Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum

Zhao, Jianmin; Qiu, Lihua; Ning, Xuanxuan; Chen, Aiqin; Wu, Huifeng; Li, Chenghua

doi:10.1016/j.cbpb.2010.02.001

Cited by 49 publications

(22 citation statements)

References 39 publications

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“…In the present study, total haemocyte was used as our target tissue to examine the expression profiles of VpAIF-1 after bacteria challenge. During the early phase of Vibrio infection, VpAIF-1 transcript was decreased significantly in circulating haemocytes was probably be explained that there are massive granular haemocyte infiltrated at the site of infection and the neutralized localized infection resulted in lowering the number of circulating immune cells temporarily [28]. The similar phenomenon in AIF-1 expression had been described in rat [10] and red seabream [11].…”

Section: Discussionsupporting

confidence: 57%

Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum

Zhang

Zhao

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionsupporting

confidence: 57%

Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum

Zhang

Zhao

et al. 2011

Fish & Shellfish Immunology

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“…defensin and lysozyme are antibacterial components that have been characterized in marine mollusks [40,41]. In this work, the significant up-regulation of mRNA expression levels of Cu/Zn-SOD, HSP90, defensin and lysozyme indicated the oxidative and immune stresses induced by V. harveyi in both digestive gland and gill samples from V. harveyi-challenged mussels.…”

Section: Stress-responsive Gene Expressions In Digestive Gland and Gimentioning

confidence: 75%

Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge

Liu

Zhao

et al. 2014

Fish & Shellfish Immunology

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a b s t r a c tMussel Mytilus galloprovincialis is a marine aquaculture shellfish distributing widely along the coast in north China. In this work, we studied the differential metabolic responses induced by Vibrio harveyi in digestive gland and gill tissues from M. galloprovincialis using NMR-based metabolomics. The differential metabolic responses in the two tissue types were detected, except the similarly altered taurine and betaine. These metabolic responses suggested that V. harveyi mainly induced osmotic disruption and reduced energy demand via the metabolic pathways of glucose synthesis and ATP/AMP conversion in mussel digestive gland. In mussel gill tissues, V. harveyi basically caused osmotic stress and possible reduced energy demand as shown by the elevated phosphocholine that is involved in one of the metabolic pathways of ATP synthesis from ADP and phosphocholine. The altered mRNA expression levels of related genes (superoxide dismutase with copper and zinc, heat shock protein 90, defensin and lysozyme) suggested that V. harveyi induced clear oxidative and immune stresses in both digestive gland and gill tissues. However, the mRNA expression levels of both lysozyme and defensin in digestive gland were more significantly up-regulated than those in gill from V. harveyi-challenged mussel M. galloprovincialis, meaning that the immune organ, digestive gland, was more sensitive than gill. Overall, our results indicated that V. harveyi could induce tissue-specific metabolic responses in mussel M. galloprovincialis.

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“…Therefore, it is necessary to carry out biomonitoring for heavy metal pollution in pollution on the basis of high accumulation of various heavy metals including Cd, Zn, Cu, Pb, Fe, Cr, Co and Ni (Ji et al, 2006). Thus R. philippinarum has been not only used for heavy metal pollution monitoring, but also widely employed as a suitable bioindicator in marine toxicology Liu et al, 2013;Matozzo et al, 2004;Wang et al, 2011;Wu and Wang, 2011a;Wu et al, 2011b;Zhao et al, 2010;Zhang et al, 2012). For example, Matozzo et al (2004) reported the oxidative stresses of 4-nonylphenol in both gills and digestive gland of R. philippinarum and suggested that toxicology studies should be carried out at environmentally realistic concentrations of contaminants.…”

Section: Introductionmentioning

confidence: 99%

Toxicological evaluation of two pedigrees of clam Ruditapes philippinarum as bioindicators of heavy metal contaminants using metabolomics

Cao

2015

Environmental Toxicology and Pharmacology

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EcotoxicologyHeavy metals Metabolomics NMR a b s t r a c t Heavy metal pollution has been of great concern in the Bohai marine environment. Manila clam Ruditapes philippinarum has been used as a bioindicator in marine toxicology. In this study, NMR-based metabolomics was used to ascertain whether there were significant biological differences between two dominant pedigrees (White and Zebra) of clam and evaluate the suitability of two pedigrees for marine environmental toxicology, together with antioxidant enzymatic analysis. Our results indicated that there were significant biological differences between White and Zebra clams based on the metabolic profiles and antioxidant enzyme activities. In details, the metabolic profiles showed higher levels of amino acids and succinate in Zebra clam digestive glands and higher levels of ATP in White clam digestive glands, respectively. The superoxide dismutase activities in control White and Zebra clam samples were significantly different. Additionally, White clam was more sensitive to Cd based on the significant accumulation of Cd, antioxidant enzymatic alterations and sensitive metabolic changes. Overall, we concluded that White clam could be a preferable bioindicator for marine environmental toxicology.

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Cloning and characterization of an invertebrate type lysozyme from Venerupis philippinarum

Cited by 49 publications

References 39 publications

Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum

Cloning and characterization of allograft inflammatory factor-1 (AIF-1) from manila clam Venerupis philippinarum

Metabolic profiling of the tissue-specific responses in mussel Mytilus galloprovincialis towards Vibrio harveyi challenge

Toxicological evaluation of two pedigrees of clam Ruditapes philippinarum as bioindicators of heavy metal contaminants using metabolomics

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