Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus

Kumar, Santosh; Jaggi, Monika; Taneja, Jyoti; Sinha, Alok

doi:10.1016/j.plaphy.2011.01.012

Cited by 18 publications

(7 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of more than one POD enzyme is common in plants (Kumar et al 2011). The hypothesis for that are a gene family as well as post-transcriptional and posttranslational modifications of peroxidase transcripts give rise to multiple isozymes (Tognolli et al 2002).…”

Section: Resultsmentioning

confidence: 99%

Kinetic Characterization and Thermal Properties of Two Acidic Peroxidases from White Cabbage (Brassica OleraceaL.)

Pellicer

Lucas-Abellán

Serrano-Martínez

et al. 2016

Journal of Food Biochemistry

View full text Add to dashboard Cite

Soluble and membrane-bound peroxidases (POD) were extracted from white cabbage. Optimum activity was obtained at pH 4.0 for both enzymes. The K M and V m values for H 2 O 2 were found to be 0.62 mM and 6.5 lM/min, respectively, for soluble POD; and 1.1 mM and 7.75 lM/min, respectively, for membrane-bound POD. The K M and V m values for ABTS were found to be 0.92 mM and 7.4 lM/ min, respectively, for soluble POD; and 0.43 mM and 6.95 lM/min, respectively, for membrane-bound POD. The effect of several reducing agents was studied. The effect of cyclodextrins was studied and the complexation constant between ABTS and HP-b-CDs was calculated (K c 5 303 M 21). A thermal inactivation study showed a first-order inactivation kinetic for both enzymes and the Arrhenius plot yielded a straight line with a slope equivalent to activation energy of 153.1 kj/mol for membrane-bound POD and 49.1 kj/mol for soluble POD. PRACTICAL APPLICATIONSRoots of horseradish serve at present as the major source of commercially available peroxidase. However, this peroxidase has certain problems with regard stability and inactivation under certain conditions, hence the interest in finding novel plant peroxidases with greater stability and similar applicability. White cabbage peroxidase could be highly promising for biotechnological applications, taking into account, its high activity, low cost of the raw material and the easy extraction method described.

show abstract

Section: Resultsmentioning

confidence: 99%

Kinetic Characterization and Thermal Properties of Two Acidic Peroxidases from White Cabbage (Brassica OleraceaL.)

Pellicer

Lucas-Abellán

Serrano-Martínez

et al. 2016

Journal of Food Biochemistry

View full text Add to dashboard Cite

show abstract

“…Ritter et al (1993) demonstrated a peroxidase mRNA was induced 3fold in the young leaves of saltstressed cotton plants. Kumar et al (2011) indicated that the expression levels of CrPrx3 and CrPrx4, two new class III POD genes, were downregulated in Catharanthus roseus under salt stress. In addition, the overexpression of the sweetpotato (Ipomoea batatas) swpa4 peroxidase significantly enhanced the salt stress tolerance of tobacco plants [Nicotiana tabacum (Kim et al, 2008)].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of a Class III Peroxidase cDNA from Cucumber under Salt Stress

Fan

Chen

Zhou

et al. 2014

J. Amer. Soc. Hort. Sci.

View full text Add to dashboard Cite

Salt stress reduces the fresh weight, dry weight, and relative growth rate of cucumber (Cucumis sativus) seedlings and results in serious quality loss in cucumber production. Our previous study indicated that the netting-associated peroxidase (CsaNAPOD) protein in cucumber seedling roots was induced by salt stress. Here, we amplified the coding sequence of CsaNAPOD from a cDNA isolated from the roots of cucumber seedlings. Sequence analysis indicated that the coding sequence of CsaNAPOD is 1035 bp, encoding a deduced protein of 344 amino acids, with a predicated molecular weight of 37.2 kD and theoretical isoelectric point of 5.64. The deduced amino acid sequence of CsaNAPOD showed high sequence similarity to peroxidases (PODs) from other plant species. Moreover, CsaNAPOD possesses the typical sequence structures of class III PODs and indicated that CsaNAPOD belongs to this subfamily. CsaNAPOD was highly expressed in the roots and was weakly expressed in the stems and leaves of cucumber seedlings. Salt stress significantly increased the expression of CsaNAPOD in the leaves during the entire experimental period compared with the control, and the expression of CsaNAPOD in roots was reduced at 6 hours and induced at 48 and 72 hours by salt treatment. In stems, the expression of CsaNAPOD declined at 48 and 72 hours as a result of the salt treatment compared with the control. These results indicate that the expression of CsaNAPOD responded to salt stress in cucumber seedlings, and the expression patterns under salt stress in different tissues were not identical. Our research suggests that CsaNAPOD may have potential function during the plant response to salt stress.

show abstract

“…Many of the structural and regulatory genes, enzymes and intermediates that determine the pathway and various cell types of different organs in which specific steps of pathway are accomplished have been identified and characterized [15,20,50,67]. Processes involved in the biosynthesis of V, C, VB, and VC in leaves are understood in some detail [5,10,22,23,37,38,42,44,46,56,57,66,75]. Strictosidine, is synthesized in epidermal cells, serves as a precursor for V and C synthesis in leaf [2,18,23,29,48,49,57,66,76].…”

Section: Introductionmentioning

confidence: 99%

Detection and Mapping of QTLs Affecting Contents of Pharmaceutical Alkaloids in Leaf and Root of Catharanthus roseus

et al. 2013

View full text Add to dashboard Cite

Catharanthus roseus leaves and roots hyper-accumulate the terpenoid indole alkaloids, which are required for the economic production of pharmaceutical molecules vindoline (V), catharanthine (C), vinblastine (VB), vincristine (VC), ajmalicine (A) and serpentine (S). For developing marker-assisted selection (MAS) breeding in C. roseus, the objectives of the present study were: (a) To characterize variability and associations between six traits-V (VL), C (CL), VB ? VC (VBL) contents of leaves and C (CR), A (AR) and S (SR) contents in roots, in a recombinant inbred line (RIL) population in two environments. (b) To identify and place quantitative trait loci (QTLs) associated with the traits on the map. The population of 191 RILs was found to segregate transgressively for all the six traits. The intra-organ correlations of leaf and root traits were positive and significant. The inter-organ traits were not correlated. All the traits except AR were highly heritable (h 2 = 41-79 %). A RIL population genetic map of 179 markers on 8 linkage groups (LGs) covering 1,786.5 cM genetic distance was constructed. The RIL-generated genetic map led to identification of 20 QTLs for 6 traits (5, 4, 1, 4, 2, and 4 QTLs, respectively for CL, VL, VBL, CR, AR, and SR) by the use of single marker regression, simple interval mapping and composite interval mapping approaches. QTLs were detected only on 4 of the 8 LGs. QTL for the same or different traits were mapped in clusters or singularly. The results suggest that a combination of MAS and phenotypic selection offer possibilities of realizing simultaneous improvement in C and V accumulation in leaves and roots of C. roseus.

show abstract

Cloning and characterization of two new Class III peroxidase genes from Catharanthus roseus

Cited by 18 publications

References 42 publications

Kinetic Characterization and Thermal Properties of Two Acidic Peroxidases from White Cabbage (Brassica OleraceaL.)

Kinetic Characterization and Thermal Properties of Two Acidic Peroxidases from White Cabbage (Brassica OleraceaL.)

Isolation and Characterization of a Class III Peroxidase cDNA from Cucumber under Salt Stress

Detection and Mapping of QTLs Affecting Contents of Pharmaceutical Alkaloids in Leaf and Root of Catharanthus roseus

Contact Info

Product

Resources

About