Cloning and expression analysis of an anthocyanidin synthase gene homologue from Brassica carinata

Yan, Mingli; Ding, Suping; Liu, Li‐Li; Yin, Xiao‐Ming; Shu, Jiabin

doi:10.1007/s12041-014-0387-7

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…EBGs ( chalcone synthase , CHS ; chalcone isomerase , CHI ; flavanone 3-hydroxylase , F3H ; flavonoid 3’-hydroxylase , F3’H ) are the common flavonoid pathway genes which are involved in the biosynthesis of all downstream flavonoids. LBGs ( dihydroflavonol 4-reductase , DFR ; anthocyanidin synthase/leucoanthocyanin dioxygenase , ANS / LDOX ; uridine diphosphate-glucose: flavonoid 3-O-glucosyltransferase , UFGT ) are genes regulating the biosynthesis of anthocyanins ( Petroni and Tonelli, 2011 ; Yan et al, 2014 ; Liu et al, 2018 ). The EBGs are regulated by a class of R2R3–MYB transcription factors, while the regulation of LBGs requires a ternary complex of MYB–bHLH–WD40 (MBWs), which binds the promoters of target genes and then activate their transcription, leading to a higher accumulation of the anthocyanin level ( Lepiniec et al, 2006 ; Xu et al, 2014 ; Kim et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Zhang

et al. 2021

Front. Genet.

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Anthocyanins contribute to most colors of plants and play protective roles in response to abiotic stresses. Brassica napus is widely cultivated worldwide as both an oilseed and a vegetable. However, only several high anthocyanin-containing cultivars have been reported, and the mechanisms of anthocyanin accumulation have not been well-elucidated in B. napus. Here, the phenotype, comparative whole-genome identification, and gene expression analysis were performed to investigate the dynamic change of the anthocyanin content and the gene expression patterns of anthocyanin biosynthetic genes (ABGs) in B. napus. A total of 152 ABGs were identified in the B. napus reference genome. To screen out the critical genes involved in anthocyanin biosynthesis and accumulation, the RNA-seq of young leaves of two B. napus lines with purple leaves (PL) or green leaves (GL), and their F1 progeny at 41, 91, and 101 days were performed to identify the differentially expressed genes. The comparative expression analysis of these ABGs indicated that the upregulation of TT8 together with its target genes (such as DFR, ANS, UFGT, and TT19) might promote the anthocyanin accumulation in PL at the early developmental stage (41–91 days). While the downregulation of those ABGs and anthocyanin degradation at the late developmental stage (91–101 days) might result in the decrease in anthocyanin accumulation. Our results would enhance the understanding of the regulatory network of anthocyanin dynamic accumulation in B. napus.

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Section: Introductionmentioning

confidence: 99%

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Zhang

et al. 2021

Front. Genet.

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“…ANS is encoded by a mini-gene family in many plants. ANS gene have been cloned and functionally analyzed in Theobroma cacao (Liu et al, 2013), Brassica carinata (Yan et al, 2014), Brassica juncea (Yan et al, 2011), Fructus mori (Qi et al, 2014), Punica granatum L. (Ben-Simhon et al, 2015), Malus spectabilis (Zhang et al, 2015), and Pyrus communis L. ; however, few reports on regulation of anthocyanin accumulation by ANS gene in peach fruit skin are available. In the present study, an ANS gene (PpANS) was isolated from peach fruit skin by using RT-PCR technique to determine the function of PpANS in peach fruit.…”

Section: Cloning Of Full-length Cdna Of Ppansmentioning

confidence: 99%

Molecular Cloning and Characterization of an Anthocyanidin Synthase Gene in Prunus persica (L.) Batsch

