Cloning and Characterization of Two cDNAs Encoding Sulfatases in the Roman Snail, Helix pomatia

Wittstock, Ute; Fischer, Morten; Svendsen, Ib; Halkier, Barbara Ann

doi:10.1080/713803591

Cited by 21 publications

(6 citation statements)

References 9 publications

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“…ROPN1 (ropporin 1), a protein kinase A-like (R2D2) protein, is associated with cAMP dependent protein kinase (PKA)/A-kinase anchoring protein (AKAP). Fujita et al reported that ROPN1 is localized to the sperm flagella (Fujita et al, 2000). Another study reported decreased expression of ROPN1 in the spermatozoa of patients with low sperm motility (Chen et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

Analysis of Long Non-Coding RNA and mRNA Expression Profiling in Immature and Mature Bovine (Bos taurus) Testes

Gao

Lai

et al. 2019

Front. Genet.

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Testis development and spermatogenesis are strictly regulated by numbers of genes and non-coding genes. However, long non-coding RNAs (lncRNAs) as key regulators in multitudinous biological processes have not been systematically identified in bovine testes during sexual maturation. In this study, we comprehensively analyzed lncRNA and mRNA expression profiling of six bovine testes at 3 days after birth and 13 months by RNA sequencing. 23,735 lncRNAs and 22,118 mRNAs were identified, in which 540 lncRNAs ( P -value < 0.05) and 3,525 mRNAs ( P -adjust < 0.05) were significantly differentially expressed (DE) between two stages. Correspondingly, the results of RT-qPCR analysis showed well correlation with the transcriptome data. Moreover, GO and KEGG enrichment analyses showed that DE genes and target genes of DE lncRNAs were enriched in spermatogenesis. Furthermore, we constructed lncRNA–gene interaction networks; consequently, 15 DE lncRNAs and 12 cis -target genes were involved. The target genes ( SPATA16 , TCF21 , ZPBP , PACRG , ATP8B3 , COMP , ACE , and OSBP2 ) were found associated with bovine sexual maturation. In addition, the expression of lncRNAs and cis -target genes was detected in bovine Leydig cells, Sertoli cells, and spermatogonia. Our study identified and analyzed lncRNAs and mRNAs in testis tissues, suggesting that lncRNAs may regulate testis development and spermatogenesis. Our findings provided new insights for further investigation of biological function in bovine lncRNA.

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Section: Discussionmentioning

confidence: 99%

Analysis of Long Non-Coding RNA and mRNA Expression Profiling in Immature and Mature Bovine (Bos taurus) Testes

Gao

Lai

et al. 2019

Front. Genet.

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“…This catalytic region of SULF1 and SULF2 display 80% identity. A post-translationally modified cysteine forms a C-formylglycine within the active sites of both enzymes[18] that is involved in hydrolytic cleavage of sulfates from the 6-O position of glucosamines in substrates[19-24]. Theoretical models of the catalytic cores of SULF1 and SULF2 can be built using 13 homologous crystallographic structures at present.…”

Section: Sulf1 and Sulf2 Domain Structure Substrate Specificity And mentioning

confidence: 99%

Heparin-Degrading Sulfatases in Hepatocellular Carcinoma: Roles in Pathogenesis and Therapy Targets

Lai

Thompson²,

Sandhu³

et al. 2008

Future Oncol.

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Hepatocellular carcinoma (HCC) is often diagnosed at an advanced stage for which there are limited treatment options. Two recently identified human heparin-degrading endosulfatases, named sulfatase 1 (SULF1) and sulfatase 2 (SULF2), have been found to be involved in liver carcinogenesis. SULF1 and SULF2 desulfate cell surface and extracellular matrix heparan sulfate proteoglycans (HSPGs) and modulate heparin-binding growth factor signaling in multiple cancers, including HCCs. SULF1 inhibits HCC tumor cell growth in vitro and in nude mice in vivo, partially through effects on gene expression mediated through histone H4 acetylation. While SULF1 is downregulated in the majority of HCC cell lines and approximately 30% of primary HCCs, SULF2 is up regulated in almost all HCC cell lines and in 60% of primary HCCs. In contrast to the tumor suppressor effect of SULF1, expression of SULF2 activates mitogen activated protein kinase (MAPK) and Akt pathways, promotes HCC cell growth in vitro and in vivo, and is associated with decreased survival of HCC patients. Targeting SULF2 or the interaction between SULF2 and SULF1 may lead to novel therapeutics for the treatment of HCCs.

