Cloning and Characterization of α-Methylacyl Coenzyme A Racemase from
            <i>Gordonia polyisoprenivorans</i>
            VH2

Arenskötter, Quyen; Heller, Jens; Dietz, David M.; Arenskötter, Matthias; Steinbüchel, Alexander

doi:10.1128/aem.01491-08

Cited by 11 publications

(13 citation statements)

References 29 publications

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“…Another transposon-induced mutant, which could no longer grow on IR, showed the involvement of an Mce cluster that seems to be involved in the transport of the oligomers generated outside the cell. Our proposal of a further degradation of the cleavage products similar to the degradation of fatty acids is consistent with mutants of strain VH2 generated in the past (5,9) and is supported by analysis of the genome sequence. The genome harbors an extensive number of putative genes involved in ␤-oxidation.…”

Section: Resultssupporting

confidence: 65%

“…The next step is most likely catalyzed by one of the two ␣-methylacyl-CoA racemases (GPOL_c25180, GPOL_c36450) located in the genome. In previous studies, one Mcr (GPOL_c36450) was found to be essential for strain VH2, since a disruption led to a total loss of the ability to utilize rubber for growth (5). This Mcr catalyzes the conversion of the (R)-into the (S)-stereoisomer.…”

Section: Resultsmentioning

confidence: 99%

“…The proposed pathway releases propionyl-CoA, which has to be further metabolized, in addition to acetyl-CoA. Strain VH2 grows on propionate as the sole source of carbon and energy (5). Its genome possesses genes encoding a methylcitrate synthase (GPOL_c17040), an aconitate hydratase (GPOL_c23340), a methylcitrate dehydratase (GPOL_c17020), and a methylisocitrate lyase (GPOL_c17030).…”

Section: Resultsmentioning

confidence: 99%

“…However, an acetyl-CoA acetyltransferase (GPOL_c42830) is located next to these genes. The expression of this gene could be affected due to polar effects and could therefore show impaired growth on MSM-IR sandwich agar plates (5).…”

Section: Resultsmentioning

confidence: 99%

“…In the same studies, the involvement of an ␣-methylacyl-coenzyme A (CoA) racemase (Mcr) during rubber degradation was shown. Mutants with a disruption of the mcr VH2 gene lost the ability to metabolize poly-(cis-1,4-isoprene) and related methyl-branched isoprenoid compounds (5,9). Involvement of an extracellular superoxide dismutase (SodA) in rubber degradation was also demonstrated in this actinomycete (82).…”

mentioning

confidence: 88%

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Involvement of Two Latex-Clearing Proteins during Rubber Degradation and Insights into the Subsequent Degradation Pathway Revealed by the Genome Sequence of Gordonia polyisoprenivorans Strain VH2

Hiessl

Schuldes

Thürmer

et al. 2012

Appl Environ Microbiol

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ABSTRACTThe increasing production of synthetic and natural poly(cis-1,4-isoprene) rubber leads to huge challenges in waste management. Only a few bacteria are known to degrade rubber, and little is known about the mechanism of microbial rubber degradation. The genome ofGordonia polyisoprenivoransstrain VH2, which is one of the most effective rubber-degrading bacteria, was sequenced and annotated to elucidate the degradation pathway and other features of this actinomycete. The genome consists of a circular chromosome of 5,669,805 bp and a circular plasmid of 174,494 bp with average GC contents of 67.0% and 65.7%, respectively. It contains 5,110 putative protein-coding sequences, including many candidate genes responsible for rubber degradation and other biotechnically relevant pathways. Furthermore, we detected two homologues of a latex-clearing protein, which is supposed to be a key enzyme in rubber degradation. The deletion of these two genes for the first time revealed clear evidence that latex-clearing protein is essential for the microbial utilization of rubber. Based on the genome sequence, we predict a pathway for the microbial degradation of rubber which is supported by previous and current data on transposon mutagenesis, deletion mutants, applied comparative genomics, and literature search.

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Section: Resultssupporting

confidence: 65%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 88%

See 3 more Smart Citations

Involvement of Two Latex-Clearing Proteins during Rubber Degradation and Insights into the Subsequent Degradation Pathway Revealed by the Genome Sequence of Gordonia polyisoprenivorans Strain VH2

Hiessl

Schuldes

Thürmer

et al. 2012

Appl Environ Microbiol

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Actinobacteria

Kämpfer

2010

Handbook of Hydrocarbon and Lipid Microbiology

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Oligo(cis-1,4-isoprene) aldehyde-oxidizing dehydrogenases of the rubber-degrading bacterium Gordonia polyisoprenivorans VH2

Vivod

Oetermann

Hiessl

et al. 2017

Appl Microbiol Biotechnol

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The actinomycete Gordonia polyisoprenivorans strain VH2 is well-known for its ability to efficiently degrade and catabolize natural rubber [poly(cis-1,4-isoprene)]. Recently, a pathway for the catabolism of rubber by strain VH2 was postulated based on genomic data and the analysis of mutants (Hiessl et al. in Appl Environ Microbiol 78:2874-2887, 2012). To further elucidate the degradation pathway of poly(cis-1,4-isoprene), 2-dimensional-polyacrylamide gel electrophoresis was performed. The analysis of the identified protein spots by matrix-assisted laser desorption/ionization-time of flight tandem mass spectrometry confirmed the postulated intracellular pathway suggesting a degradation of rubber via β-oxidation. In addition, other valuable information on rubber catabolism of G. polyisoprenivorans strain VH2 (e.g. oxidative stress response) was provided. Identified proteins, which were more abundant in cells grown with rubber than in cells grown with propionate, implied a putative long-chain acyl-CoA-dehydrogenase, a 3-ketoacyl-CoA-thiolase, and an aldehyde dehydrogenase. The amino acid sequence of the latter showed a high similarity towards geranial dehydrogenases. The expression of the corresponding gene was upregulated > 10-fold under poly(cis-1,4-isoprene)-degrading conditions. The putative geranial dehydrogenase and a homolog were purified and used for enzyme assays. Deletion mutants for five aldehyde dehydrogenases were generated, and growth with poly(cis-1,4-isoprene) was investigated. While none of the mutants had an altered phenotype regarding growth with poly(cis-1,4-isoprene) as sole carbon and energy source, purified aldehyde dehydrogenases were able to catalyze the oxidation of oligoisoprene aldehydes indicating an involvement in rubber degradation.

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Cloning and Characterization of α-Methylacyl Coenzyme A Racemase from Gordonia polyisoprenivorans VH2

Cited by 11 publications

References 29 publications

Involvement of Two Latex-Clearing Proteins during Rubber Degradation and Insights into the Subsequent Degradation Pathway Revealed by the Genome Sequence of Gordonia polyisoprenivorans Strain VH2

Involvement of Two Latex-Clearing Proteins during Rubber Degradation and Insights into the Subsequent Degradation Pathway Revealed by the Genome Sequence of Gordonia polyisoprenivorans Strain VH2

Actinobacteria

Oligo(cis-1,4-isoprene) aldehyde-oxidizing dehydrogenases of the rubber-degrading bacterium Gordonia polyisoprenivorans VH2

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