Wang

et al. 2017

Not Bot Horti Agrobo

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To elucidate the effect of anthocyanidin synthase (ANS) gene on anthocyanin accumulation in fruit skin of Prunus persica (L.) Batsch cv. 'Chunmei', this study cloned and characterized an ANS gene (PpANS) from peach. PpANS (GenBank accession No. KX760117) was encoded by a 1074 bp-long open reading frame (ORF) corresponding to a polypeptide consisting of 358 amino acids with a molecular mass of 40.45 kD and an isoelectric point of 5.46. PpANS contains a conserved 2-oxoglutarate-and iron-dependent dioxygenases and non-haem dioxygenase binding regions. PpANS shared high similarities to angiosperm ANS and displayed the closest genetic relationship to Prunus domestica. Real-time PCR analysis indicated that PpANS was highly expressed in fruit skin, flesh and flowers, and peach fruit skin showed the highest transcript level of PpANS. Anthocyanin accumulation analysis indicated that it was highly accumulated in fruit skin and flesh of peach. Changes in the transcript level were highly correlated with anthocyanin content in the different tissues of peach. Prokaryotic expression analysis showed PpANS that protein can be expressed correctly in E. coli, and the size of PpANS recombinant protein was consistent with its predicted size. In vitro enzyme activity assay revealed that recombinant PpANS protein could catalyze the formation the cyanidin from leucocyanidin.These results indicated that PpANS was responsible for anthocyanin accumulation in P. persica.

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“…The anthocyanin content of yellow raspberry decreased significantly [ 23 ]. The BjANS gene of Brassica juncea was expressed in both black seed coat and embryo, while in yellow seed coat, the deletion of BjANS gene expression prevented the biosynthesis of proanthocyanidins, so the seeds were yellow due to the transparency of the seed coat [ 24 ]. Eun-Young Kim et al [ 25 ] identified two new allelic variants of ANS genes, which could cause the deletion of onion anthocyanin synthesis.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Expression Analysis of ANS Family in Strawberry Fruits at Different Coloring Stages

Feng

Yang

et al. 2023

IJMS

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To elucidate the structural characteristics, phylogeny and biological function of anthocyanin synthase (ANS) and its role in anthocyanin synthesis, members of the strawberry ANS gene family were obtained by whole genome retrieval, and their bioinformatic analysis and expression analysis at different developmental stages of fruit were performed. The results showed that the strawberry ANS family consisted of 141 members distributed on 7 chromosomes and could be divided into 4 subfamilies. Secondary structure prediction showed that the members of this family were mainly composed of random curls and α-helices, and were mainly located in chloroplasts, cytoplasm, nuclei and cytoskeletons. The promoter region of the FvANS gene family contains light-responsive elements, abiotic stress responsive elements and hormone responsive elements, etc. Intraspecific collinearity analysis revealed 10 pairs of FvANS genes, and interspecific collinearity analysis revealed more relationships between strawberries and apples, grapes and Arabidopsis, but fewer between strawberries and rice. Chip data analysis showed that FvANS15, FvANS41, FvANS47, FvANS48, FvANS49, FvANS67, FvANS114 and FvANS132 were higher in seed coat tissues and endosperm. FvANS16, FvANS85, FvANS90 and FvANS102 were higher in internal and fleshy tissues. Quantitative real-time PCR (qRT-PCR) showed that the ANS gene was expressed throughout the fruit coloring process. The expression levels of most genes were highest in the 50% coloring stage (S3), such as FvANS16, FvANS19, FvANS31, FvANS43, FvANS73, FvANS78 and FvANS91. The expression levels of FvANS52 were the highest in the green fruit stage (S1), and FvANS39 and FvANS109 were the highest in the 20% coloring stage (S2). These results indicate that different members of the FvANS gene family play a role in different pigmentation stages, with most genes playing a role in the expression level of the rapid accumulation of fruit coloring. This study lays a foundation for further study on the function of ANS gene family.

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Cloning and expression analysis of an anthocyanidin synthase gene homologue from Brassica carinata

Abstract: 2014 Cloning and expression analysis of an anthocyanidin synthase gene homologue from Brassica carinata. J. Genet. 93, 513-516]

Cited by 8 publications

References 8 publications

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Whole-Genome Identification and Comparative Expression Analysis of Anthocyanin Biosynthetic Genes in Brassica napus

Molecular Cloning and Characterization of an Anthocyanidin Synthase Gene in Prunus persica (L.) Batsch

Genome-Wide Identification and Expression Analysis of ANS Family in Strawberry Fruits at Different Coloring Stages

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