Cloning and expression analysis of pepper chlorophyll catabolite reductase gene CaRCCR

Abstract

ABSTRACT. Opening the porphyrin macrocycle of pheophorbide a and forming the primary fluorescent chlorophyll catabolites are key steps in the chlorophyll catabolism pathway. These steps are catalyzed by pheophorbide a oxygenase and red chlorophyll catabolite reductase (RCCR). In this study, a novel RCCR gene, CaRCCR, was isolated from the pepper (Capsicum annuum L.). The full-length CaRCCR complementary DNA is comprised of 1173 bp, contains an open reading frame of 945 bp, and encodes a 314-amino acid protein. This deduced protein belongs to the ferredoxin-dependent bilin reductase family. Amino acid sequence alignment showed that CaRCCR shared a high homology to other higher plant RCCR proteins. CaRCCR expression, as determined by quantitative real-time polymerase chain reaction, was higher in the leaves than the roots, stems, flowers, and immature fruits. CaRCCR expression was almost constant during Cloning and expression analysis of CaRCCR in pepper all phases of leaf development. It was upregulated by abscisic acid, methyl jasmonate, and salicylic acid. Moreover, CaRCCR was induced by high salinity and drought stress treatments; it was also slightly regulated by Phytophthora capsici. Taken together, these results suggest that CaRCCR is involved in defense responses to various stresses.

Deciphering the Role of Stay-Green Trait to Mitigate Terminal Heat Stress in Bread Wheat

“…CaRCCR gene up-regulation under biotic and abiotic stresses had been previously reported by Xiao et al [77]. The up-regulation of the RCCR gene is associated with the defense responses in plants under stress conditions [77]. The present study demonstrated that the expression pattern of CaO and Cab at 7DAA is proportional to the increase/decrease in the chlorophyll content and photosynthetic capacity in functional stay-green, non-functional stay-green, and non-stay-green genotypes under terminal heat stress.…”

“…The increase in the relative abundance of the gene transcript was more pronounced in the non-functional stay-green type at 7DAA and in the functional stay-green genotype at 14DAA. CaRCCR gene up-regulation under biotic and abiotic stresses had been previously reported by Xiao et al [77]. The up-regulation of the RCCR gene is associated with the defense responses in plants under stress conditions [77].…”

“…A previous study by Mueller and coworkers showed great percentage similarity in the amino acid sequences of CaO in Hordeum vulgare, Arabidopsis thaliana, Brachypodium distachyon, and Oryza sativa (OsCaO 1, OsCaO2) [76]. Xiao and coworkers showed high sequence homology between the RCCR gene sequences from Solanum lycopersicum, Nicotiana tabacum, Vitis vinifera, Ricinus communis, Fragaria vesca subsp vesca, Brassica napus, and Arabidopsis thaliana [77]. High sequence homology suggests that the genes are highly conserved during evolution [78].…”

Partial Activation of SA- and JA-Defensive Pathways in Strawberry upon Colletotrichum acutatum Interaction

“…WRKY70 encodes a transcription factor and is an important regulator in the interplay of SA- and JA-related plant defense responses (Li et al, 2004 ). AOS encodes an allene oxide synthase, which is responsible for JA synthesis, and its expression is also regulated by this hormone (Turner et al, 2002 ). PDF1.2 and JAR1 are genes that are commonly used to monitor JA responses (Staswick and Tiryaki, 2004 ; Pieterse et al, 2009 ), whereas LOX2 , which is involved in JA biosynthesis, is activated by a positive feedback loop (Sasaki et al, 2001 ).…”