Cloning and Expression Analysis of 1 L-myo-Inositol-1-phosphate Synthase Gene from Ricinus communis L.

Wei, Wei; Dai, Xiao; Wang, Yong; Chen, Yu; Gou, Chunbao; Chen, Fang

doi:10.1515/znc-2010-7-814

Cited by 8 publications

(6 citation statements)

References 24 publications

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“…In this study, in leaves of drought-resistant strain FS43 and sensitive strain FS38, MIPS were up-regulated following water deficit (Tab.1). This result is in concordance with a previous study, which indicated that MIPS plays an important role in drought-resistance (Wei, W. et al, 2010). …”

supporting

confidence: 94%

Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit

Ding¹,

Guo²,

Ruan³

et al. 2015

Proceedings of the 2015 International Forum on Energy, Environment Science and Materials

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supporting

confidence: 94%

Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit

Ding¹,

Guo²,

Ruan³

et al. 2015

Proceedings of the 2015 International Forum on Energy, Environment Science and Materials

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“…India, Brazil and China are the major oil crop producers in the world and, recently, biodiesel production has received wide attention in Brazil, where the Program for Production and Use of Biodiesel (PNPB) has identified castor bean as the ideal species for oil production which, in addition, could help to promote social development in the semi-arid regions of Brazil (Cesar and Batalha, 2010). Its agronomic importance stimulated molecular research in R. communis related to seed maturation (Cagliari et al , 2010; Sánchez-García et al , 2010; Loss-Morais et al , 2013), plant development (O'Leary et al , 2011), and responses to biotic and abiotic stresses (Wei et al , 2010; Maciel et al , 2011). Characterization and expression profiles of several genes from R. communis have been reported (Cagliari et al , 2010; Chileh et al , 2010; Wei et al , 2010; Maciel et al , 2011).…”

Section: Introductionmentioning

confidence: 99%

“…Its agronomic importance stimulated molecular research in R. communis related to seed maturation (Cagliari et al , 2010; Sánchez-García et al , 2010; Loss-Morais et al , 2013), plant development (O'Leary et al , 2011), and responses to biotic and abiotic stresses (Wei et al , 2010; Maciel et al , 2011). Characterization and expression profiles of several genes from R. communis have been reported (Cagliari et al , 2010; Chileh et al , 2010; Wei et al , 2010; Maciel et al , 2011). In this study we focus on seed germination and seedling growth, since a better understanding of the biochemical and molecular aspects of these processes in R. communis is crucial for the breeding of high-yielding varieties adapted to various growing environments (Severino and Auld, 2013).…”

Section: Introductionmentioning

confidence: 99%

Identification of reference genes for gene expression studies during seed germination and seedling establishment in Ricinus communis L.

et al. 2014

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Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is an important technology to analyse gene expression levels during plant development or in response to different treatments. An important requirement to measure gene expression levels accurately is a properly validated set of reference genes. In this context, we analysed the potential use of 17 candidate reference genes across a diverse set of samples, including several tissues, different stages and environmental conditions, encompassing seed germination and seedling growth in Ricinus communis L. These genes were tested by RT-qPCR and ranked according to the stability of their expression using two different approaches: GeNorm and NormFinder. GeNorm and Normfinder indicated that ACT, POB and PP2AA1 comprise the optimal combination for normalization of gene expression data in inter-tissue (heterogeneous sample panel) studies. We also describe the optimal combination of reference genes for a subset of root, endosperm and cotyledon samples. In general, the most stable genes suggested by GeNorm are very consistent with those indicated by NormFinder, which highlights the strength of the selection of reference genes in our study. We also validated the selected reference genes by normalizing the expression levels of three target genes involved in energy metabolism with the reference genes suggested by GeNorm and NormFinder. The approach used in this study to identify stably expressed genes, and thus potential reference genes, was applied successfully for R. communis and it provides important guidelines for RT-qPCR studies in seeds and seedlings for other species (especially in those cases where extensive microarray data are not available).

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“…WLR measurements using detached leaves were already applied to characterize drought tolerance in crops like wheat, maize, wild barley and sunflower (Rampino et al 2006;Clarke and McCaig 1982;Ristic and Jenks 2002;Suprunova et al 2004;Teulat et al 2003). RWC was a relevant screening tool for drought tolerance in cereals (Teulat et al 2003;Muthurajan et al 2010) and castor bean (Ricinus communis L.) (Wei et al 2010). Characterization of our genotypes by leaf WLR and RWC after 4h confirms that F. chiloensis is drought tolerant.…”

Section: Discussionmentioning

confidence: 63%

A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers

Razavi

Keyser²,

Riek³

et al. 2011

Euphytica

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Drought stress is one of the most important environmental factors that limit plant growth and development, thus reducing yield. The objective of the present research was to correlate the genetic structure of different Fragaria genotypes, as assessed by Expressed Sequence Tag (EST) and Amplified Fragment Length Polymorphism (AFLP) markers, and plant responses to drought stress. Firstly, physiological parameters related to the plant response to drought stress such as leaf relative water content (RWC) and water losing rate (WLR) were measured. WLR and RWC were compared for 20 cultivars of the octaploid Fragaria 9 ananassa, two ecotypes of the diploid species F. vesca and one octaploid species F. chiloensis. These parameters could discriminate genotypes showing a contrasting response to water stress. Secondly, AFLP and ESTs were compared in terms of their information content and efficiency in the study of genetic diversity and relationships among these 23 Fragaria genotypes. To evaluate the genetic basis for the observed variation in the measured physiological parameter, the effect of specific AFLP/EST loci on WLR and RWC for the different Fragaria genotypes was quantified by Kruskal-Wallis analysis. By Mantel testing, the hierarchical clustering of the Fragaria genotypes based on associated EST or AFLP markers was compared to the observed eco-physiological relevant grouping. A better discriminating capacity for associated markers was noted, enabling a functional marker selection approach to screen the strawberry gene pool for drought tolerance. Correlation of EST markers to leaf RWC and WLR enforces them as potential candidate genes in control of plant responses to drought stress in Fragaria sp.

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Cloning and Expression Analysis of 1 L-myo-Inositol-1-phosphate Synthase Gene from Ricinus communis L.

Cited by 8 publications

References 24 publications

Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit

Protein Profile Changes of Two Chinese Fir Genotypes under Short-term Water Deficit

Identification of reference genes for gene expression studies during seed germination and seedling establishment in Ricinus communis L.

A method for testing drought tolerance in Fragaria based on fast screening for water deficit response and use of associated AFLP and EST candidate gene markers

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