Cloning and expression analysis of the follicle-stimulating hormone receptor (FSHR) gene in the reproductive axis of female yaks (Bos grunniens)

Xia, Yi; Wang, Q.; He, Xiang-Dong; Chen, Y.; Jige, Mo-Ti; Zi, Xiang-Dong

doi:10.1016/j.domaniend.2019.07.011

Cited by 12 publications

(9 citation statements)

References 48 publications

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“…Relevant FSHR studies have indicated that mutation of the 5′UTR region in this gene can improve the litter size of both small-tailed Han sheep and Hu sheep [ 34 , 35 ]. The FSHR gene is a member of the G protein-coupled receptor family and is only expressed in mammalian follicular granulosa cells [ 36 ]. Its expression is increased with the growth of healthy follicles [ 37 ], playing a core role in promoting follicular maturation and increasing ovulation.…”

Section: Discussionmentioning

confidence: 99%

CYP19A1 May Influence Lambing Traits in Goats by Regulating the Biological Function of Granulosa Cells

Zhang

Chen

Zhou

et al. 2022

Animals

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Abnormal expression of CYP19A1, a gene related to steroid hormone synthesis, causes steroid hormone disruption and leads to abnormal ovulation in granulosa cells. However, the exact mechanism of CYP19A1 regulation is unclear. In this study, we confirmed the localization of CYP19A1 in goat ovarian tissues using immunohistochemistry. Subsequently, we investigated the effects of CYP19A1 on granulosa cell proliferation, steroid hormone secretion, and expression of candidate genes for multiparous traits by overexpressing and silencing CYP19A1 in goat granulosa cells (GCs). The immunohistochemistry results showed that CYP19A1 was expressed in all types of follicular, luteal, and granulosa cells, with subcellular localization results revealing that CYP19A1 protein was mainly localized in the cytoplasm and nucleus. Overexpression of CYP19A1 significantly increased the mRNA levels of CYP19A1, FSHR, and INHBA, which are candidate genes for multiple birth traits in goats. It also promoted cell proliferation, PCNA and Cyclin E mRNA levels in granulosa cells, and secretion of estrogen and progesterone. However, it inhibited the mRNA levels of STAR, CYP11A1, and 3βSHD, which are genes related to steroid synthesis. Silencing CYP19A1 expression significantly reduced CYP19A1, FSHR, and INHBA mRNA levels in granulosa cells and inhibited granulosa cell proliferation and PCNA and Cyclin E mRNA levels. It also reduced estrogen and progesterone secretion but enhanced the mRNA levels of STAR, CYP11A1, and 3βSHD. CYP19A1 potentially influenced the lambing traits in goats by affecting granulosa cell proliferation, hormone secretion, and expression of candidate genes associated with traits for multiple births.

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Section: Discussionmentioning

confidence: 99%

CYP19A1 May Influence Lambing Traits in Goats by Regulating the Biological Function of Granulosa Cells

Zhang

Chen

Zhou

et al. 2022

Animals

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“…Gene FSHR was associated with promoting follicle of Yaks (Xia et al, 2020). Wang et al (2014) suggested that polymorphism of gene CD46 is associated with the occurrence of mastitis in dairy cow.…”

