Cloning and Characterization of the BRD7 Gene Promoter

Liu, Huaying; Peng, Cong; Zhou, Ming; Zhou, Jie; Shen, Shourong; Zhou, Hongbo; Xiong, Wei; Luo, Xiaomin; Peng, Shuping; Niu, Zhenxing; Ouyang, Jue; Li, Xiaoling; Li, Guiyuan

doi:10.1089/dna.2006.25.346

Cited by 7 publications

(11 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The hZip1 gene appears to lack canonical CCAAT, TATA box, and initiator elements in its GC-rich promoter region. Such promoters containing CpG islands are known to exhibit multiple transcription initiation sites that can be alternatively active (Swick et al, 1989; Geng and Johnson, 1993; Liu et al, 2006; Sandelin et al, 2007). In the case of PC-3 prostate cancer cells, our findings suggest an alternative main transcription initiation site for hZip1.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of the major zinc uptake protein hZip1 in prostate cancer cells

Makhov

Golovine

Uzzo

et al. 2009

Gene

View full text Add to dashboard Cite

AbstracthZip1 has been characterized as the major zinc uptake transporter regulating the accumulation of zinc in prostate cells. The mechanisms regulating expression of hZip1 have not been described. To explore the mechanisms of transcriptional regulation of the hZip1 gene, we determined the putative promoter sequence for hZip1 and identified the potential transcription start site within the predicted hZip1 promoter region. To further characterize the promoter region for basal hZip1 transcription, 3′ and 5′ deletion constructs and constructs with mutated binding sites for putative transcription factors were generated by PCR amplification and assessed for transcriptional activity with a luciferase reporter assay in PC-3 prostate cancer cells. The ability of the specific transcription factors to bind the hZip1 core promoter was confirmed by EMSA, Gel Supershift and ChIP assays. Our experiments identified the core promoter region responsible for constitutive expression of hZip1 and demonstrated critical roles for SP1 and CREB1 in transcriptional regulation of the hZip1 gene in prostate cancer cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Transcriptional regulation of the major zinc uptake protein hZip1 in prostate cancer cells

Makhov

Golovine

Uzzo

et al. 2009

Gene

View full text Add to dashboard Cite

show abstract

“…These data show that methods targeting the upregulation of BRD7 are promising, targeted treatment modalities for NPC. Current research has elucidated various molecular properties of BRD7, including nuclear localization sequences and promoter regulators 196,197. Further studies are needed to discover clinically applicable methods of increasing BRD7 in NPC, whether by simply increasing protein levels or upregulating expression.…”

Section: New Treatments For Npcmentioning

confidence: 99%

Nasopharyngeal carcinoma—Review of the molecular mechanisms of tumorigenesis

et al. 2008

View full text Add to dashboard Cite

Nasopharyngeal carcinoma (NPC) is a head and neck cancer rare throughout most of the world but common in certain geographic areas, such as southern Asia. While environmental factors and genetic susceptibility play important roles in NPC pathogenesis, the Epstein-Barr virus in particular has been implicated in the molecular abnormalities leading to NPC. There is upregulation of cellular proliferation pathways such as the Akt pathway, mitogen-activated protein kinases, and the Wnt pathway. Cell adhesion is compromised due to abnormal E-cadherin and β-catenin function. Aberrations in cell cycle are due to dysregulation of factors such as p16, cyclin D1, and cyclin E. Anti-apoptotic mechanisms are also upregulated. There are multiple abnormalities unique to NPC that are potential targets for novel treatments. Keywordsnasopharyngeal carcinoma (NPC); Epstein; Barr virus (EBV); LMP1; tumorigenesis; review Nasopharyngeal carcinoma (NPC) is a squamous cell carcinoma that usually develops around the ostium of the Eustachian tube in the lateral wall of the nasopharynx. 1 Though rare among whites, NPC is particularly common in the southern Chinese population of Guangdong, Inuits of Alaska, and native Greenlanders. 2,3 Chinese emigrants continue to have a high incidence of the disease, but the rate of NPC among ethnic Chinese born in North America is considerably lower than those born in China. 4 This epidemiologic evidence implies that both environmental factors and genetic susceptibility play roles in the development of NPC. The environmental factors may include exposure to nitrosamines in salted and pickled foods. 5 Certain human leukocyte antigen subtypes have been associated with NPC, as they have various genetic polymorphisms. 6 The World Health Organization classifies NPC based on histology. 7 Type 1, keratinizing squamous carcinoma, is characterized by well-differentiated cells that produce keratin. Type 2, nonkeratinizing squamous carcinoma, varies in cell differentiation but does not produce keratin. Type 3 is also nonkeratinizing, but is less differentiated, with highly variable cell types (clear cell, spindle cell, anaplastic). Types 2 and 3 NPC are Epstein-Barr virus (EBV) Correspondence to: D. M. Jablons, jablonsd@surgery.ucsf.edu. Standard treatment for NPC is radiotherapy, but concurrent adjuvant chemotherapy improves survival rates. 10 As with other cancers, the prognosis of NPC depends upon tumor size, lymph node involvement, and distant metastasis (TMN staging). 11 But NPC, in contrast to other head and neck malignancies, is highly sensitive to radiation and chemotherapy. 12,13 High survival rates are reported for stage 1 and 2 diseases, but the prognosis for metastatic disease remains poor even with combined radiation and chemotherapy treatment, with disease relapse rates as high as 82%. 14,15 Unfortunately, the majority of NPC is diagnosed at an advanced stage because of non-specific presenting symptoms (cervical nodal enlargement, headache, nasal and aural dysfunction), delay in seeking treat...

