Cloning and characterization ofmutLandmutSgenes ofVibrio cholerae: nucleotide sequence of themutLgene

Bera, Tapan K.; Ghosh, Subrata; Das, Jyotirmoy

doi:10.1093/nar/17.15.6241

Cited by 13 publications

(5 citation statements)

References 31 publications

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“…In the exponential phase, the V. cholerae wild-type and vchM mutant strains had comparable spontaneous mutation frequencies (Table 2), indicating that T : G mismatches arising from deamination of m5C in the parent strain were efficiently repaired. This result was consistent with reports that an efficient MMR repair system is present in V. cholerae (Bera et al, 1989). In the stationary phase of growth, the spontaneous mutation frequency of the parent strain was significantly higher than that of the corresponding vchM mutant strain CM319 ( Table 2).…”

Section: Spontaneous Mutation Frequencysupporting

confidence: 92%

“…DNA adenine methylation has been reported in V. cholerae, and a role of the V. cholerae DNA adenine methyltransferase (Dam) in virulence (Julio et al, 2001) and chromosome replication (Egan & Waldor, 2003) has been demonstrated. Although V. cholerae possesses an efficient Dam-directed mismatch repair pathway (Bera et al, 1989), it lacks homologues of Escherichia coli Dcm and Vsr endonuclease (Bhakat et al, 1999), known to be associated with the very short patch (VSP) repair system. An examination of the V. cholerae strain N16961 genome sequence database (www.tigr.org) revealed the presence of a single ORF annotated as a putative cytosine MTase.…”

Section: Introductionmentioning

confidence: 99%

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An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae

Banerjee

Chowdhury

2006

Microbiology

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5-Methyl cytosine (m5C) was detected in genomic DNA of the enteric pathogen Vibrio cholerae by HPLC analysis and immunoblotting with m5C-specific antibody. Although cleavage with the restriction endonuclease EcoRII revealed the absence of a Dcm homologue in V. cholerae, analysis of the genome sequence indicated the presence of a gene, designated in this study as vchM, which encodes a DNA (cytosine-5-)-methyltransferase (m5C-MTase) designated M.Vch. M.Vch is not associated with a restriction endonuclease or a mismatch very short patch repair (Vsr)-like endonuclease and is hence an 'orphan' or solitary MTase, although analysis of a phylogenetic tree indicated that related cytosine MTases are all components of restriction-modification systems. M.Vch recognizes and methylates the first 59 C in the degenerate sequence 59-RCCGGY-39. RT-PCR analysis suggested that vchM gene expression is increased during the stationary phase of growth. During stationary phase, the spontaneous mutation frequency in the V. cholerae wild-type strain was significantly higher than in the corresponding vchM mutant strain, suggesting that the presence of M.Vch and the absence of a very short patch (VSP) repair-like system imposes upon V. cholerae a mutator phenotype.

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Section: Spontaneous Mutation Frequencysupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae

Banerjee

Chowdhury

2006

Microbiology

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“…In the course of our studies on DNA mismatch repair in Vibrio cholerae, a gram-negative noninvasive enteric bacterium and the causative agent of the diarrheal disease cholera, several mutator genes involved in methyl-directed mismatch repair, mutL, mutS, and dam, have been identified (5,6). In the present report, evidence is presented to show that V. cholerae cells lack the dcm methyltransferase gene and the dcm-vsrmediated VSP repair mechanism.…”

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confidence: 65%

The mutK Gene of Vibrio cholerae : a New Gene Involved in DNA Mismatch Repair

1999

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A new gene, mutK, of Vibrio cholerae, encoding a 19-kDa protein which is involved in repairing mismatches in DNA via a presumably methyl-independent pathway, has been identified. The product of the mutK gene cloned in either high- or low-copy-number vectors can reduce the spontaneous mutation frequency of Escherichia coli mutS, mutL,mutU, and dam mutants. The spontaneous mutation frequency of a chromosomal mutK knockout mutant was almost identical to that of wild-type V. cholerae cells, indicating that when the methyl-directed mismatch repair is blocked, the repair potential of MutK becomes apparent. The complete nucleotide sequence of the mutK gene has been determined, and the deduced amino acid sequence showed three open reading frames (ORFs), of which the ORF3 represents the mutK gene product. ThemutK gene product has no significant homology with any of the proteins deposited in the EMBL data bank. ORF2, located upstream ofmutK, encodes a 14-kDa protein which has more than 70% homology with a hypothetical protein found only downstream of theE. coli vsr gene. ORF1, located farther upstream ofmutK, has more than 80% homology with a major cold shock protein found in several bacteria. Downstream of mutK, a partial ORF having 60% homology with an RNA methyltransferase has been identified. The mutK gene has recently been positioned in the ordered cloned DNA map of the genome of the V. choleraestrain from which the gene was isolated (10).

