile compounds, whereas CCoAOMT, PAL, C4H, and BALDH expression was significantly negatively correlated with the contents of various volatile benzene/phenylpropanoid compounds and significantly positively correlated with the contents of various nonvolatile compounds. CCR, CAD, 4CL, and SAMT expression was significantly negatively correlated with the contents of various benzene/phenylpropanoid compounds. The validation of RfSAMT showed that the RfSAMT gene regulates the synthesis of aromatic metabolites in R. fortunei. Conclusion The findings of this study indicated that key candidate genes and metabolites involved in the phenylpropanoid biosynthesis pathway may govern the fragrance of R. fortunei. This lays a foundation for further research on the molecular mechanism underlying fragrance in the genus Rhododendron.

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“…The expression of PAL was down-regulated in YJB and YJC, it was signi cantly negatively correlated with various benzene-like VOC, and signi cantly positively correlated with non-volatile benzene-like substances. Verdonk et al [45] used DNA microarray analysis and found that the key enzyme PAL for benzene synthesis was found to be transcriptionally induced before the onset of VOC release, and then combining HS-SPME-GC-MS technology and targeted metabolomics method to monitor VOC release in petunia hybrid W115. Finally, Verdonk et al found that PAL regulates the production of petunia aroma VOC.…”

Section: Discussionmentioning

confidence: 99%

“…The expression of SAMT was up-regulated in YJD and was signi cantly negatively correlated with benzene VOC. It has been con rmed that SAMT is a key gene regulating the synthesis of oral aroma VOC in various plants such as Clarkia amoena, Lilium, Jasminum sambac, and Eriobotrya japonica [43][44][45][46][47][48]. Dudareva et al [43] found that petals are the main tissue site for the release of methyl salicylate, and the activity of salicylate carboxymethyl transferase was the highest in petal tissues, and peaked in petals during the blooming period a few days after owering decreased, which was strongly positively correlated with the release of methyl salicylate.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome and metabolome reveal key genes in the phenylpropane pathway to regulate the floral fragrance of Rhododendron fortunei

Yang

Qin

Jia

et al. 2022

Preprint

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Background: Floral aroma is an important plant trait and it is one of the main traits of Rhododendron varieties which affect their ornamental value. The volatile compounds of floral aroma are secondary metabolites. In order to reveal the key genes of the phenylpropane pathway, which regulates the fragrance of Rhododendron fortunei, we performed a comprehensive transcriptome and metabolome analysis of the petals of the petals of two alpine rhododendrons from two different varieties: the scented Rhododendron fortunei and the non-scented Rhododendron ‘Nova Zembla’. Results: Transcriptomic and qRT-PCR results showed that nine candidate genes were highly expressed in R. fortunei, but down-regulated in R. ‘Nova Zembla’. The correlation analysis of candidate genes and metabolites revealed nine key genes involved in the regulation of floral aroma in the phenylpropane pathway. Among them, EGS was significantly positively correlated with various volatile benzene/phenylpropane compounds, and significantly negatively correlated with various non-volatile compounds; CCoAOMT, PAL, C4H,and BALDH were significantly negatively correlated with various volatile benzene/phenylpropane compounds, and significantly positively correlated with various non-volatile compounds. CCR, CAD, 4CL, and EGSwere significantly negatively correlated with various benzene/phenylpropane compounds. The validation of RfSAMT proved that RfSAMT gene regulated the synthesis of aromatic substances in R. fortunei. Conclusion: The findings of this study indicated that key candidate genes and metabolites involved in the biosynthetic pathway of phenylpropane may regulate the fragrance of R. fortunei. This lays a foundation for further research on the molecular mechanism of fragrance synthesis in the genus Rhododendron.

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Cloning and characterization of a benzoic acid/salicylic acid carboxyl methyltransferase gene involved in floral scent production from lily (Lilium ‘Yelloween’)

Cited by 14 publications

References 27 publications

A Collaborative Classroom Investigation of the Evolution of SABATH Methyltransferase Substrate Preference Shifts over 120 My of Flowering Plant History

A Collaborative Classroom Investigation of the Evolution of SABATH Methyltransferase Substrate Preference Shifts over 120 My of Flowering Plant History

Transcriptomic and metabolomic data reveal key genes that are involved in the phenylpropanoid pathway and regulate the floral fragrance of Rhododendron fortunei

Transcriptome and metabolome reveal key genes in the phenylpropane pathway to regulate the floral fragrance of Rhododendron fortunei

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