Cloning and characterization of a carbohydrate metabolism-associated gene IbSnRK1 from sweetpotato

A sucrose non‐fermenting‐1‐related protein kinase‐1 gene, IbSnRK1, improves starch content, composition, granule size, degree of crystallinity and gelatinization in transgenic sweet potato

“…SbSnRK1α from the wild potato species Solanum berthaultii had significant effects on StvacINV1‐associated sucrose degradation in transgenic potato (Lin et al ., ). The expression levels of SuSy and AGPase were increased and the activities of both enzymes were also enhanced in the IbSnRK1 ‐overexpressing tobacco plants (Jiang et al ., ). Overexpression of StSnRK1 from potato up‐regulated SuSy , AGPase and SS III and enhanced the activities of the corresponding enzymes in transgenic tobacco (Wang et al ., ).…”

“…In our previous study, one IbSnRK1 gene was isolated from sweet potato cv. Lushu 3 (Jiang et al ., ), and its overexpression increased the nitrate N content in roots and soluble protein and starch contents in leaves of transgenic sweet potato (Ren et al ., ). In this study, we found that its overexpression not only increased starch content, but also decreased proportion of amylose, enlarged granule size and improved degree of crystallinity and gelatinization in the storage roots of transgenic sweet potato.…”

“…Sweet potato, Ipomoea batatas (L.) Lam., is an important starch crop. Its potential as food and carbohydrate source has been widely recognized (Jarret et al, 1992;Jiang et al, 2013). Improving starch content and quality of this crop remains an urgent demand, especially in the field of biotechnology.…”

Constitutive Expression of <i>VvAATP</i> Increases Starch Content in Transgenic <i>Arabidopsis</i>

“…These results suggested that constitutive expression of VvAATP enhanced the starch biosynthesis due to the increased ATP supply into amyloplasts, which further increased the production of precursors (ADPG and G-1-P) and the expression of starch biosynthesis related genes ( Figure 6). The higher level of starch content is related to the increased expression of starch biosynthesis genes [5,[31][32][33][34]. Starch synthase can be grouped into five types, granule-bound starch synthase (GBSS) and four types of soluble starch synthases (SSS): SSS I, SSS II, SSS III and SSS IV [5,6,35].…”

“…Starch synthase can be grouped into five types, granule-bound starch synthase (GBSS) and four types of soluble starch synthases (SSS): SSS I, SSS II, SSS III and SSS IV [5,6,35]. A large body of evidence has illustrated that up-regulation of these genes can increase starch accumulation in plants [31,[35][36][37][38]. In this study, systematic up-regulation of these genes (AtGBSS I, AtGBSS II, AtSSS I, AtSSS II, AtSSS III, AtSSS IV, AtSBE I and AtSBE II) involved in starch biosynthesis pathway was observed in transgenic plants (Figure 4).…”