Cloning and expression analysis of HY5 transcription factor gene of safflower in response to light signal

Xian, Bin; Chen, Chao; Wang, Jie; Jiang, Chen; Wu, Qinghua; Ren, Chaoxiang; Pei, Jin

doi:10.1002/bab.2373

Cited by 7 publications

(5 citation statements)

References 42 publications

(54 reference statements)

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Section: Discussionmentioning

confidence: 99%

“…The bZIP TF HY5 plays a critical role in controlling flavonoid and anthocyanin accumulation in response to light ( Shin et al., 2007 ; Zhang et al., 2011 ; Xian et al., 2023 ). Overexpression of CtHY5 initially promoted CtCHS1 expression and flavonoid content in Carthamus tinctorius L. protoplasts ( Xian et al., 2023 ). Previous studies have also emphasized that three TFs (HY5, HYH, and TTG2) may directly participate in the regulation of anthocyanin synthesis in two varieties, ‘Quanhong’ and ‘Xuanhong’ ( Chen et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Metabolome combined with transcriptome profiling reveals the dynamic changes in flavonoids in red and green leaves of Populus × euramericana ‘Zhonghuahongye’

Yang,

Chen,

Zhang

et al. 2023

Front. Plant Sci.

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Flavonoids are secondary metabolites that have economic value and are essential for health. Poplar is a model perennial woody tree that is often used to study the regulatory mechanisms of flavonoid synthesis. We used a poplar bud mutant, the red leaf poplar variety 2025 (Populus × euramericana ‘Zhonghuahongye’), and green leaves as study materials and selected three stages of leaf color changes for evaluation. Phenotypic and biochemical analyses showed that the total flavonoid, polyphenol, and anthocyanin contents of red leaves were higher than those of green leaves in the first stage, and the young and tender leaves of the red leaf variety had higher antioxidant activity. The analyses of widely targeted metabolites identified a total of 273 flavonoid metabolites (114 flavones, 41 flavonols, 34 flavonoids, 25 flavanones, 21 anthocyanins, 18 polyphenols, 15 isoflavones, and 5 proanthocyanidins). The greatest difference among the metabolites was found in the first stage. Most flavonoids accumulated in red leaves, and eight anthocyanin compounds contributed to red leaf coloration. A comprehensive metabolomic analysis based on RNA-seq showed that most genes in the flavonoid and anthocyanin biosynthetic pathways were differentially expressed in the two types of leaves. The flavonoid synthesis genes CHS (chalcone synthase gene), FLS (flavonol synthase gene), ANS (anthocyanidin synthase gene), and proanthocyanidin synthesis gene LAR (leucoanthocyanidin reductase gene) might play key roles in the differences in flavonoid metabolism. A correlation analysis of core metabolites and genes revealed several candidate regulators of flavonoid and anthocyanin biosynthesis, including five MYB (MYB domain), three bHLH (basic helix-loop-helix), and HY5 (elongated hypocotyl 5) transcription factors. This study provides a reference for the identification and utilization of flavonoid bioactive components in red-leaf poplar and improves the understanding of the differences in metabolism and gene expression between red and green leaves at different developmental stages.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Metabolome combined with transcriptome profiling reveals the dynamic changes in flavonoids in red and green leaves of Populus × euramericana ‘Zhonghuahongye’

Yang,

Chen,

Zhang

et al. 2023

Front. Plant Sci.

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“…As a member of the Compositae family, sa ower's features such as achenes, leathery leaves and tiny tubular owers are not conducive to directly application for transient transformation. Protoplast is one of an approach, and the protoplast transient expression system of sa ower has been used for gene function analysis [19]. However, the preparation of sa ower protoplasts is affected by the owering period, and requires high experimental operations.…”

Section: Discussionmentioning

confidence: 99%

“…The extracts were nally dissolved in methanol, and analyzed by LC-MS system (Synapt, Waters, USA). The experimental methods referred to reference [19]. Sa ower callus transformed with empty vector was used as control.…”

Section: Agrobacterium-mediated Transient Transformationmentioning

confidence: 99%

The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L. (safflower)

