Cloning and characterization of Somatic Embryogenesis Receptor Kinase I (EgSERK I) and its association with callus initiation in oil palm

Lee, Fong-Chin; Ong‐Abdullah, Meilina; Ooi, Siew–Eng; Ho, Chai Ling; Namasivayam, Parameswari

doi:10.1007/s11627-018-9942-x

Cited by 8 publications

(4 citation statements)

References 38 publications

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“…SERK overexpression is documented to improve the efficiency of somatic embryogenesis whereas its down-regulation has a negative impact (Hu et al, 2005). In a recent study, it has been reported that the SERK gene has a role in the somatic embryogenesis of the oil palm by triggering cellular reprogramming for the formation of callus (Lee et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

A robust workflow for indirect somatic embryogenesis and cormlet production in saffron (Crocus sativus L.) and its wild allies; C. caspius and C. speciosus

Taheri-Dehkordi

Naderi

Martinelli

et al. 2020

Heliyon

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Saffron (Crocus sativus L.) and its wild relatives, Crocus caspius and Crocus speciosus are of considerable significance in the pharmaceutical, nutraceutical, and ornamental bulbs industry. Towards the ultimate goal of the conservation of wild Crocus species and establishment of an efficient workflow for in vitro production of Crocuses, efficient protocols were developed for disinfection and in vitro production of cormlets in C. sativus and its wild allies C. caspius and C. speciosus. Moreover, the differential expression of the Somatic Embryogenesis Receptor-like Kinase (SERK) gene was evaluated as a potential molecular marker during embryogenesis between embryogenic and non-embryogenic calli. A highly efficient disinfection recipe and a low-cost TDZ-free protocol have been successfully developed for in vitro cormlet production in three Crocus species. MS medium containing 10.18 μM 2, 4-D þ 4.44 μM BAP was most efficiently induced callus and somatic embryo formation. The highest conversion frequency and maximum cormlet weight were achieved in MS containing 5.37 μM NAA þ 8.88 μM BAP. The SERK expression was significantly much higher in embryogenic calli than non-embryogenic in all Crocus species. The current low-cost and easy-to-use recipe suggests a promising in vitro propagation workflow for mass production of uniform pathogen-free cormlets of Crocus species, as well as a platform to better conservation of wild Crocus species and effective gene and genome editing using CRISPR-Cas9 in future studies.

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Section: Discussionmentioning

confidence: 99%

A robust workflow for indirect somatic embryogenesis and cormlet production in saffron (Crocus sativus L.) and its wild allies; C. caspius and C. speciosus

Taheri-Dehkordi

Naderi

Martinelli

et al. 2020

Heliyon

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“…Obtaining more number of oil palm transgenics is difficult. For achieving high regeneration efficiency in base editing technology, oil palm plants transformed with EgSERK1 (Lee et al 2019) can be used as a mother plants for base editing technology.…”

Section: The Complexities In a Polyploid Genome And Challenges Aheadmentioning

confidence: 99%

CRISPR/Cas mediated base editing: a practical approach for genome editing in oil palm

et al. 2020

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The improvement of the yield and quality of oil palm via precise genome editing has been indispensable goal for oil palm breeders. Genome editing via the CRISPR/Cas9 (CRISPR-associated protein 9) system, ZFN (zinc finger nucleases) and TALEN (transcription activator-like effector nucleases) has flourished as an efficient technology for precise target modifications in the genomes of various crops. Among the genome editing technologies, base editing approach has emerged as novel technology that could generate single base changes i.e. irreversible conversion of one target base in to other in a programmable manner. A base editor (adenine or cytosine) is a fusion of catalytically inactive CRISPR-Cas9 domain (Cas9 variants) and cytosine or adenosine deaminase domain that introduces desired point mutations. However, till date no such genetic modifications have ever been developed in oil palm via base editing technology. Precise genome editing via base editing approach can be a challenging task in oil palm due to its complex genome as well as difficulties in tissue culture and genetic transformation methods. However, availability of whole genome sequencing data in oil palm provides a platform for developing the base editing technology. Here, we briefly review the potential application and future implications of base editing technology for the genetic improvement of oil palm Keywords CRISPR/Cas • Base editors • Oil palm

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“…Alternatively, somatic embryogenesis offers an efficient and attractive approach for the micropropagation of oil palm. Somatic embryogenesis is the best choice for generating uniform clones with desirable elite and true-to-type oil palm plantlet characteristics [14,15]. Moreover, the produced clones have the ability to produce a higher oil content than the commercial seedlings [14,16].…”

Section: Introductionmentioning

confidence: 99%

“…Somatic embryogenesis is the best choice for generating uniform clones with desirable elite and true-to-type oil palm plantlet characteristics [14,15]. Moreover, the produced clones have the ability to produce a higher oil content than the commercial seedlings [14,16]. Recent reports clearly demonstrated that oil palm breeding has achieved significant contributions to the enhancement of oil yield, oil quality, and disease resistance [6,17,18,19].…”

Section: Introductionmentioning

confidence: 99%

Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

Yarra

Jin

Zhao

et al. 2019

IJMS

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Oil palm (Elaeis guineensis, Jacq.) is a prominent vegetable-oil-yielding crop. Cultivating high-yielding oil palm with improved traits is a pre-requisite to meet the increasing demands of palm oil consumption. However, tissue culture and biotechnological approaches can resolve these concerns. Over the past three decades, significant research has been carried out to develop tissue culture and genetic transformation protocols for oil palm. Somatic embryogenesis is an efficient platform for the micropropagation of oil palm on a large scale. In addition, various genetic transformation techniques, including microprojectile bombardment, Agrobacterium tumefaciens mediated, Polyethylene glycol mediated mediated, and DNA microinjection, have been developed by optimizing various parameters for the efficient genetic transformation of oil palm. This review mainly emphasizes the methods established for in vitro propagation and genetic transformation of oil palm. Finally, we propose the application of the genome editing tool CRISPR/Cas9 to improve the various traits in this oil yielding crop.

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Cloning and characterization of Somatic Embryogenesis Receptor Kinase I (EgSERK I) and its association with callus initiation in oil palm

Cited by 8 publications

References 38 publications

A robust workflow for indirect somatic embryogenesis and cormlet production in saffron (Crocus sativus L.) and its wild allies; C. caspius and C. speciosus

A robust workflow for indirect somatic embryogenesis and cormlet production in saffron (Crocus sativus L.) and its wild allies; C. caspius and C. speciosus

CRISPR/Cas mediated base editing: a practical approach for genome editing in oil palm

Progress in Tissue Culture and Genetic Transformation of Oil Palm: An Overview

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