Cloning and characterization of BES1/BZR1 transcription factor genes in maize

Yu, Haoqiang; Feng, Wei; Sun, Fuai; Zhang, Yuanyuan; Qu, Jingtao; Liu, Bingliang; Lu, Fengzhong; Yang, Lin; Fu, Feng; Li, Wan-Chen

doi:10.1007/s10725-018-0424-2

Cited by 53 publications

(59 citation statements)

References 72 publications

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“…Some prior studies have shown that the BES1/BZR1 genes are regulated by drought, salt, cold and heat stress in Brassica rapa, Eucalyptus grandis, Brassica napus and Arabidopsis [44][45][46], and accelerate drought, heat and freezing tolerance in tomato or Arabidopsis [32][33][34][35], implying that BES1/BZR1 genes participate in the osmotic stress response. In our study, the ZmBES1/BZR1-5 gene also responds to salt and drought stress in maize (Figure 1), which may be related to the cis-acting elements of its promoter [23]. The ZmBES1/BZR1-5 protein was likewise found to be localized to the nucleus in tobacco leaf and in transgenic Arabidopsis (Figure 2 and Figure S1B), and did not generate any defective phenotype in transgenic Arabidopsis (Figure S1A).…”

Section: Discussionmentioning

confidence: 54%

“…in response to ABA [23]. These results indicate that ZmBES1/BZR1-5 may play an important role in stress tolerance to salt, drought and ABA response.…”

Section: Int J Mol Sci 2020 21 X For Peer Review 3 Of 15mentioning

confidence: 70%

“…Moreover, the expression of ZmBES1/BZR1-5 in maize shoot and root was inhibited by PEG treatment and both reached a minimum at 3 h after treatment ( Figure 1B). In our previous study, we demonstrated that the expression of the ZmBES1/BZR1-5 gene was upregulated in maize shoots and downregulated in roots in response to ABA [23]. These results indicate that ZmBES1/BZR1-5 may play an important role in stress tolerance to salt, drought and ABA response.…”

Section: Expression Analysis Of Zmbes1/bzr1-5 Genementioning

confidence: 82%

“…BES1 shares a high identity with BZR1 at the amino acid level (88%) and N-terminal domain (97%), and several family members have been identified, including BES1/BZR1 homologs 1-4 (BEH1-4) [18,19]. These transcription factors show functional redundancy in the BR signaling transduction pathway and, hence, have been renamed BES1/BZR1s [18,19,23]. It has been demonstrated that BES1/BZR1 family members are plant-specific transcription factors and play essential roles in plant growth and development [24,25].…”

Section: Introductionmentioning

confidence: 99%

“…However, the function of ZmBES1/BZR1 genes remains unknown. In our previous study, the ZmBES1/BZR1-5 gene was characterized and showed structural and evolutionary diversity compared to other ZmBES1/BZR1 genes in maize [23]. In order to study the function of the ZmBES1/BZR1-5 gene, in this study, the expression pattern and subcellular localization of ZmBES1/BZR1-5 were analyzed.…”

Section: Introductionmentioning

confidence: 99%

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Maize ZmBES1/BZR1-5 Decreases ABA Sensitivity and Confers Tolerance to Osmotic Stress in Transgenic Arabidopsis

Sun

et al. 2020

IJMS

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The BRI1-EMS suppressor 1 (BES1)/brassinazole-resistant 1 (BZR1) transcription factors, key components in the brassinosteroid signaling pathway, play pivotal roles in plant growth and development. However, the function of BES1/BZR1 in crops during stress response remains poorly understood. In the present study, we characterized ZmBES1/BZR1-5 from maize, which was localized to the nucleus and was responsive to abscisic acid (ABA), salt and drought stresses. Heterologous expression of ZmBES1/BZR1-5 in transgenic Arabidopsis resulted in decreased ABA sensitivity, facilitated shoot growth and root development, and enhanced salt and drought tolerance with lower malondialdehyde (MDA) content and relative electrolyte leakage (REL) under osmotic stress. The RNA sequencing (RNA-seq) analysis revealed that 84 common differentially expressed genes (DEGs) were regulated by ZmBES1/BZR1-5 in transgenic Arabidopsis. Subsequently, gene ontology and KEGG pathway enrichment analyses showed that the DEGs were enriched in response to stress, secondary metabolism and metabolic pathways. Furthermore, 30 DEGs were assigned to stress response and possessed 2–15 E-box elements in their promoters, which could be potentially recognized and bound by ZmBES1/BZR1-5. Taken together, our results reveal that the ZmBES1/BZR1-5 transcription factor positively regulates salt and drought tolerance by binding to E-box to induce the expression of downstream stress-related genes. Therefore, our study contributes to the better understanding of BES1/BZR1 function in the stress response of plants.