Cloning and Characterization of a β-Glucosidase from Marine Microbial Metagenome with Excellent Glucose Tolerance

Fang, Zemin; Fang, Wei; Liu, Juanjuan; Hong, Yuzhi; Peng, Hui; Zhang, Xuecheng; Sun, Baolin; Xiao, Yazhong

doi:10.4014/jmb.1003.03011

Cited by 121 publications

(72 citation statements)

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“…Unbgl1A shows a high tolerance to high concentration of NaCl. It was previously reported that 500 mM NaCl could decrease 14% activity of the b-glucosidase BglA, which was obtained from marine microbial metagenome [30]. In contrast, in the present study, the activity of Unbgl1A was not affected by as much as 600 mM NaCl.…”

Section: Transglucosylationcontrasting

confidence: 54%

“…Till today, only a handful of microorganisms have been found to embody b-glucosidases showing high glucose tolerance with K i values of more than 500 mM [27][28][29][30][31]. For example, b-glucosidases from marine microbial metagenome displayed high inhibition constant for glucose with K i of 1000 mM [30], and only at glucose concentrations .300 mM, the b-glucosidase activity from Streptomyces sp. QM-B814 was slightly decreased [31].…”

Section: Transglucosylationmentioning

confidence: 99%

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Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Lü¹,

Du²,

Wei³

et al. 2013

ABBS

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A b-glucosidase gene unbgl1A was isolated by the functionbased screening of a metagenomic library and the enzyme protein was expressed in Escherichia coli, purified, and biochemically characterized. The enzyme Unbgl1A had a K m value of 2.09 + + + + + 0.31 mM, and a V max value of 183.90 + + + + + 9.61 mmol min 21 mg 21 under the optimal reaction conditions, which were pH 6.0 at 508 8 8 8 8C. Unbgl1A can be activated by a variety of monosaccharides, disaccharides, and NaCl, and exhibits a high level of stability at high concentration of NaCl. Two prominent features for this enzyme are: (i) high glucose tolerance. It can be tolerant to glucose as high as 2000 mM, with K i 5 1500 mM; (ii) high NaCl tolerance. Its activity is not affected by 600 mM NaCl. The enzyme showed transglucosylation activities resulting in the formation of cellotriose from cellobiose. These properties of Unbgl1A should have important practical implication in its potential applications for better industrial production of glucose or bioethanol started from lignocellulosic biomass.

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Section: Transglucosylationcontrasting

confidence: 54%

Section: Transglucosylationmentioning

confidence: 99%

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Lü¹,

Du²,

Wei³

et al. 2013

ABBS

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“…For examples, β-glucosidase from Microbispora bispora was activated in the presence of 300 mM glucose [135] and that reported in T. thermosaccharolyticum DSM 571 and T. aotearoense was activated by glucose concentration < 200 and 250 mM, respectively [85,136]. Similarly, recombinant GH 1 β-glucosidase from Exiguobacterium antarcticum B7 [84], Fervidobacterium islandicum [137], Thermotoga thermarum DSM 5069T [138] and Anoxybacillus DT3-1 [83] was tolerant to high glucose concentration e.g., Ki of 0.54-15 M. On the other hand, glucose tolerant β-glucosidase with Ki of 1.0-4.8 M has been identified from different environments using metagenomic approaches [45,53,106,111,139,140].…”

Section: Glucose Sensitivity and Tolerancementioning

confidence: 99%

“…For examples, Kwon et al used esculin to visualize β-glucosidase activity on Native-PAGE. The gel was removed and soaked in solution containing 0.1% esculin and 0.03% ferric ammonium citrate until a dark band appeared on the gel indicating the presence of β-glucosidase activity [139,166,167]. P-PNG has also been utilized to detect β-glucosidase activity on PAGE.…”

