Cloning and characterization of the trout perforin

Athanasopoulou, Sofia; Marioli, Dimitra; Mikrou, Angeliki; Papanastasiou, Anastasios D.; Zarkadis, Ioannis K.

doi:10.1016/j.fsi.2009.03.021

Cited by 29 publications

(10 citation statements)

References 27 publications

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“…Recombinant flounder FasL protein induced apoptosis in a Japanese flounder cell line, indicating that fish possess a Fas ligand system [ 100 ]. A perforin-like molecule has been identified in Japanese flounder [ 101 ], trout [ 102 ] and ginbuna [ 72 ], and perforin gene expression has been identified in lymphoid tissues. Furthermore, lytic activity of recombinant perforin protein in the presence of calcium has been detected in Japanese flounder [ 101 ].…”

Section: Function Of Fish T Cellsmentioning

confidence: 99%

T Cells in Fish

Nakanishi

Shibasaki

Matsuura

2015

Biology

134

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Cartilaginous and bony fish are the most primitive vertebrates with a thymus, and possess T cells equivalent to those in mammals. There are a number of studies in fish demonstrating that the thymus is the essential organ for development of T lymphocytes from early thymocyte progenitors to functionally competent T cells. A high number of T cells in the intestine and gills has been reported in several fish species. Involvement of CD4+ and CD8α+ T cells in allograft rejection and graft-versus-host reaction (GVHR) has been demonstrated using monoclonal antibodies. Conservation of CD4+ helper T cell functions among teleost fishes has been suggested in a number studies employing mixed leukocyte culture (MLC) and hapten/carrier effect. Alloantigen- and virus-specific cytotoxicity has also been demonstrated in ginbuna and rainbow trout. Furthermore, the important role of cell-mediated immunity rather than humoral immunity has been reported in the protection against intracellular bacterial infection. Recently, the direct antibacterial activity of CD8α+, CD4+ T-cells and sIgM+ cells in fish has been reported. In this review, we summarize the recent progress in T cell research focusing on the tissue distribution and function of fish T cells.

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Section: Function Of Fish T Cellsmentioning

confidence: 99%

T Cells in Fish

Nakanishi

Shibasaki

Matsuura

2015

Biology

134

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“…Knowledge about fish perforin genes is scarce, and these genes have only been characterized in 4 species [8,9,10,11]. Contrary to what occurs in higher vertebrates, fish species have more than 1 perforin gene in their genomes.…”

Section: Introductionmentioning

confidence: 99%

Proinflammatory Caspase A Activation and an Antiviral State Are Induced by a Zebrafish Perforin after Possible Cellular and Functional Diversification from a Myeloid Ancestor

et al. 2015

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In mammals, perforins play a central role in the granule-dependent cell death induced by natural killer T cells and cytotoxic T lymphocytes, and participate both in the defense against virus-infected and neoplastic cells and in the recognition of nonself molecules by the immune system. Little is known about fish perforin genes. We examined the zebrafish with the aim of increasing our knowledge about the role of perforins. We characterized 6 perforin genes in the zebrafish genome, and we studied them at the evolutionary level in combination with expression patterns in several tissues and cell populations, during both larval development and in the course of a viral infection. Our results suggest the specialization of different cell types in the production of perforins. Moreover, functional diversification during the evolution of these molecules could be inferred from this study. In particular, one of the genes, prf19b, which is mainly produced by myeloid cells, seemed to be involved in antiviral defense, conferring protection after an in vivo infection.

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“…PRF is a pore-forming toxin that polymerizes into the target cell membrane forming a pore [14]. In fish, the PRF gene has been characterized in rainbow trout [15], Japanese flounder [16] and carp [17]. Moreover, flounder recombinant PRF was able to lyse erythrocytes demonstrating its biological role as a poreforming protein [16].…”

Section: Introductionmentioning

confidence: 99%