Cloning and characterization of Farnesyl pyrophosphate synthase gene from Anoectochilus

Yang, Lin; Zhang, Jun Cheng; Li, Wan Chen; Qu, Jing; Yu, Hao; Jiang, Fan; Fu, Feng

doi:10.30848/pjb2020-3(14)

Cited by 4 publications

(2 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lastly, FPP is converted into various sesquiterpenoids by TPS. In plants, FPS is a branch point enzyme in the sesquiterpenoids pathway that performs an important role in sesquiterpenoids biosynthesis [ 9 ]. Therefore, cloning and characterization of the MrFPS genes from German chamomile could be helpful for further studies on the biosynthesis and regulation of sesquiterpenoids.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Biosynthesis of α-Bisabolol by Farnesyl Diphosphate Synthase and α-Bisabolol Synthase and Their Related Transcription Factors in Matricaria recutita L.

Tai

Wang

Yao

et al. 2023

IJMS

View full text Add to dashboard Cite

The essential oil of German chamomile (Matricaria recutita L.) is widely used in food, cosmetics, and the pharmaceutical industry. α-Bisabolol is the main active substance in German chamomile. Farnesyl diphosphate synthase (FPS) and α-bisabolol synthase (BBS) are key enzymes related to the α-bisabolol biosynthesis pathway. However, little is known about the α-bisabolol biosynthesis pathway in German chamomile, especially the transcription factors (TFs) related to the regulation of α-bisabolol synthesis. In this study, we identified MrFPS and MrBBS and investigated their functions by prokaryotic expression and expression in hairy root cells of German chamomile. The results suggest that MrFPS is the key enzyme in the production of sesquiterpenoids, and MrBBS catalyzes the reaction that produces α-bisabolol. Subcellular localization analysis showed that both MrFPS and MrBBS proteins were located in the cytosol. The expression levels of both MrFPS and MrBBS were highest in the extension period of ray florets. Furthermore, we cloned and analyzed the promoters of MrFPS and MrBBS. A large number of cis-acting elements related to light responsiveness, hormone response elements, and cis-regulatory elements that serve as putative binding sites for specific TFs in response to various biotic and abiotic stresses were identified. We identified and studied TFs related to MrFPS and MrBBS, including WRKY, AP2, and MYB. Our findings reveal the biosynthesis and regulation of α-bisabolol in German chamomile and provide novel insights for the production of α-bisabolol using synthetic biology methods.

show abstract

Section: Discussionmentioning

confidence: 99%

“…FPS genes have been identified in various plant species. Yang et al [ 9 ] cloned and analyzed FPS genes from Anoectochilus roxburghii and Anoectochilus formosanus . Gaffe et al [ 10 ] found that FPS played an important role in the early development (cell division and enlargement) of plant organs in tomato.…”

Section: Introductionmentioning

confidence: 99%