Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.

Sass, Philip M.; Field, Jeffrey; Nikawa, Jùn‐ichi; Toda, Takashi; Wigler, Michael

doi:10.1073/pnas.83.24.9303

Cited by 281 publications

(267 citation statements)

References 35 publications

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“…PKA itself is involved in this mechanism since basal cAMP levels are dramatically increased in strains with reduced activity of the kinase (Nikawa et al 1987a;Mbonyi et al 1990). cAMP is hydrolyzed by the low-and high-affinity phosphodiesterases, respectively, encoded by PDE1 and PDE2 (Sass et al 1986;Nikawa et al 1987b;Wilson and Tatchell 1988). The high-affinity phosphodiesterase Pde2 appears to control basal cAMP levels, which is important to prevent undesirable PKA activity during stationary phase (Park et al 2005).…”

Section: Regulation Of the Camp-pka Pathwaymentioning

confidence: 99%

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

et al. 2010

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Cells of all living organisms contain complex signal transduction networks to ensure that a wide range of physiological properties are properly adapted to the environmental conditions. The fundamental concepts and individual building blocks of these signalling networks are generally well-conserved from yeast to man; yet, the central role that growth factors and hormones play in the regulation of signalling cascades in higher eukaryotes is executed by nutrients in yeast. Several nutrient-controlled pathways, which regulate cell growth and proliferation, metabolism and stress resistance, have been defined in yeast. These pathways are integrated into a signalling network, which ensures that yeast cells enter a quiescent, resting phase (G 0 ) to survive periods of nutrient scarceness and that they rapidly resume growth and cell proliferation when nutrient conditions become favourable again. A series of well-conserved nutrient-sensory protein kinases perform key roles in this signalling network: i.e. Snf1, PKA, Tor1 and Tor2, Sch9 and Pho85-Pho80. In this review, we provide a comprehensive overview on the current understanding of the signalling processes mediated via these kinases with a particular focus on how these individual pathways converge to signalling networks that ultimately ensure the dynamic translation of extracellular nutrient signals into appropriate physiological responses.

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Section: Regulation Of the Camp-pka Pathwaymentioning

confidence: 99%

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

et al. 2010

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“…pUR2G (RAS2 ; Kataoka et al, 1984), pU70k (CAP1 ; Field et al, 1990), YCpCYR1 (Kataoka et al, 1985), YCpTPK1 (Broek et al, 1987) and YEpPDE2 (Sass et al, 1986) were all obtained from members of Michael Wigler's laboratory, Cold Spring Harbor Laboratory, NY.…”

Section: Yeast Strains and Plasmidsmentioning

confidence: 99%

The control of morphogenesis in Saccharomyces cerevisiae by Elm1 kinase is responsive to RAS/cAMP pathway activity and tryptophan availability

Garrett

1997

Molecular Microbiology

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SummaryMany fungi undergo a morphological transition to filamentous growth in response to limiting nutrient conditions. Constitutively elongated Saccharomyces cerevisiae mutants (elm) have been isolated; the ELM1 gene encodes a putative serine/threonine protein kinase. A novel allele, elm1-15, has been isolated in an S288C-derived strain, which causes a pleiotropic phenotype, including media-specific growth effects, abnormal morphology and altered stress response, in cells that are auxotrophic for tryptophan. elm1-15 trp1 cells cannot use many nitrogen sources, are sensitive to amino acid analogues, have very low general amino acid permease activity and do not accumulate trehalose. In contrast, haploid elm1-15 TRP1 cells grow well in budding form on all media, are stress resistant and overaccumulate trehalose. Several lines of evidence suggest that Elm1 acts on functions related to the RAS/cAMP pathway. Overexpression of Elm1 partially rescues the ts phenotype of cdc25 and cyr1 mutants. Deletion of ELM1 in low PKA activity mutants increased the severity of their phenotypes, and activation of Ras2 decreases the cell elongation phenotype of elm1 mutants. A 'signal integration' model for the complex relationship of Elm1 and the RAS/ cAMP pathway in controlling morphogenesis in response to nutrients is proposed.

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“…This effect of Ras underlies most, if not all of Ras-induced phenotypes in yeast (Sass et al, 1986;Smith et al, 1998;Stanhill et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Ha-rasval12 induces HSP70b transcription via the HSE/HSF1 system, but HSP70b expression is suppressed in Ha-rasval12-transformed cells

et al. 2005

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Heat shock proteins (Hsps) are overexpressed in many tumors, but are downregulated in some tumors. To check for a direct effect of Ha-Ras val12 on HSP70 transcription, we transiently expressed the oncoprotein in Rat1 fibroblasts and monitored its effect on HSP70b promoterdriven reporter gene. We show that expression of Ha-Ras val12 induced this promoter. Promoter analysis via systematic deletions and point mutations revealed that Ha-Ras val12 induces HSP70b transcription via heat shock elements (HSEs). Also, Ha-Ras val12 induction of HSEmediated transcription was dramatically reduced in HSF1À/À cells. Yet, residual effect of Ha-Ras val12 that was still measured in HSF1À/À cells suggests that some of the Ha-Ras val12 effect is Hsf1-independent. When HSF1À/À cells, stably expressing Ha-Ras val12 , were grown on soft agar only small colonies were formed suggesting a role for heat shock factor 1 (Hsf1) in Ha-Ras val12 -mediated transformation. Although Ha-ras Val12 seems to be an inducer of HSP70's expression, we found that in Ha-ras Val12-transformed fibroblasts expression of this gene is suppressed. This suppression is correlated with higher sensitivity of Ha-ras val12 -transformed cells to heat shock. We suggest that Ha-ras Val12 is involved in Hsf1 activation, thereby inducing the cellular protective response. Cells that repress this response are perhaps those that acquire the capability to further proliferate and become transformed clones.

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Cloning and characterization of the high-affinity cAMP phosphodiesterase of Saccharomyces cerevisiae.

Cited by 281 publications

References 35 publications

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae

The control of morphogenesis in Saccharomyces cerevisiae by Elm1 kinase is responsive to RAS/cAMP pathway activity and tryptophan availability

Ha-rasval12 induces HSP70b transcription via the HSE/HSF1 system, but HSP70b expression is suppressed in Ha-rasval12-transformed cells

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