Cloning and characterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73

Luo, Yingchun; Glisson, John R.; Jackwood, Mark W.; Hancock, Robert E. W.; Bains, Manjeet; Cheng, I-Hsing N; Wang, Chinling

doi:10.1128/jb.179.24.7856-7864.1997

Cited by 63 publications

(60 citation statements)

References 44 publications

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“…O gene ompH, descrito por Luo et al (1997Luo et al ( , 1999, além de apresentar polimorfismo entre diferentes sorotipos somáticos, está envolvido em diferenças antigênicas entre cepas que têm sido estudadas para o desenvolvimento de vacinas mais eficientes. Foram desenhados primers em regiões conservadas entre os diferentes sorotipos, e que flanqueavam as regiões hipervariáveis de ompH.…”

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Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite

et al. 2002

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“…Outro enfoque, relacionado com a caracterização antigênica foi realizado por Luo et al (1997Luo et al ( , 1999. Estes autores caracterizaram uma porina denominada proteína H ou porina H de 15 sorotipos somáticos de P. multocida.…”

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Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite

et al. 2002

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“…Pasteurella multocida produces a trimeric porin, OmpH, that is remotely related to the enterobacterial porins in sequence (364). OmpH is similar to E. coil porins in terms of permeability on the basis of the reported single-channel conductivity (364,365). The genome of this organism contains one AcrB-like transporter, and the inactivation of tolC makes the organism susceptible to a wide range of agents (366).…”

Section: Pasteurellaceaementioning

confidence: 99%

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

2015

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SUMMARY The global emergence of multidrug-resistant Gram-negative bacteria is a growing threat to antibiotic therapy. The chromosomally encoded drug efflux mechanisms that are ubiquitous in these bacteria greatly contribute to antibiotic resistance and present a major challenge for antibiotic development. Multidrug pumps, particularly those represented by the clinically relevant AcrAB-TolC and Mex pumps of the resistance-nodulation-division (RND) superfamily, not only mediate intrinsic and acquired multidrug resistance (MDR) but also are involved in other functions, including the bacterial stress response and pathogenicity. Additionally, efflux pumps interact synergistically with other resistance mechanisms (e.g., with the outer membrane permeability barrier) to increase resistance levels. Since the discovery of RND pumps in the early 1990s, remarkable scientific and technological advances have allowed for an in-depth understanding of the structural and biochemical basis, substrate profiles, molecular regulation, and inhibition of MDR pumps. However, the development of clinically useful efflux pump inhibitors and/or new antibiotics that can bypass pump effects continues to be a challenge. Plasmid-borne efflux pump genes (including those for RND pumps) have increasingly been identified. This article highlights the recent progress obtained for organisms of clinical significance, together with methodological considerations for the characterization of MDR pumps.

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“…The heat-modifiable and porin proteins are important classes of OMPs that are surface-exposed and exhibit molecular mass and antigenic variation (Sikkema & Murphy, 1992;Duim et al, 1997). P. multocida expresses heat-modifiable (OmpA) and porin (OmpH) proteins on the cell surface (Lugtenberg et al, 1984;Luo et al, 1997Luo et al, , 1999, but very little is known about the precise roles of these proteins in pathogenesis. Heterogeneity of the OmpH protein in somatic serotype strains of P. multocida is due to the presence of hypervariable surface-exposed loop regions (Luo et al, 1999).…”

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Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

Davies

MacCorquodale

Baillie

et al. 2003

Journal of Medical Microbiology

104

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One hundred and fifty-eight porcine strains of Pasteurella multocida, recovered primarily from cases of pneumonic pasteurellosis or progressive atrophic rhinitis (PAR) in England and Wales, were characterized by determination of their capsular types, presence or absence of the toxA gene and molecular mass heterogeneity of the heat-modifiable (OmpA) and porin (OmpH) proteins. Eighteen groups (clones) of strains were identified on the basis of specific combinations of capsular type, toxA status and outer-membrane protein (OMP)-type. The data provided evidence that different subpopulations of P. multocida are responsible for pneumonia and PAR in pigs. The majority (88 %) of cases of pneumonia were associated exclusively with non-toxigenic capsular type A strains of OMP-types 1.1, 2.1, 3.1 and 5.1 and capsular type D isolates of OMP-type 6.1. These strains were recovered from widespread geographical locations within England and Wales over a 12-year period and represented mostly single sporadic cases. The association of a small number of P. multocida variants with the majority of cases of porcine pneumonia suggests that these strains are not opportunistic pathogens of low virulence but represent primary pathogens with a relatively high degree of virulence. In contrast, the majority (76 %) of cases of PAR were associated with toxAcontaining capsular type D strains of OMP-type 4.1 and capsular type A and D strains of OMP-type 6.1. Toxigenic capsular type A strains associated with PAR and non-toxigenic capsular type A strains associated with pneumonia represent distinct subpopulations of P. multocida that can be differentiated by their OMP-types. The association of capsular types A and D with strains of the same OMP-types, and the absence and presence of the toxA gene in strains of the same OMP-types, suggest that horizontal transfer of capsular biosynthesis and toxA genes has occurred between strains representing certain subpopulations of P. multocida. INTRODUCTIONPasteurella multocida is the aetiological agent of progressive atrophic rhinitis (PAR) of swine and is of considerable economic importance to the pig-rearing industry throughout the world (Chanter & Rutter, 1989). PAR is characterized by atrophy of the nasal turbinate bones which, in severe cases, can lead to facial distortion. P. multocida strains associated with PAR usually produce a 145-kDa dermonecrotic toxin, which is encoded by the toxA gene (Lax et al., 1990;Buys et al., 1990). This toxin induces osteolysis in the turbinate bones and plays an important role in the pathogenesis of PAR (Kamp & Kimman, 1988). Toxigenic strains associated with PAR are most frequently of capsular type D (Eamens et al., 1988;Foged et al., 1988;Gardner et al., 1994;Lariviere et al., 1992), although toxigenic isolates of capsular type A can also be involved in the disease (Fussing et al., 1999;Sakano et al., 1992). Non-toxigenic P. multocida strains are not usually associated with PAR and confirmation of toxin production is important for the diagnosis and control of the disea...

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Cloning and characterization of the major outer membrane protein gene (ompH) of Pasteurella multocida X-73

Cited by 63 publications

References 44 publications

Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite

Caracterização antigênica e fenotípica de cepas de Pasteurella multocida isoladas de pulmões de suínos com pneumonia e/ou pleurite

The Challenge of Efflux-Mediated Antibiotic Resistance in Gram-Negative Bacteria

Characterization and comparison of Pasteurella multocida strains associated with porcine pneumonia and atrophic rhinitis

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