Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici

Liu, Bo; Xue, Xiao‐Feng; Cui, Suping; Zhang, Xiaoyu; Han, Qingmei; Zhu, Lin; Liang, Xiaofei; Wang, Xiaojie; Huang, Lili; Chen, Xianming; Kang, Zhensheng

doi:10.1007/s11033-009-9823-9

Cited by 76 publications

(67 citation statements)

References 19 publications

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“…One full length cDNA of β-1, 3-glucosidase was cloned from a cotton cultivar "Hai7124" (Gao et al, 2007). The coding region of β-1,3-glucosidase was cloned from wheat induced by stripe rust (Liu et al, 2007). Lipid transfer protein plays a role not only in lipid transfer and keratogenesis but also in the plant defense response (Gomes et al, 2003;Maldonado et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Expression of special genes inhibited by powdery mildew (Blumeria graminis f. sp. tritici) in wheat germplasm N9436

et al. 2010

Front. Agric. China

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Powdery mildew, caused by Blumeria graminis f. sp. tritici, is one of the most important fungal diseases in common wheat (Triticum aestivum L.) worldwide. Wheat germplasm N9436 is resistant to powdery mildew. In the present study, a backward subtracted cDNA library was constructed with cDNA from N9436 leaves inoculated by Blumeria graminis as the driver and cDNA from uninoculated N9436 leaves as the tester. A total of 120 positive clones were randomly chosen from the SSHcDNA library and were amplified with sp6 and t7 primers to examine the insert size. After screening the repeated and redundant sequences, 59 expressed sequence tags (EST) were acquired. Nucleic acid and protein homology search were performed using the basic local alignment search tool (BLAST) program with the default settings at NCBI website (http://www.ncbi.nlm.nih.gov). BlastX results in nr-protein database revealed that 23 ESTs were highly homologous with known proteins involved in primary metabolism, energy metabolism, transport, signal transduction, and disease resistance and defenses. BlastNr results showed that 47 and 10 ESTs had high identities with known Unigene and function-unknown ESTs, respectively, and two ESTs matched none in the nrdatabase. Twenty-one ESTs were both in the nucleic acid and protein databases, including seven ESTs associated with powdery mildew resistance. Among them, one was responsible for signal transduction and six for systemic acquired resistance (SAR) system.

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Section: Discussionmentioning

confidence: 99%

Expression of special genes inhibited by powdery mildew (Blumeria graminis f. sp. tritici) in wheat germplasm N9436

et al. 2010

Front. Agric. China

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“…It can be hypothesized that a major role for GIP is to suppress the release of glucan elicitors during P. cinnamomi infection, thereby reducing the effectiveness of the plant host's surveillance system [19,21].…”

Section: Amplification and Characterization Of The Gip Gene Of P Cinmentioning

confidence: 99%

Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

Martins

Belo

et al. 2014

Mol Biol Rep

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Oomycetes from the genus Phytophthora are fungus-like plant pathogens that are devastating for agriculture and natural ecosystems. They are able to secrete a glucanase inhibitor protein (GIP) that inhibits the activity of endoglucanases (EGases) involved in defense responses against infection. One of the most widely distributed and aggressive Phytophthora species, with more than 1,000 host plants is P. cinnamomi. In this work we report the sequencing and characterization of a class of GIPs secreted by Phytophthora cinnamomi. The gip gene from P. cinnamomi has a 937 bp ORF encoding a putative peptide of 312 deduced amino acids. The expression of this gene was studied during growth in different carbon sources (glucose, cellulose and sawdust), by RT-qPCR and its level of expression was evaluated at five time points. The highest expression of gip gene occurred in sawdust at 8 h of induction. In vivo infection of C. sativa revealed an increase in gip expression from 12 to 24 h. At 36 h its expression decreased suggesting that a compensatory mechanism must occur in plant.

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“…Thaumatin like protein [33] and β-1,3-glucanase [34] were upregulated in both compatible and incompatible wheat-Pst interactions, during which they may participate in the basal defense of wheat against Pst. The higher level of transcription and greater abundance of protein labeling in the incompatible wheat-Pst interaction than in the compatible interaction further suggested their positive role in wheat resistance to Pst [33,34] . Combined with the induced expression in response to phytohormones (SA, jasmonic acid and abscisic acid), it is reasonable to suggest that these two PR proteins are involved in both SAR and induced systemic resistance.…”

Section: Pathogenesis-related Proteinsmentioning

confidence: 99%

New insights in the battle between wheat and Puccinia striiformis

Tang¹,

Wang²,

Cheng³

et al. 2015

Front. Agr. Sci. Eng.

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Wheat stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) poses a great threat to wheat production worldwide. The rapid change in virulence of Pst leads to a loss of resistance in currently resistant wheat cultivars, which results in frequent disease epidemics. Therefore, a major focus is currently placed on investigating the molecular mechanisms underlying this rapid variation of pathogenicity and coevolving wheat resistance. Limited by the lack of a system for stable transformation of Pst and the difficulties in wheat transformation, it is not easy to generate deeper insights into the wheat-Pst interaction using established genetic methods. Nevertheless, considerable effort has been made to unravel the wheat-Pst interaction and significant progress is being made. Histology and cytology have revealed basic details of infection strategies and defense responses during wheat-Pst interactions, identified cellular components involved in wheat-Pst interactions, and have helped to elucidate their role in the infection process or in plant defense responses. Transcriptome and genome sequencing has revealed the molecular features and dynamics of the wheat-Pst pathosystem. Extensive molecular analyses have led to the identification of major components in the wheat resistance response and in Pst virulence. Studies of wheat-Pst interactions have now entered a new phase in which cellular and molecular approaches are being used. This review focuses on the cellular biology of wheat-Pst interactions and integrates the emerging data from molecular analyses with the histocytological observations.

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Cloning and characterization of a wheat β-1,3-glucanase gene induced by the stripe rust pathogen Puccinia striiformis f. sp. tritici

Cited by 76 publications

References 19 publications

Expression of special genes inhibited by powdery mildew (Blumeria graminis f. sp. tritici) in wheat germplasm N9436

Expression of special genes inhibited by powdery mildew (Blumeria graminis f. sp. tritici) in wheat germplasm N9436

Cloning, characterization and in vitro and in planta expression of a glucanase inhibitor protein (GIP) of Phytophthora cinnamomi

New insights in the battle between wheat and Puccinia striiformis

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