Cloning and Expression Analysis of <i>RrMYB</i>113 Gene Related to Anthocyanin Biosynthesis in <i>Rosa rugose</i>

Abstract

Anthocyanin is one of water-soluble natural pigments widely existing in flowers, fruits, stems, leaves and seeds of plants, and it is the major factor conferring pink or red to the petals of Rosa rugosa. MYB TFs play an important role in the anthocyanin synthesis in plants. This work aimed to clone the MYB gene related to anthocyanin synthesis in the petals of Rosa rugosa, and explore the relationship between them to lay a good foundation for gene engineering improvement of R. rugosa. Based on the transcriptional data, a full-length cDNA sequence of MYB Gene, RrMYB113 (GenBank accession Nos MG720012), was cloned at the first time from the petals of Rosa rugosa "Zi zhi" with RT-PCR and RACE methods. The full-length cDNA is 885 bp with an open reading frame of 654 bp, encoding 216 amino acids. The derived RrMYB113 protein has a molecular weight of 25,297.64 Da, a calculated pI of 9.61, a R 2 R 3 -MYB domain and bHLH binding domain, and it also has the signature motifs ((A/S/G)NDV and KPRPR(T/S)), thus belonging to Sg6 R 2 R 3 -MYB subfamily. In the secondary structure of RrMYB113 protein, there is 37.04% α-helix, 39.81% random coil, 14.81% extended peptide chain, and 8.33% β-corner. There is no transmembrane domain and no signal peptide cleavage site, seventeen Ser phosphorylation sites, fifteen Thr phosphorylation sites, four Tyr phosphorylation sites, and no O-glycosylation sites. The expression of RrMYB113 increased with the color deepening in petals, and it expressed at a higher level in petals than in other tissues of R. rugosa "Zi zhi". These results are meaningful to reveal that RrMYB113 might be an important regulator in anthocyanin biosynthesis and coloration in the petals of R. rugosa.

Ectopic Expression of Multiple Chrysanthemum (Chrysanthemum × morifolium) R2R3-MYB Transcription Factor Genes Regulates Anthocyanin Accumulation in Tobacco

“…Within the variable C-terminal region, the conserved SG6 motif has been widely identified as defining the anthocyanin-related proteins [47,48,49,50,51]. At an early stage, the SG6 motif was identified as KPRPR[S/T]F in Arabidopsis [47] and thereafter modified to [K/R]P[Q/R]P[Q/R] based on a study in Asiatic lily [48].…”

The MYB transcription factor RcMYB1 plays a central role in rose anthocyanin biosynthesis

“…Only a few studies have reported that MYB-TFs may be involved in the anthocyanin biosynthesis of rose. RrMYB113 and RhMYB10 were supposed to play a role in anthocyanin synthesis [ 31 , 32 ]. RrMYB5 and RrMYB10 are induced by wounding, and their expression leads to increased accumulation of anthocyanins and proanthocyanidins [ 33 ].…”

Advances in the application of biosynthesis and metabolic engineering of flavonoids in plants

“…Most of the structure genes such as CHS, CHI, F3H, F3'H, DFR, and ANS (encoding chalcone synthase, chalcone isomerase, flavanone 3-hydroxylase; flavonoid 3'-hydroxylase, dihydroflavonol 4-reductase, and anthocyanidin synthase) have been cloned in various plants (Petroni and Tonelli 2011, Goswami et al 2018, Zhang et al 2018b). In addition, a few transcription factors have been characterized, including members from the v-myb avian myeloblastosis viral oncogene homolog (MYB), basic Helix-Loop-Helix (bHLH) and WD40 domain-containing protein (WD40) families (Wang et al 2018, Zou et al 2018. Structural genes in the pathway are largely regulated at the level of transcription.…”