Section: Resultsmentioning

confidence: 97%

Detection of Selection Signals in Cattle Populations by Pca

Olšanská

Kasarda

Lehocká

et al. 2020

AGR

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The presented study provides a genome-wide scan of selection signals in cattle by principal component analysis (PCA). The aim was to identify SNP affected by intensive selection based on package PCAdapt implemented under software R. This analysis provided insight into the association between the SNP frequencies related to population differentiation. The four cattle populations were involved in the analysis (Slovak Spotted cattle, Ayrshire, Swiss Simmental and Holstein) with overall 272 of genotyped individuals. After applying quality control, the final dataset consisted of 35 675 SNPs, with an overall length of 2496.14 Mb and average space between adjacent SNP 70.03 ± 76.1 kb. After performing PCA analysis, the uniqueness of the breeds was revealed. On the other hand, a close genetic relationship and eleven SNPs affected by selection were found, with a position close to 162 genes involved in the various biological processes. The majority of genes were involved in the positive regulation of adenylate cyclase activity, embryo development and somatic diversification of immune receptors via somatic mutation. Several candidate genes for genetic control of the immune system (DNAJB9), muscle development (SEPT7, TRIM32, ROCK1, NRAP, PZDZ8, HSPA12A and FGFR2), milk production (SOCS5, CD46), reproduction (LHCGR, EEPD1, FSHR) and coat colour (KIT) were identified. Our results provide insights into the regions of the genome affected by the intensive selection of analysed cattle populations.

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“…The oviduct is an important site for fertilization in yaks and previous studies focused on its morphology ( 18 ), POxia-inducible factor-1 alpha ( 19 ), ERK1/2 ( 20 ), estrogen receptor genes ( 21 ), follicle-stimulating hormone receptor (FSHR) gene ( 22 ), DNA damage induced transcript 3 ( 23 ), and apoptosis-related genes in the yak oviduct ( 24 ). There are currently no transcriptomic studies on the different parts of the yak oviduct, and there is a lack of research on the fertilization mechanism from the perspective of mRNA.…”

Section: Discussionmentioning

confidence: 99%

Identification of key genes and biological pathways in different parts of yak oviduct based on transcriptome analysis

Zhang

Zhao

et al. 2022

Front. Vet. Sci.

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The oviduct consists of three parts: the infundibulum (In), ampulla (Am), and isthmus (Is). These have the same histological structure, but different physiological functions. In this study, transcriptomics was used to analyze mRNA in these three parts of yak oviduct. The results showed that there were 325 up-regulated genes and 282 down-regulated genes in the infundibulum and ampulla. Moreover, there were 234 up-regulated genes and 776 down-regulated genes in the isthmus and ampulla, as well as 873 up-regulated genes and 297 down-regulated genes in the infundibulum and isthmus. The expression of C3 in the infundibulum was significantly higher than that in the ampulla and isthmus. The expression of FAU in the isthmus was significantly lower than that in the ampulla and infundibulum, and the expression of EEF1A1 in the ampulla was significantly higher than that in the ampulla and infundibulum. When the infundibulum was compared with the ampulla and isthmus, it was found that the up-regulated genes were enriched in the lysosome, phagosome, staphylococcus aureus infection, and leishmaniasis pathway. When the isthmus was compared with the ampulla and infundibulum, the up-regulated genes were present in the apoptosis pathway, oxidative phosphorylation, and viral myocarditis pathway. When the isthmus was compared with the infundibulum and ampulla, the down-regulated pathways were protein processing in the endoplasmic reticulum and the endocytosis. The Epstein–Barr virus infection pathway was up-regulated according to a comparison of the isthmus and infundibulum and was down-regulated based on a comparison of the isthmus and ampulla. Transcriptional misregulation in the Middle East pathway was up-regulated based on a comparison of the isthmus and ampulla and was down-regulated based on a comparison of the isthmus and infundibulum. ERBB2, JUP, CTNND1, and KRT7 were defined as the hub genes of the yak oviduct. The results of this study provide sufficient omics data for yak fertilization, which is also of great significance to altitude medicine.

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Cloning and expression analysis of the follicle-stimulating hormone receptor (FSHR) gene in the reproductive axis of female yaks (Bos grunniens)

Cited by 12 publications

References 48 publications

CYP19A1 May Influence Lambing Traits in Goats by Regulating the Biological Function of Granulosa Cells

CYP19A1 May Influence Lambing Traits in Goats by Regulating the Biological Function of Granulosa Cells

Detection of Selection Signals in Cattle Populations by Pca

Identification of key genes and biological pathways in different parts of yak oviduct based on transcriptome analysis

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