Cloning and Characterization of the rpfC Gene of<i>Xanthomonas oryzae</i>pv.<i>oryzae:</i>Involvement in Exopolysaccharide Production and Virulence to Rice

Tang, Ji Liang; Feng, Jia‐Xun; Li, Q.-Q.; Wen, Huang; Zhou, Donggen; Wilson, T. J. G.; Ma, Qingjun; Daniels, Michael J.

doi:10.1094/mpmi-9-0664

Cited by 79 publications

(65 citation statements)

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“…Furthermore, in some of these bacteria a role for elements of this system in virulence has been demonstrated (25)(26)(27). Our analysis of the genome sequence of Xcc strain 8004 (28) indicates that it encodes 37 proteins with GGDEF, EAL, and HD-GYP domains.…”

Section: Discussionmentioning

confidence: 99%

RETRACTED: Cell–cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover

Ryan

Fouhy

Lucey

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

490

522

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HD-GYP is a protein domain of unknown biochemical function implicated in bacterial signaling and regulation. In the plant pathogen Xanthomonas campestris pv. campestris , the synthesis of virulence factors and dispersal of biofilms are positively controlled by a two-component signal transduction system comprising the HD-GYP domain regulatory protein RpfG and cognate sensor RpfC and by cell–cell signaling mediated by the diffusible signal molecule DSF (diffusible signal factor). The RpfG/RpfC two-component system has been implicated in DSF perception and signal transduction. Here we show that the role of RpfG is to degrade the unusual nucleotide cyclic di-GMP, an activity associated with the HD-GYP domain. Mutation of the conserved H and D residues of the isolated HD-GYP domain resulted in loss of both the enzymatic activity against cyclic di-GMP and the regulatory activity in virulence factor synthesis. Two other protein domains, GGDEF and EAL, are already implicated in the synthesis and degradation respectively of cyclic di-GMP. As with GGDEF and EAL domains, the HD-GYP domain is widely distributed in free-living bacteria and occurs in plant and animal pathogens, as well as beneficial symbionts and organisms associated with a range of environmental niches. Identification of the role of the HD-GYP domain thus increases our understanding of a signaling network whose importance to the lifestyle of diverse bacteria is now emerging.

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Section: Discussionmentioning

confidence: 99%

RETRACTED: Cell–cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover

Ryan

Fouhy

Lucey

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

490

522

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“…These Rpf/DSF signaling systems control interactions of Xanthomonas spp. with plants (4,20,28,41), the interaction of Xylella with both its plant host and insect vector (27), the production of extracellular enzyme virulence factors and antibiotic resistance mechanisms in Xanthomonas (3,15,37,40), and the formation of biofilms and adhesion in both genera (7,11,27). The relatedness of S. maltophilia to these plant pathogens prompted us to examine this organism for the presence and role of a DSF-dependent signaling system.…”

mentioning

confidence: 99%

Diffusible Signal Factor-Dependent Cell-Cell Signaling and Virulence in the Nosocomial PathogenStenotrophomonas maltophilia

et al. 2007

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“…However, with the exception of X. oryzae pv. oryzae (28,29), the role of DSF signaling has not been investigated for any of these other species. Here, we demonstrate that a DSF activity is detected in X. fastidiosa media extracts by an Xcc-based GFP reporter (Fig.…”

Section: Discussionmentioning

confidence: 99%

Cell-cell signaling controlsXylella fastidiosainteractions with both insects and plants

Newman

Almeida

Purcell

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

263

296

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Xylella fastidiosa, which causes Pierce's disease of grapevine and other important plant diseases, is a xylem-limited bacterium that depends on insect vectors for transmission. Although many studies have addressed disease symptom development and transmission of the pathogen by vectors, little is known about the bacterial mechanisms driving these processes. Recently available X. fastidiosa genomic sequences and molecular tools have provided new routes for investigation. Here, we show that a diffusible signal molecule is required for biofilm formation in the vector and for vector transmission to plants. We constructed strains of X. fastidiosa mutated in the rpfF gene and determined that they are unable to produce the signal activity. In addition, rpfF mutants are more virulent than the wild type when mechanically inoculated into plants. This signal therefore directs interaction of X. fastidiosa with both its insect vector and plant host. Interestingly, rpfF mutants can still form in planta biofilms, which differ architecturally from biofilms in insects, suggesting that biofilm architecture, rather than a passive response to the environment, is actively determined by X. fastidiosa gene expression. This article reports a cell-cell signaling requirement for vector transmission. Identification of the genes regulated by rpfF should elucidate bacterial factors involved in transmission and biofilm formation in the insect.

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Cloning and Characterization of the rpfC Gene ofXanthomonas oryzaepv.oryzae:Involvement in Exopolysaccharide Production and Virulence to Rice

Cited by 79 publications

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RETRACTED: Cell–cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover

RETRACTED: Cell–cell signaling in Xanthomonas campestris involves an HD-GYP domain protein that functions in cyclic di-GMP turnover

Diffusible Signal Factor-Dependent Cell-Cell Signaling and Virulence in the Nosocomial PathogenStenotrophomonas maltophilia

Cell-cell signaling controlsXylella fastidiosainteractions with both insects and plants

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