Xian

Ren

et al. 2022

Preprint

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Background: Safflower (Carthamus tinctorius L.) is an important economic crop and traditional medicine riching in flavonoids, which can significantly improve cardiovascular and cerebrovascular diseases. Thus, many candidate genes for safflower flavonoid biosynthesis were cloned. However, due to the lack of homologous gene expression system, gene function research can only be carried out in model plants. It is necessary to establish gene function identification protocol in safflower. Results: In this study, using safflower callus as experimental material, Agrobacterium transient expression system and bilolistic transient expression system were established. In the Agrobacterium transient expression system, the highest transformation rate were obtained at original Agrobacterium density of OD600 0.4, concentration of OD600 0.6, infection for 20 min, co-culture for 3 d, and AS concentration at 100 μmol·L-1. In biolistic transient expression system, the highest transformation efficiency were observed at 1350 psi helium pressure, -0.8 bar vacuum degree, 6.5 cm flight distance, the bombardment once, 3 μg·shot-1 of plasmid amount, and 100 μg·shot-1 of gold particles amount. These two transient expression systems were used for the gene function analysis of CtCHS1 as an example. After overexpression, the relative expression of CtCHS1 was increased, especially in the Agrobacterium-transformed callus. The content of some flavonoids also changed, naringenin and genistein were significantly increased in Agrobacterium-transformed callus, and luteolin, luteolin-7-O-rutinoside and apigenin derivative were significantly decreased in biolistic-transformed callus. Conclusion: Using safflower callus as the experimental material, Agrobacterium transient expression system and bilolistic transient expression system with high expression efficiency were successfully established, and demonstrating that both transient expression systems can be used to verify gene function. The safflower callus transient expression system will be a useful methods for further analysis in gene function analysis of flavonoid biosynthesis in safflower.

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“…The bZIP TF HY5 plays a critical role in controlling flavonoid and anthocyanin accumulation in response to light (Shin et al, 2007;Zhang et al, 2011;Xian et al, 2023). Overexpression of CtHY5 initially promoted CtCHS1 expression and flavonoid content in Carthamus tinctorius L. protoplasts (Xian et al, 2023). Previous studies have also emphasized that three TFs (HY5, HYH, and TTG2) may directly participate in the regulation of anthocyanin synthesis in two varieties, 'Quanhong' and 'Xuanhong' .…”

Section: Construction Of the Gene Coexpression Network During Leaf Co...mentioning

confidence: 99%

Bioactive Compounds, Functional Ingredients, Antioxidants, and Health Benefits of Edible Plants

2024

Frontiers Research Topics

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Frontiers is more than just an open access publisher of scholarly articles: it is a pioneering approach to the world of academia, radically improving the way scholarly research is managed. The grand vision of Frontiers is a world where all people have an equal opportunity to seek, share and generate knowledge. Frontiers provides immediate and permanent online open access to all its publications, but this alone is not enough to realize our grand goals. Frontiers journal seriesThe Frontiers journal series is a multi-tier and interdisciplinary set of openaccess, online journals, promising a paradigm shift from the current review, selection and dissemination processes in academic publishing. All Frontiers journals are driven by researchers for researchers; therefore, they constitute a service to the scholarly community. At the same time, the Frontiers journal series operates on a revolutionary invention, the tiered publishing system, initially addressing specific communities of scholars, and gradually climbing up to broader public understanding, thus serving the interests of the lay society, too. Dedication to qualityEach Frontiers article is a landmark of the highest quality, thanks to genuinely collaborative interactions between authors and review editors, who include some of the world's best academicians. Research must be certified by peers before entering a stream of knowledge that may eventually reach the public -and shape society; therefore, Frontiers only applies the most rigorous and unbiased reviews. Frontiers revolutionizes research publishing by freely delivering the most outstanding research, evaluated with no bias from both the academic and social point of view. By applying the most advanced information technologies, Frontiers is catapulting scholarly publishing into a new generation. What are Frontiers Research Topics?Frontiers Research Topics are very popular trademarks of the Frontiers journals series: they are collections of at least ten articles, all centered on a particular subject. With their unique mix of varied contributions from Original Research to Review Articles, Frontiers Research Topics unify the most influential researchers, the latest key findings and historical advances in a hot research area.

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Cloning and expression analysis of HY5 transcription factor gene of safflower in response to light signal

Cited by 7 publications

References 42 publications

Metabolome combined with transcriptome profiling reveals the dynamic changes in flavonoids in red and green leaves of Populus × euramericana ‘Zhonghuahongye’

Metabolome combined with transcriptome profiling reveals the dynamic changes in flavonoids in red and green leaves of Populus × euramericana ‘Zhonghuahongye’

The establishment of transient expression systems and their application for gene function analysis of flavonoid biosynthesis in Carthamus tinctorius L. (safflower)

Bioactive Compounds, Functional Ingredients, Antioxidants, and Health Benefits of Edible Plants

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