Cloning and Chromosomal Assignment of the Porcine Interleukin-2 Receptor Alpha(IL-2R.ALPHA.) Gene.

Kokuho, Takehiro; Hiraiwa, Hideki; Yasue, Hiroshi; Watanabe, Satoko; Yokomizo, Yuichi; Inumaru, Shigeki

doi:10.1292/jvms.62.841

Cited by 5 publications

(5 citation statements)

References 33 publications

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“…In order to determine the regional correspondence between the distal half of SSC10q and HSA10p, in which VIM, GAD2, MRC1, and IL2RA were localized (Goureau et al, 1996;Kokuho et al, 2000;Pinton et al, 2000), swine BAC clones containing at least parts of VIM, GAD2, and MRC1 were first identified in the library. Two DNA fragments were produced from the BAC believed to contain VIM (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, given the discrepancy in the VIM assignment and that FISH did not establish locus order for all genes mapped, we attempted to assign VIM, GAD2, MRC1 as well as Kokuho et al (2000). b Since no markers except for SWR67 showed linkage with IL2RA at a lodscore greater than 4.8, SWR67 was tentatively placed in the proximal marker column.…”

Section: Resultsmentioning

confidence: 99%

“…b Since no markers except for SWR67 showed linkage with IL2RA at a lodscore greater than 4.8, SWR67 was tentatively placed in the proximal marker column. score score marker IL2RA, known to reside on SSC10qter (Kokuho et al, 2000), to the IMpRH map (Hawken et al, 1999). The RH mapping results are summarized in Table 2 and schematically presented in Fig.…”

Section: Resultsmentioning

confidence: 99%

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Alignment of VIM, MRC1, GAD2, and IL2RA genes on swine chromosome 10q by in situ hybridization and RH mapping

Hiraiwa

Uenishi²,

Yasue³

2001

Cytogenet Genome Res

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Since the distal half of swine chromosome (SSC) 10q was shown to contain a quantitative trait locus (QTL) influencing swine growth, the precise correspondence between this chromosome region and the orthologous human chromosome region (HSA10p) was investigated using chromosomal fluorescence in situ hybridization and RH mapping of type I loci spanning the growth QTL. The goal was to align this critical region of swine with the corresponding region in human for the purpose of identifying candidate genes. The HSA10p type I loci mapped in swine were VIM, MRC1, GAD2, and IL2RA. Locus order on SSC10q was shown to be centromere–VIM–MRC1–GAD2–IL2RA, while in human the order is centromere–GAD2–MRC1–VIM–IL2RA, indicating that the chromosome segment marked by VIM, MRC1 and GAD2 has been inverted relative to the centromere and IL2RA.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Alignment of VIM, MRC1, GAD2, and IL2RA genes on swine chromosome 10q by in situ hybridization and RH mapping

Hiraiwa

Uenishi²,

Yasue³

2001

Cytogenet Genome Res

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“…In addition, IL-12 induces STAT4 phosphorylation through activation of the mitogen-activated protein kinase kinase 6/p38 pathway (49). This pathway has recently been implicated as being important in IL-12-mediated increases in CD25 expression on CD4 T cells (28), and two STAT4-binding motifs upstream of CD25 were recently found in porcine PBMCs (50). Thus, IL-12 may contribute to up-regulation of CD25 expression by more than one signaling pathway.…”

Section: Discussionmentioning

confidence: 98%

The Roles of IL-12 in Providing a Third Signal for Clonal Expansion of Naive CD8 T Cells

