Cloning and expression analysis of betalain biosynthesis genes in Amaranthus tricolor

Zheng, Xiao; Liu, Shengcai; Cheng, Chunzhen; Guo, R. S.; Chen, Yukun; Xie, Liji; Mao, Yong; Lin, Yuling; Zhang, Zi-Hao; Zhang, Lai

doi:10.1007/s10529-015-2021-z

Cited by 33 publications

(27 citation statements)

References 14 publications

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“…The production of betacyanins by seedlings obtained from callus cell line of A. tricolor was also reported and positively influenced by light and cytokinins [ 131 ]. The expression of five betalains’ biosynthesis genes was modulated by phytohormones but there are presumably also other factors that influence betalain production [ 132 ].…”

Section: Amaranthaceaementioning

confidence: 99%

Betalains in Some Species of the Amaranthaceae Family: A Review

Miguel

2018

Antioxidants

132

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Natural pigments are largely distributed in the plant kingdom. They belong to diverse groups, with distinct biochemical pathways. Betalains with colours that range from yellow to red-violet can de divided into two main subgroups: betaxanthins and betacyanins. These types of pigments are confined into 13 families of the order Caryophyllales and in some genera of higher fungi (Amanita muscaria, Hygrocybe and Hygrophorus). The Amaranthaceae family includes diverse genera in which betalains are present: Alternanthera, Amaranthus, Beta, Chenopodium, Celosia and Gomphrena. The biosynthesis of betalains and their general biological properties were reviwed in the present work. In addition, the types of betalains present in some species of the aforementioned genera, their stability and production, as well as biological attributes, were reviewed.

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Section: Amaranthaceaementioning

confidence: 99%

Betalains in Some Species of the Amaranthaceae Family: A Review

Miguel

2018

Antioxidants

132

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“…The observed expression trends for miR166 and HD-ZIP were consistent with the results of a previous rice study (Liu et al 2009). Meanwhile, gibberellins can strongly inhibit miR166 expression and the accumulation of anthocyanins (Ahmad & Iqbal 2012) or betalains (Zheng et al 2016). Therefore, we speculate that miR166 may positively regulate betalain biosynthesis, while having the opposite effect on anthocyanin biosynthesis.…”

Section: Members Of the Mirl66 Family May Be Important Positive Regulsupporting

confidence: 89%

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Supplemental Information 6: Supplementary Table S1 Identification of Known and Some Novel Mature miRNA Sequences and Analysis I

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Betalains are abundant in amaranth plants. Additionally, the betalain molecular structure and metabolic pathway differ from those of betanin in beet plants. To date, only a few studies have examined the regulatory roles of miRNAs in betalain biosynthesis in plants. Thus, we constructed small RNA libraries for the red and green sectors of amaranth leaves to identify miRNAs associated with betalain biosynthesis. We identified 198 known and 41 novel miRNAs. Moreover, 216 miRNAs were distributed in 44 miRNA families, including miR156, miR159, miR160, miR166, miR172, miR319, miR167, miR396, and miR398. An analysis of all unigene sequences in an amaranth transcriptome database resulted in the detection of 493 target genes for the 239 screened miRNAs. The targets included SPL2, ARF18, ARF6, and NAC. A quantitative real-time polymerase chain reaction validation of 20 miRNAs and nine target genes revealed expression-level differences between the red and green sectors of amaranth leaves. This study involved the application of an Illumina sequencing platform to identify miRNAs regulating betalain metabolism in amaranth plants. The data presented herein may provide insights into the molecular mechanisms underlying the regulation of betalain biosynthesis in amaranth and other plant species.

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“…OsUGT1 is clustered to 7- O -G group, suggesting that OsUGT1 may attack the hydroxyl group at C-7 position of flavonoids. On the other hand, OsUGT1 is clustered as the same phylogenetic group with a putative 5,6-dihydroxyindoline-2-carboxylic acid ( cyclo -DOPA) 5- O -glucosyltransferase (KP174811.1), suggesting OsUGT1 may act on the flavonoids showing similar structure with cyclo -DOPA [ 26 ]. Cyclo -DOPA has a bicyclic structure consisting of a six-membered benzene ring fused to a five-membered nitrogen-containing ring ( Figure 3 B).…”

Section: Resultsmentioning

confidence: 99%

Transcriptome-Wide Identification of an Aurone Glycosyltransferase with Glycosidase Activity from Ornithogalum saundersiae

Yuan

Liu

Yang

et al. 2018

Genes

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Aurone glycosides display a variety of biological activities. However, reports about glycosyltransferases (GTs) responsible for aurones glycosylation are limited. Here, the transcriptome-wide discovery and identification of an aurone glycosyltransferase with glycosidase activity is reported. Specifically, a complementary DNA (cDNA), designated as OsUGT1, was isolated from the plant Ornithogalum saundersiae based on transcriptome mining. Conserved domain (CD)-search speculated OsUGT1 as a flavonoid GT. Phylogenetically, OsUGT1 is clustered as the same phylogenetic group with a putative 5,6-dihydroxyindoline-2-carboxylic acid (cyclo-DOPA) 5-O-glucosyltransferase, suggesting OsUGT1 may be an aurone glycosyltransferase. The purified OsUGT1 was therefore used as a biocatalyst to incubate with the representative aurone sulfuretin. In vitro enzymatic analyses showed that OsUGT1 was able to catalyze sulfuretin to form corresponding monoglycosides, suggesting OsUGT1 was indeed an aurone glycosyltransferase. OsUGT1 was observed to be a flavonoid GT, specific for flavonoid substrates. Moreover, OsUGT1 was demonstrated to display transglucosylation activity, transferring glucosyl group to sulfuretin via o-Nitrophenyl-β-d-glucopyranoside (oNP-β-Glc)-dependent fashion. In addition, OsUGT1-catalyzed hydrolysis was observed. This multifunctionality of OcUGT1 will broaden the application of OcUGT1 in glycosylation of aurones and other flavonoids.

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Cloning and expression analysis of betalain biosynthesis genes in Amaranthus tricolor

Abstract: The changes in betacyanin levels among various tissues and following phytohormone treatments were related to the differences in betalain biosynthesis gene expression levels.

Cited by 33 publications

References 14 publications

Betalains in Some Species of the Amaranthaceae Family: A Review

Betalains in Some Species of the Amaranthaceae Family: A Review

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Transcriptome-Wide Identification of an Aurone Glycosyltransferase with Glycosidase Activity from Ornithogalum saundersiae

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