Cloning and characterization of the lipase and lipase activator protein from Vibrio vulnificus CKM-1

Su, Jer Horng; Chang, Ming; Lee, Yeong Sheng; Tseng, I-Cheng; Chuang, Yin Ching

doi:10.1016/j.bbaexp.2004.01.003

Cited by 12 publications

(4 citation statements)

References 34 publications

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“…These toxic proteins include cytolysins, proteases, lipases, siderophores, exopolysaccharides, and effectors delivered via type III secretion systems (7,38,44,46,53,54). In V. tubiashii, several secreted proteins, including a low-molecular-weight ciliostatic toxin, are suspected virulence factors in shellfish larval vibriosis (37).…”

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confidence: 99%

The Extracellular Metalloprotease ofVibrio tubiashiiIs a Major Virulence Factor for Pacific Oyster (Crassostrea gigas) Larvae

Hasegawa

Lind

Boin

et al. 2008

Appl Environ Microbiol

131

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Vibrio tubiashii is a recently reemerging pathogen of larval bivalve mollusks, causing both toxigenic and invasive disease. Marine Vibrio spp. produce an array of extracellular products as potential pathogenicity factors. Culture supernatants of V. tubiashii have been shown to be toxic to oyster larvae and were reported to contain a metalloprotease and a cytolysin/hemolysin. However, the structural genes responsible for these proteins have yet to be identified, and it is uncertain which extracellular products play a role in pathogenicity. We investigated the effects of the metalloprotease and hemolysin secreted by V. tubiashii on its ability to kill Pacific oyster (Crassostrea gigas) larvae. While V. tubiashii supernatants treated with metalloprotease inhibitors severely reduced the toxicity to oyster larvae, inhibition of the hemolytic activity did not affect larval toxicity. We identified structural genes of V. tubiashii encoding a metalloprotease (vtpA) and a hemolysin (vthA). Sequence analyses revealed that VtpA shared high homology with metalloproteases from a variety of Vibrio species, while VthA showed high homology only to the cytolysin/hemolysin of Vibrio vulnificus. Compared to the wild-type strain, a VtpA mutant of V. tubiashii not only produced reduced amounts of protease but also showed decreased toxicity to C. gigas larvae. Vibrio cholerae strains carrying the vtpA or vthA gene successfully secreted the heterologous protein. Culture supernatants of V. cholerae carrying vtpA but not vthA were highly toxic to Pacific oyster larvae. Together, these results suggest that the V. tubiashii extracellular metalloprotease is important in its pathogenicity to C. gigas larvae.Vibriosis caused by marine Vibrio species is considered one of the most serious diseases of hatchery-reared oyster larvae (10,11,17,47,52). The disease is characterized by a rapid and dramatic reduction in larval motility, detached vela, and necrotic soft tissue, which lead to high mortality rates, exceeding 90% within 1 day of infection (45). Pathogenic agents that cause larval bivalve vibriosis have intermittently and severely curtailed shellfish hatchery production on the Atlantic and Pacific coasts of the United States, causing substantial losses in the industry (3, 10, 13). Vibrio tubiashii, a bacterial species first reported by Tubiash et al. (51), was identified as a causative agent of vibriosis (originally referred to as bacillary necrosis) in larval and juvenile bivalves of the hard clam (Mercenaria mercenaria) and Eastern oyster (Crassostrea virginica). Estes et al. (14) characterized a number of pathogenic and nonpathogenic bacterial strains from diseased Pacific oysters (Crassostrea gigas) at shellfish hatcheries on the Pacific coast of North America and described some of the highly pathogenic bacterial isolates as V. tubiashii.The genus Vibrio is the largest member of the family Vibrionaceae, which includes gram-negative and curved rod-shaped facultative anaerobes. The genus consists of at least 30 known species, which are w...

