Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)

Peng, Fan; Wang, Chao; Cheng, Yiran; Kang, Houyang; Fan, Xing; Li, Sha; Zhang, Haiqin; Zeng, Jian; Zhou, Yonghong; Wang, Yi

doi:10.3389/fpls.2018.01354

Cited by 36 publications

(15 citation statements)

References 51 publications

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“…The OsNRAMP1 and OsNRAMP5 iron transporters have been described as Cd influx transporters in the plasma membrane [27]. AtNRAMP6 is an intracellular transporter of metals such as Cd [28].…”

Section: Transporters Involved In CD Entry Into the Rootsmentioning

confidence: 99%

Cadmium Uptake by Wheat (Triticum aestivum L.): An Overview

Abedi

Mojiri

2020

Plants

132

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Cadmium is a toxic heavy metal that may be detected in soils and plants. Wheat, as a food consumed by 60% of the world’s population, may uptake a high quantity of Cd through its roots and translocate Cd to the shoots and grains thus posing risks to human health. Therefore, we tried to explore the journey of Cd in wheat via a review of several papers. Cadmium may reach the root cells by some transporters (such as zinc-regulated transporter/iron-regulated transporter-like protein, low-affinity calcium transporters, and natural resistance-associated macrophages), and some cation channels or Cd chelates via yellow stripe 1-like proteins. In addition, some of the effective factors regarding Cd uptake into wheat, such as pH, organic matter, cation exchange capacity (CEC), Fe and Mn oxide content, and soil texture (clay content), were investigated in this paper. Increasing Fe and Mn oxide content and clay minerals may decrease the Cd uptake by plants, whereas reducing pH and CEC may increase it. In addition, the feasibility of methods to diminish Cd accumulation in wheat was studied. Amongst agronomic approaches for decreasing the uptake of Cd by wheat, using organic amendments is most effective. Using biochar might reduce the Cd accumulation in wheat grains by up to 97.8%.

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“…The OsNRAMP1 and OsNRAMP5 iron transporters have been described as Cd influx transporters in the plasma membrane [27]. AtNRAMP6 is an intracellular transporter of metals such as Cd [28].…”

Section: Transporters Involved In CD Entry Into the Rootsmentioning

confidence: 99%

Cadmium Uptake by Wheat (Triticum aestivum L.): An Overview

Abedi

Mojiri

2020

Plants

132

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“…Transformants were selected for uracil prototrophy on yeast nitrogen base drop-out medium lacking uracil (SD-Ura) (Sigma, Missouri, USA) and by colony PCR using primer pairs speci c to each TpIRT1 homoeolog. Functional complementation assays were performed on a solid or liquid SD-Ura media as described by Peng et al (2018a) with minor modi cation for the concentration of mineral elements Cd (0 µM and 40 µM), Zn (0 µM and 4 mM), and Co (0 µM and 250 µM). For the functional complementation assays in a liquid medium, the single yeast colony was selected and grown overnight in SD-Ura liquid medium.…”

Section: Functional Complementation Assays In Saccharomyces Cerevisiaementioning

confidence: 99%

TpIRT1 is a transition metal transporter in Polish Wheat (Triticum polonicum L.) with a broad substrate specificity

Jiang¹,

Chen²,

Chai³

et al. 2021

Preprint

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Aims (1) Explore the metal substrate specificity of homologous TpIRT1A and TpIRT1B transporters from dwarf Polish wheat by expressing them in protoplast, yeast, and transgenic Arabidopsis; (2) screen polymorphic residues of IRT1 homologs from tetraploid and diploid ancestral wheat species that change the substrate specificity.Methods Two IRT1 homoeologs were isolated from A (TpIRT1A) and B (TpIRT1B) genomes of a tetraploid crop, polish wheat (Triticum polonicum). Both of them were analysed by expressing them in yeast and Arabidopsis protoplast, respectively. Then we constructed over-expressing transgenic plants of TpIRT1B for metals property analysis in Arabidopsis. We also isolated 22 IRT1 homoeologs from tetraploid and diploid ancestral wheat species and expressed them in yeast for function analysis. Results Our data highlighted the importance of TpIRT1 in the uptake and translocation of Fe, Mn, Co, and Cd with direct implications for wheat yield potential. Both TpIRT1A and TpIRT1B were located at the plasma membrane and internal vesicles in Arabidopsis protoplasts, and responsible for Cd and Co sensitivity in yeast. The over-expression of TpIRT1B in A. thaliana increased Fe, Mn, Co, and Cd concentration in its tissues and improved plant growth under Fe, Mn, and Co deficiencies, while causing more sensitivity to Cd than wild-type plant. Functional analysis of IRT1 homoeologs from tetraploid and diploid ancestral wheat species in yeast disclosed four distinct amino acid residues in TdiIRT1B (T. dicoccum) and TtuIRT1B (T. turgidum). Altogether, these results increase the knowledge of IRT1 function in a global crop, wheat.

