Cloning and Expression Analysis of After-Ripening Related Genes in Panax ginseng Seed

Abstract

Panax ginseng enjoys a wide range of medicinal applications and good economic value, the market demand is large, but Panax ginseng seed’s after-ripening characteristic seriously restricts development of Panax ginseng industry. In this experiment, starting from the main action hormone ABA and GA of Panax ginseng, primers of GA and ABA related to Panax ginseng seed after-ripening were screened and designed, and the possible relationship between transcription factor WRKY gene and Panax ginseng seed after-ripening was probed. Semiquantitative and quantitative real-time PCR of these genes were performed. ABA and GA-related metabolizing enzymes play an important role in the process of Panax ginseng seed after-ripening, and WRKY transcription factor regulates gene expression. In this study, we cloned the PgGA2ox gene, which is the key metabolic enzyme of GA, and analyzed the genes by the bioinformatics software, which laid a foundation for studying the molecular mechanism of Panax ginseng seed after-ripening.