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confidence: 99%

The Extracellular Metalloprotease ofVibrio tubiashiiIs a Major Virulence Factor for Pacific Oyster (Crassostrea gigas) Larvae

Hasegawa

Lind

Boin

et al. 2008

Appl Environ Microbiol

131

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“…EBB-1 [102], Vibrio sp. [103], Vibrio vulnificus [104], Salinivibrio costicola DSM 8285 [98], Salinivibrio costicola NCIMB 13595 [98], Halobacillus sp. strain AR11 [49], Halobacillus trueperi RSK CAS9 [105], Virgibacillus alimentarius [106], Salicola sp.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Culturable Halophilic Bacteria Isolated from Mediterranean Solar Saltern for Extracellular Halotolerant Enzymes

Ali,

Abdel-Rahman,

Farahat

2024

Microbiol. Biotechnol. Lett.

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Halophilic bacteria are promising reservoirs for halotolerant enzymes that have gained much attention in biotechnological applications due to their remarkable activity and stability. In this study, 62 halophilic bacterial strains isolated from a solar saltern were screened for the production of various extracellular enzymes. The results revealed that 31 strains (50%) were positive for amylase production while 26 strains (41.9%) were positive for protease. Further, 22 strains (35.48%) exhibited β-glucosidase activity and only 17 (27.41%) demonstrated lipase activity. Of the investigated halophiles, ten strains growing in the presence of ≥15% NaCl (w/v) were selected and identified based on their 16S rRNA gene sequences as Halomonas meridiana, Salinivibrio costicola, Virgibacillus oceani, Virgibacillus marismortui, Marinobacter lipolyticus, Halobacillus karajensis, Salicola salis, Pseudoalteromonas shioyasakiensis, Salinicoccus amylolyticus, and Paracoccus salipaludis. Therefore, the present study highlights the diversity of the culturable halophilic bacteria in a Mediterranean solar saltern, harboring various valuable halotolerant enzymes.

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“…Different Vibrio species produce a series of extracellular products, which are potential pathogenic factors for marine animals. These toxins mainly include extracellular hemolysin, protease, lipase, siderophore, exopolysaccharide and effectors delivered via the type III secretion systems (16)(17)(18)(19)(20). Salinity can regulate the progression of diseases by affecting pathogens, hosts, or host-pathogen interactions.…”

Section: Introductionmentioning

confidence: 99%

Synergistic interaction of hyposalinity stress with Vibrio infection causes mass mortalities in oysters by inducing host microflora imbalance and immune dysregulation

Liu

Li²,

Yang³

et al. 2021

Preprint

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A sudden drop in salinity following extreme precipitation events usually causes mass mortalities of oysters exposed to pathogens in ocean environment. While how hyposalinity stress interacts with pathogens to cause mass mortality remains obscure. In this study, we performed an experiment by mimicking hyposalinity stress and pathogen infection with V. alginolyticus to investigate their synergistic effect on the mortality of infected oysters toward understanding of the interaction among environment, host, and pathogen. We showed that hyposalinity stress (10‰, 20‰ versus 30‰) did not significantly affect proliferation and virulence of V. alginolyticus, but significantly altered microbial composition and diversity in infected oysters. Microbial community profiling by 16S rRNA sequencing revealed disrupted homeostasis of digestive bacterial microbiota with increased abundance of several pathogenic bacteria, which may affect the pathogenesis in oysters. Transcriptome profiling of infected oysters revealed that a large number of genes associated with apoptosis and inflammation were significantly induced under hyposalinity, suggesting that hyposalinity stress may have triggered immune dysregulation in infected oysters. This work provides significant information in decoding mechanisms of synergistic interaction among environment factors, host genetics, and digestive microbiota, and how they contribute to pathogenesis.

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Cloning and characterization of the lipase and lipase activator protein from Vibrio vulnificus CKM-1

Cited by 12 publications

References 34 publications

The Extracellular Metalloprotease ofVibrio tubiashiiIs a Major Virulence Factor for Pacific Oyster (Crassostrea gigas) Larvae

The Extracellular Metalloprotease ofVibrio tubiashiiIs a Major Virulence Factor for Pacific Oyster (Crassostrea gigas) Larvae

Bioprospecting of Culturable Halophilic Bacteria Isolated from Mediterranean Solar Saltern for Extracellular Halotolerant Enzymes

Synergistic interaction of hyposalinity stress with Vibrio infection causes mass mortalities in oysters by inducing host microflora imbalance and immune dysregulation

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