Valenzuela

Schmidt

Mescher

2002

The Journal of Immunology

178

184

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Stimulation of an effective in vitro or in vivo response by naive CD8 T cells requires three signals: TCR engagement, costimulation/IL-2, and a third signal that can be provided by IL-12. In addition to being required for acquisition of cytolytic function, IL-12 is required for optimal IL-2-dependent proliferation and clonal expansion. In experiments examining in vitro stimulation of naive CD8 T cells, IL-12 is shown to stimulate expression of the IL-2R α-chain (CD25) to much higher levels than are reached in response to just TCR and costimulation and/or IL-2. In addition, high CD25 expression is substantially prolonged in the presence of IL-12. As a consequence, the cells proliferate more effectively in response to low levels of IL-2. Examination of adoptively transferred TCR transgenic CD8 T cells responding to peptide Ag confirmed that IL-12 up-regulates CD25 in vivo, even when B7-mediated costimulation is largely blocked. TCR- and IL-2-dependent proliferation of CD8 T cells from mice deficient in CD25 was also found to increase in the presence of IL-12, indicating that CD25 up-regulation is not the only mechanism by which IL-12 increases clonal expansion of the cells. IL-2 and IL-12 both act to increase expression of both CD25 and the IL-12R, thus providing positive cross-regulation of receptor expression. These results suggest that when cross-priming dendritic cells present class I/Ag and costimulatory ligands, and produce IL-12, naive CD8 T cells will begin to produce IL-2 and both receptors will be optimally up-regulated to insure that an effective response is generated.

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“…In pigs, the effect of IL-2 on CD25 expression is not known. Some binding motifs for transcription factors used for CD25 transcription in mice were found in the 5 -upstream region of the porcine CD25 gene (27). An in-depth study focusing on signaling pathway for porcine CD25 expression is essential to elucidate the mechanism used by virulent PRRSV to suppress CD25 expression by T cells.…”

Section: Effect Of Prrsv-infected Apcsmentioning

confidence: 99%

Effects of Porcine Reproductive and Respiratory Syndrome Virus-Infected Antigen-Presenting Cells on T Cell Activation and Antiviral Cytokine Production

2006

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The ability of porcine reproductive and respiratory syndrome virus (PRRSV) to suppress T cell expression of CD25 (alpha chain of interleukin [IL]-2 receptor), interferon-gamma (IFN-gamma), and tumor necrosis factor-alpha (TNF-alpha) was determined by flow cytometry in naive porcine T cells in response to mitogen (concanavalin A) and cytokine inducers (phorbol 12-myristate 13-acetate plus ionomycin [PMA/I]). Four PRRSV isolates of varying clinical virulence and three different types of porcine myeloid antigen-presenting cells (APCs) were used. T cells cultured with monocytes infected with virulent PRRSV (VR-2385, SDSU-73, and VR-2332), but not with a vaccine strain (Ingelvac PRRS MLV; Boehringer Ingelheim Vetmedica, St. Joseph, MO), demonstrated significantly reduced CD25 expression (%CD25(+)) and IFN-gamma expression (%IFN-gamma (+)) compared with T cells incubated with uninoculated monocyte cultures. T cells cultured with monocytes infected with all four PRRSV isolates demonstrated significantly reduced %TNF-alpha (+). The significant reduction of %CD25(+), %IFN-gamma (+), and %TNF-alpha (+) was not detected in T cells cultured with monocyte-derived macrophages (MDMs) and immature monocyte-derived dendritic cells (MDCs) infected with any PRRSV isolates. Heat-inactivated PRRSV did not induce significantly reduced T cell responses in any APC cultures. The reduction of T cell response in monocyte cultures was not due to PRRSV-induced T cell death. Gene expression of IL-10 detected by semiquantitative reverse transcriptase-polymerase chain reaction was significantly increased in virulent PRRSV-infected monocyte cultures after PMA/I, but not concanavalin A, stimulation compared with IL-10 gene expression from uninoculated monocyte cultures. Increased IL-10 gene expression contributed to significantly reduced %IFN-gamma (+) and %TNF-alpha (+), but not %CD25(+), as determined by IL-10 neutralization assay. This study reports that PRRSV has the ability to suppress T cell responses. The suppressive ability of PRRSV is associated with viral virulence and is mediated by virus-infected monocytes, but not by virus-infected MDMs and immature MDCs.

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Cloning and Chromosomal Assignment of the Porcine Interleukin-2 Receptor Alpha(IL-2R.ALPHA.) Gene.

Cited by 5 publications

References 33 publications

Alignment of VIM, MRC1, GAD2, and IL2RA genes on swine chromosome 10q by in situ hybridization and RH mapping

Alignment of VIM, MRC1, GAD2, and IL2RA genes on swine chromosome 10q by in situ hybridization and RH mapping

The Roles of IL-12 in Providing a Third Signal for Clonal Expansion of Naive CD8 T Cells

Effects of Porcine Reproductive and Respiratory Syndrome Virus-Infected Antigen-Presenting Cells on T Cell Activation and Antiviral Cytokine Production

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