Cloning and characterization of elongation factor 1-? of Schistosoma mansoni

Schüssler, P; Grevelding, CG; Kunz, Werner

doi:10.1007/s004360050234

Cited by 2 publications

(2 citation statements)

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“…Id induced, PT pass-through, Elu an eluate fraction. The arrow indicates purified CsEF-1α protein sequence was found in the EF-1α of C. sinensis and of S. mansoni (Schüßler et al 1997), Onchocerca volvulus (Alarcon and Donelson 1991), Trypanosoma brucei (Kaur and Ruben 1994), T. cruzi (Billaut-Mulot et al 1996), and Theileria annulata (Pain et al 2005). However, this sequence is absent from the EF-1α of three species of Plasmodium (Vinkenoog et al1998) and from the EF-1α's of Trichomonas vaginalis (Carlton et al 2007), Cryptosporidium hominis (Xu et al 2004), Giardia lamblia (McArthur et al 2000), Entamoeba histolytica (de Meester et al 1991), and of the fungus Candida albicans.…”

Section: Discussionmentioning

confidence: 99%

“…EF-1 is an abundant intracellular protein and constitutes 3-10% of cytoplasmic proteins (Slobin 1980;Billaut-Mulot et al 1996). Through its involvement in protein synthesis, EF-1 participates in the regulation of cell proliferation, senescence, motility, embryogenesis, and signal transduction (Kaur and Ruben 1994;Schüßler et al 1997;Moriya et al 2001).…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and phylogenetic analysis of Clonorchis sinensis elongation factor-1α

Kim

Cho

et al. 2007

Parasitol Res

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Elongation factor-1 (EF-1) plays a primary role in protein synthesis, e.g., in the regulation of cell growth, aging, motility, embryogenesis, and signal transduction. The authors identified a clone CsIH23 by immunoscreening a Clonorchis sinensis cDNA library. The cDNA of CsIH23 was found to have a putative open reading frame containing 461 amino acids with a predicted molecular mass of 50.5 kDa. Its polypeptide sequence was highly homologous with EF-1alpha of parasites and vertebrate animals. CsIH23 polypeptide contained three GTP/GDP-binding sites, one ribosome-binding domain, one actin-binding domain, one tRNA-binding domain, and two glyceryl-phosphoryl-ethanolamine attachment sites. Based on these primary and secondary structural similarities, it was concluded that CsIH23 cDNA encodes C. sinensis EF-1alpha (CsEF-1alpha). In a molecular phylogenic tree, CsEF-1alpha clustered with the EF-1alpha of helminthic parasites. Subsequently, CsEF-1alpha recombinant protein was bacterially overexpressed and purified by Ni-NTA affinity column chromatography. Immunoblotting using CsEF-1alpha recombinant protein produced positive signals for all serum samples tested from clonorchiasis, opisthorchiasis viverinii, and paragonimiasis westermani patients and normal healthy controls. These findings suggest that recombinant CsEF-1alpha is of limited usefulness as serodiagnostic antigen for clonorchiasis.

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