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“…Functional properties was done according to Bera et al, 17 as modified by Odedeji et al 18 Foaming capacity and stability 2g flour sample and 50mL distilled water were mixed in a blender at room temperature. The suspension was stirred for 5minutes at 1000rpm.…”

Section: Functional Propertiesmentioning

confidence: 99%

“…It was left at room temperature for 30minutes. And the volume of foam recorded were determined according to Bera et al, 17 and as modified by Odedeji & Oyeleke. 18 The percentage increase in volume after 30 sec was expressed as foaming capacity.…”

Section: Functional Propertiesmentioning

confidence: 99%

Effect of partial replacement of fat in sausage diet with different flours at 5%, 10% and 15% level of inclusion on performance, hematological and serum biochemistry of wistar albino rat

Oshibanjo¹,

Akinfolarin²,

Adesope³

et al. 2020

AOWMC

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The demand for functional meats with reduced Animal Fat (AF) has increased globally. Chronic health disorders are often associated with high levels of dietary AF in meat products such as beef sausage (BS). Functional beef sausage (FBS) could be produced with alternative flours as replacer for AF. Effect of partial replacement of fat in sausage diet with different flours at 5%, 10% and 15% level of inclusion on performance, hematological and serum biochemistry of Wister albino rat was evaluated. The BS (g/100g: beef 65.0, lard 20.0, sodium chloride (SC) 2.0, others 13.0) was used. The FBS were developed using Wheat flour (WF), Irish potato flour (IPF) or corn flour (CF) at 5%, 10% and 15% to replace fat (lard) in beef sausage recipe. Functional properties of the flour were carried out using standard procedure. 60 weaned male albino rats (Wister strain) weighing between 60 - 62g were purchased. The rats were allotted to 12 dietary treatments with 6 rats per treatment in a completely randomised design. The study lasted 21 days and changes in their body weight were recorded. Feed Intake (FI), Feed Conversion Ratio (FCR), Protein Intake (PI) and Protein Efficiency Ratio (PER) were calculated. At the end of the feeding period, the animals were fasted of feed for 12 hours before blood samples were collected for hematological and serum biochemical analysis. Data were analysed using descriptive statistics and ANOVA at α0.05.Irish potato flour was significantly higher in bulk density, oil retention, foaming capacity, oil absorption capacity and emulsion activities compared to other flours. Corn flour was statistically higher (P<0.05) in water retention, foam stability and water absorption capacity. Significant differences (P<0.05) were observed in all the hematological indices except for red blood cell which had similar values (P>0.05). However, there were no consistent trend observed in white blood cell count and its differential counts, which were rather fluctuating. Rats fed Irish potato based sausage had the highest Alanine Amino transferase (ALT) value while rats fed wheat based sausage diet had the lowest ALT of 11.87i.u/L. The Aspartate Aminotransferase (AST) assay of rats reveals that rats fed casein-based diet and lard-based sausage had the highest AST values of 7.30i.u/L each with least AST in rats fed protein deficient diet. The urea value for rats on the casein-based diet was the highest (45.29 mg/dL) with least value in rats fed corn-based sausage diet. Rats fed wheat-based sausage had the highest cholesterol (107.97mg/dL), while corn-based sausage had the lowest cholesterol value of 56.16 mg/dL. Corn based breakfast sausage diet was higher (P<0.05) in protein efficiency ratio with least score in protein deficient diet. Corn-based sausage had the highest biological value (95.68) with protein deficient diet having the least value (15.49).It was observed that rats fed corn-based breakfast sausage had the highest gained weight of 27g, while rats fed non-protein diet had the lowest weight change of -11g. Replacement of animal fat with corn flour at 10% enhanced better growth performance, hematological and serum biochemistry.

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Cloning and characterization ofmutLandmutSgenes ofVibrio cholerae: nucleotide sequence of themutLgene

Cited by 13 publications

References 31 publications

An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae

An orphan DNA (cytosine-5-)-methyltransferase in Vibrio cholerae

The mutK Gene of Vibrio cholerae : a New Gene Involved in DNA Mismatch Repair

Effect of partial replacement of fat in sausage diet with different flours at 5%, 10% and 15% level of inclusion on performance, hematological and serum biochemistry of wistar albino rat

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