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“…Sulfatases are highly proficient enzymes, with catalytic proficiencies (( k cat / K M )/ k uncat ) well above 1 × 10 13 to 1 × 10 17 M –1 for the model substrate 4-nitrophenyl sulfate 1d (Scheme ). − Despite their occurrence in eukaryotes and prokaryotes, relevance for a variety of key processes (e.g., development, − germination, resistance against toxic defense molecules, , mucin desulfatation, − or degradation of mucopolysaccharides , ) and the danger of various diseases as a result of their malfunction (e.g., lysosomal disorders , ), their mechanism has not been studied in the same detail as that of the related phosphatases.…”

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confidence: 99%

Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase

et al. 2019

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Pseudomonas aeruginosa arylsulfatase (PAS) hydrolyses sulfate and, promiscuously, phosphate monoesters. Enzyme-catalyzed sulfate transfer is crucial to a wide variety of biological processes, but detailed studies of the mechanistic contributions to its catalysis are lacking. We present linear free energy relationships (LFERs) and kinetic isotope effects (KIEs) of PAS and active site mutants that suggest a key role for leaving group (LG) stabilization. In LFERs PAS WT has a much less negative Brønsted coefficient (bl eaving group obs-Enz =-0.33) than the uncatalyzed reaction (bl eaving group obs =-1.81).This situation is diminished when cationic active site groups are exchanged for alanine. The considerable degree of bond breaking during the TS is evidenced by an 18 Obri dge KIE of 1.0088. LFER and KIE data for several active site mutants point to leaving group stabilization by active-site K375, in cooperation with H211. 15 N KIEs and the increased sensitivity to leaving group ability of the sulfatase activity in neat D2O (Dbl eaving group H-D = +0.06) suggest that the mechanism for S-Obri dge bond fission shifts, with decreasing leaving group ability, from charge compensation via Lewis acid interactions towards direct proton donation. 18 Ononbri dge KIEs indicate that the TS for PAS-catalyzed sulfate monoester hydrolysis has a significantly more associative character compared to the uncatalyzed reaction, while PAS-catalyzed phosphate monoester hydrolysis does not show this shift. This difference in enzyme-catalyzed TSs appears to be the major factor favoring specificity toward sulfate over phosphate esters by this promiscuous hydrolase, since other features are either too similar (uncatalyzed TS) or inherently favor phosphate (charge).Arylsulfatases catalyze the in vivo hydrolysis of sulfate monoesters, producing inorganic sulfate, typically removing it from a sugar or a steroid hormone. Sulfatases are highly proficient enzymes, 3 with catalytic proficiencies ((kcat /KM)/kuncat ) well above 10 13 -10 17 M -1 for the model substrate 4nitrophenyl sulfate 1d (Scheme 1). [1][2][3][4] Despite their occurrence in eukaryotes and prokaryotes, relevance for a variety of key processes (e.g. development, 5-11 germination, 12 resistance against toxic defense molecules, 13, 14 mucin desulfation, [15][16][17] or degradation of mucopolysaccharides 18,19 ) and the occurrence of various diseases as a result of their malfunction (e.g. lysosomal disorders 18, 20 ), their mechanism has not been studied in the same detail as that of the related phosphatases. Scheme 1. General reaction scheme for the PAS-catalyzed hydrolysis of aryl sulfate monoesters 1a-1l and aryl phosphate monoesters 2b-2l.The majority of sulfatases known to date are members of the alkaline phosphatase (AP) superfamily. The mechanism of transition state (TS) stabilization during enzyme-catalyzed substrate hydrolysis of one member of this superfamily, Escherichia coli alkaline phosphatase (EcAP), has been subject of a large number of in-depth studies involving the e...

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Cloning and Characterization of Two cDNAs Encoding Sulfatases in the Roman Snail, Helix pomatia

Cited by 21 publications

References 9 publications

Analysis of Long Non-Coding RNA and mRNA Expression Profiling in Immature and Mature Bovine (Bos taurus) Testes

Analysis of Long Non-Coding RNA and mRNA Expression Profiling in Immature and Mature Bovine (Bos taurus) Testes

Heparin-Degrading Sulfatases in Hepatocellular Carcinoma: Roles in Pathogenesis and Therapy Targets

Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase

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