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“…Due to similar chemical properties of essential mineral elements and not essential HMs, some of the MTs such as Natural Resistance-associated Macrophage Proteins (NRAMPs) transport them to upper plant parts. In dwarf polish wheat (T. polonicum), TpNRAMP3, a member of NRAMPs family was identified that is responsive for PM localized protein and it is expressed higher in leaf blades, roots and first note at jointing, booting and grain filling stages, respectively [21]. In detail, TpNRAMP3 was cloned and transformed into yeast and Arabidopsis.…”

Section: Hms Stressmentioning

confidence: 99%

“…Over-expression of TaZnF in A. thaliana significantly increased tolerance to heat and cold stress as well as promotes early flowering [16]. Similarly, there are numerous studied in many plant species evidenced the pivotal role of MTs in uptake, sequestration, translocation and detoxification of toxic HMs including cadmium (Cd) [17], chromium (Cr) [18], lead (Pb) [19], As [20], cobalt (Co) and zinc (Zn) [21], for more detail see section 3.…”

Section: Introductionmentioning

confidence: 99%

The Role of Membrane Transporters in Plant Growth and Development, and Abiotic Stress Tolerance

Gill¹,

Ahmar²,

Ali³

et al. 2021

Preprint

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Membrane transporters (MTs) are mainly localized at the plasma membrane (PM), tonoplast and vacuolar membrane (VM) of cells in all plant organs. Their work is to maintain the cellular homeostasis by controlling ionic movements across PM channels from roots to upper plant parts, xylem loading and remobilization of sugar molecules from photosynthesis tissues in the leaf (source) to roots, stem and seeds (sink) via phloem loading. The plant’s whole source-to-sink relationship is regulated by multiple transporting proteins in a highly sophisticated manner and driven based on different stages of plant growth and development (PG&D), and environmental changes. The MTs play a pivotal role in PG&D in terms of increased plant height, branches/tiller numbers, enhanced numbers, length and filled panicles per plant, seed yield and grain quality. Dynamic climatic changes disturbed the ionic balance (salt, drought and heavy metals) and sugar supply (cold and heat stress). Due to poor selectivity, some of the MTs also uptake toxic elements in the roots that negatively impact on PG&D, later on also exported to upper parts and then deteriorate the grain quality. As an adaptive strategy, in response to salt and HMs plants activated PM and VM localized MTs that export toxic elements into vacuole, and also translocate in the root’s tips and shoot. However, in case of drought, cold and heat stresses, MTs increased the water and sugar supply to all organs. In this review, we mainly reviewed recent literature from Arabidopsis, halophytes, and major field crops such as rice, wheat, maize and oilseed rape to argue on the global role of MTs in PG&D and abiotic stress tolerance. We also discussed the gene expression level changes and genomic variations within a species as well as within a family in response to developmental and environmental cues.

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Cloning and Characterization of TpNRAMP3, a Metal Transporter From Polish Wheat (Triticum polonicum L.)

Cited by 36 publications

References 51 publications

Cadmium Uptake by Wheat (Triticum aestivum L.): An Overview

Cadmium Uptake by Wheat (Triticum aestivum L.): An Overview

TpIRT1 is a transition metal transporter in Polish Wheat (Triticum polonicum L.) with a broad substrate specificity

The Role of Membrane Transporters in Plant Growth and Development, and Abiotic Stress Tolerance

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