show abstract

“…BRD7 is also a subunit of the SWI/SNF complex specific for polybromo BRG1-associated factor (PBAF) [19], modulates chromatin Electronic supplementary material The online version of this article (doi:10.1007/s11010-015-2568-y) contains supplementary material, which is available to authorized users. remodeling by binding to acetylated histone H3 [20,21] to activate/inhibit some BRD7-dependent transcription [17,22], and plays a critical role as a transcription regulator in several important tumor suppressor pathways [22][23][24][25]. However, given the divergent cellular roles of the BRD7 gene, the BRD7 target gene and its downstream gene regulating network have not yet been explored.…”

Section: Introductionmentioning

confidence: 99%

Integrating ChIP-sequencing and digital gene expression profiling to identify BRD7 downstream genes and construct their regulating network

Xiong

Zhou

et al. 2015

Mol Cell Biochem

Self Cite

View full text Add to dashboard Cite

BRD7 is a single bromodomain-containing protein that functions as a subunit of the SWI/SNF chromatin-remodeling complex to regulate transcription. It also interacts with the well-known tumor suppressor protein p53 to trans-activate genes involved in cell cycle arrest. In this paper, we report an integrative analysis of genome-wide chromatin occupancy of BRD7 by chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-seq) and digital gene expression (DGE) profiling by RNA-sequencing upon the overexpression of BRD7 in human cells. We localized 156 BRD7-binding peaks representing 184 genes by ChIP-sequencing, and most of these peaks were co-localized with histone modification sites. Four novel motifs were significantly represented in these BRD7-enriched regions. Ingenuity pathway analysis revealed that 22 of these BRD7 target genes were involved in a network regulating cell death and survival. DGE profiling identified 560 up-regulated genes and 1088 down-regulated genes regulated by BRD7. Using Gene Ontology and pathway analysis, we found significant enrichment of the cell cycle and apoptosis pathway genes. For the integrative analysis of the ChIP-seq and DEG data, we constructed a regulating network of BRD7 downstream genes, and this network suggests multiple feedback regulations of the pathways. Furthermore, we validated BIRC2, BIRC3, TXN2, and NOTCH1 genes as direct, functional BRD7 targets, which were involved in the cell cycle and apoptosis pathways. These results provide a genome-wide view of chromatin occupancy and the gene regulation network of the BRD7 signaling pathway.

show abstract

Cloning and Characterization of the BRD7 Gene Promoter

Cited by 7 publications

References 29 publications

Transcriptional regulation of the major zinc uptake protein hZip1 in prostate cancer cells

Transcriptional regulation of the major zinc uptake protein hZip1 in prostate cancer cells

Nasopharyngeal carcinoma—Review of the molecular mechanisms of tumorigenesis

Integrating ChIP-sequencing and digital gene expression profiling to identify BRD7 downstream genes and construct their regulating network

Contact Info

Product

Resources

About