Section: Zymographymentioning

confidence: 99%

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Ahmed¹,

Aslam²,

Ashraf³

et al. 2017

JAEM

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Cellulose is the most abundant biomaterial in the biosphere and the major component of plant biomass.Cellulase is an enzymatic system required for conversion of renewable cellulose biomass into free sugar for subsequent use in different applications. Cellulase system mainly consists of three individual enzymes namely: endoglucanase, exoglucanase and β-glucosidases. β-Glucosidases are ubiquitous enzymes found in all living organisms with great biological significance. β-Glucosidases have also tremendous biotechnological applications such as biofuel production, beverage industry, food industry, cassava detoxification and oligosaccharides synthesis. Microbial β-glucosidases are preferred for industrial uses because of robust activity and novel properties exhibited by them. This review aims at describing the various biochemical methods used for screening and evaluating β-glucosidases activity from microbial sources. Subsequently, it generally highlights techniques used for purification of β-glucosidases. It then elaborates various biochemical and molecular properties of this valuable enzyme such as pH and temperature optima, glucose tolerance, substrate specificity, molecular weight, and multiplicity. Furthermore, it describes molecular cloning and expression of bacterial, fungal and metagenomic β-glucosidases. Finally, it highlights the potential biotechnological applications of β-glucosidases.

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“…r glucosidase = −0.43, r phenol oxidase = −0.43 with all p ≤ 0.04; r xylosidase = −0.13, p = 0.39), suggesting that salinity itself may have adverse effects on enzyme activity. Work with purified enzymes has shown that elevated salinity can reduce both phenol oxidase (Das et al, 2001) and glucosidase activity (Fang et al, 2010). There is no evidence in the literature that cellobiosidase activity responds to ionic strength (BRENDA, 2013).…”

Section: Extracellular Enzyme Activitymentioning

confidence: 99%

Saltwater intrusion into tidal freshwater marshes alters the biogeochemical processing of organic carbon

2013

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Abstract. Environmental perturbations in wetlands affect the integrated plant-microbial-soil system, causing biogeochemical responses that can manifest at local to global scales. The objective of this study was to determine how saltwater intrusion affects carbon mineralization and greenhouse gas production in coastal wetlands. Working with tidal freshwater marsh soils that had experienced ∼ 3.5 yr of in situ saltwater additions, we quantified changes in soil properties, measured extracellular enzyme activity associated with organic matter breakdown, and determined potential rates of anaerobic carbon dioxide (CO 2 ) and methane (CH 4 ) production. Soils from the field plots treated with brackish water had lower carbon content and higher C : N ratios than soils from freshwater plots, indicating that saltwater intrusion reduced carbon availability and increased organic matter recalcitrance. This was reflected in reduced activities of enzymes associated with the hydrolysis of cellulose and the oxidation of lignin, leading to reduced rates of soil CO 2 and CH 4 production. The effects of long-term saltwater additions contrasted with the effects of short-term exposure to brackish water during three-day laboratory incubations, which increased rates of CO 2 production but lowered rates of CH 4 production. Collectively, our data suggest that the long-term effect of saltwater intrusion on soil CO 2 production is indirect, mediated through the effects of elevated salinity on the quantity and quality of autochthonous organic matter inputs to the soil. In contrast, salinity, organic matter content, and enzyme activities directly influence CH 4 production. Our analyses demonstrate that saltwater intrusion into tidal freshwater marshes affects the entire process of carbon mineralization, from the availability of organic carbon through its terminal metabolism to CO 2 and/or CH 4 , and illustrate that long-term shifts in biogeochemical functioning are not necessarily consistent with short-term disturbance-type responses.

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Cloning and Characterization of a β-Glucosidase from Marine Microbial Metagenome with Excellent Glucose Tolerance

Cited by 121 publications

References 0 publications

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Saltwater intrusion into tidal freshwater marshes alters the biogeochemical processing of organic carbon

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Cloning and Characterization of a β-Glucosidase from Marine Microbial Metagenome with Excellent Glucose Tolerance

Cited by 121 publications

References 0 publications

Expression and characterization of a novel highly glucose-tolerant &beta;-glucosidase from a soil metagenome

Expression and characterization of a novel highly glucose-tolerant &beta;-glucosidase from a soil metagenome

Microbial β-Glucosidases: Screening, Characterization, Cloning and Applications

Saltwater intrusion into tidal freshwater marshes alters the biogeochemical processing of organic carbon

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Product

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Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome

Expression and characterization of a novel highly glucose-tolerant β-glucosidase from a soil metagenome