Cloning and expression of a putative transferrin cDNA of the spruce budworm, Choristoneura fumiferana

Ampasala, Dinakar R.; Zheng, Sichun; Retnakaran, Arthur; Krell, Peter J.; Arif, Basil M.; Feng, Qili

doi:10.1016/j.ibmb.2004.03.002

Cited by 26 publications

(37 citation statements)

References 30 publications

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“…Transferrin, in particular, has been identified as an immune-responsive molecule following microbial challenge (Yoshiga et al, 1997b, Yoshiga et al, 1999, Yun et al, 1999, Thompson et al, 2003a, Beerntsen et al, 1994, Ampasala et al, 2004. The identification of motifs associated with the binding of the transcriptional control element, NF-κB, identified in the Ae.…”

Section: Discussionmentioning

confidence: 99%

Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Strickler-Dinglasan

Güz

Attardo

et al. 2006

Insect Biochemistry and Molecular Biology

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The regulation of iron is critical for maintaining homeostasis in the tsetse fly (Diptera:Glossinidae), in which both adult sexes are strict blood feeders. We have characterized the cDNAs for two putative iron-binding proteins (IBP) involved in transport and storage; transferrin (GmmTsf1) and ferritin from Glossina morsitans morsitans. GmmTsf1 transcripts are detected in the female fat body and in adult reproductive tissues, and only in the adult developmental stage in a bloodmeal independent manner. In contrast, the ferritin heavy chain (GmmFer1HCH) and light chain (GmmFer2LCH) transcripts are expressed ubiquitously, suggesting a more general role for these proteins in iron transport and storage. Protein domain predictions for each IBP suggest both the conservation and loss of several motifs present in their vertebrate homologues. In concert with many other described insect transferrins, putative secreted GmmTsf1 maintains 3 of the 5 residues necessary for ironbinding in the N-terminal lobe, but exhibits a loss of this iron-binding ability in the C-terminal role as well as a loss of large sequence blocks. Both putative GmmFer1HCH and GmmFer2LCH proteins have signal peptides, similar to other insect ferritins. GmmFer2LCH has lost the 5'UTR ironresponsive element (IRE) and, thus, translation is no longer regulated by cellular iron levels. On the other hand, GmmFer1HCH maintains both the conserved ferroxidase center and the 5'UTR IRE; however, transcript variants suggest a more extensive regulatory mechanism for this subunit.

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Section: Discussionmentioning

confidence: 99%

Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Strickler-Dinglasan

Güz

Attardo

et al. 2006

Insect Biochemistry and Molecular Biology

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“…The predicted mass of Tsf1 of B. mori (Lepidoptera) is 73.8 kDa (Table 1a; [70]), while that of Choristoneura fumiferana (ChfTsf1; Lepidoptera; spruce budworm) is 73.9 kDa (Table 1a; [81]). Both sequences show high identity to MsTsf1 (Table 2) and have predicted glycosylation sites [81], as does Tsf1 from Chilo suppressalis (CsTsf1; Lepidoptera; striped riceborer; [82]).…”

Section: Features Of Genomic and Primary Structurementioning

confidence: 99%

Insect transferrins: Multifunctional proteins

Geiser

Winzerling

2012

Biochimica et Biophysica Acta (BBA) - General Subjects

115

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“…Transcription and translation of the transferrin protein are upregulated by bacterial infection in several species of insects, including Lepidoptera (Nichol et al, 2002;Seitz et al, 2003;Ampasala et al, 2004;Yun et al, 2009;Guz et al, 2012). Here we demonstrate that SpliTrf is also upregulated in response to challenge with a gram-positive bacterium, B. thuringiensis.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, it is known that transferrin synthesis is increased following exposure to bacteria, fungi, pathogens, and parasites, as well as insect parasitoids (Beernsten et al, 1994;Yoshiga et al, 1997Yoshiga et al, , 1999Kucharski et al, 2003;Thompson et al, 2003;Ampasala et al, 2004;Valles et al, 2005;Bergin et al, 2006;Lee et al, 2006;Guz et al, 2007Guz et al, , 2012Magalhaes et al, 2007;Paily et al, 2007;Wang et al, 2007;Mowlds et al, 2008;Yun et al, 2009;Zhou et al, 2009;Kim et al, 2010). Interestingly, although transferrin expression was found to be induced by dengue virus infection of the salivary glands in yellow fever mosquito, Aedes aegypti (Luplertlop et al, 2011), transferrin protein levels were shown to be downregulated (Tchankouo et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis

Güz¹,

Dageri²,

Erdoğan³

et al. 2013

Turk J Biol

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Insect immune responses involved in virus defense have lately received increasing attention. While the pathways and effector molecules active in defense against bacteria and fungi are well studied (Ferrandon et al., 2007;Levitin et al., 2008), the regulation of the innate immune system against viral infections still remains to be elucidated. The baculoviruses (Baculoviridae) are doublestranded DNA viruses that infect arthropods, mainly insects and in particular Lepidoptera (Cory and Myers, 2003). Baculoviruses are not only biocontrol agents of lepidopteran pests, but also have been developed and used extensively in research, serving as expression vectors for high-level production of recombinant proteins (Bonning and Hammock, 1996;Susurluk et al., 2013). Although baculoviruses have been successfully used to control lepidopteran and hymenopteran insect pests of agriculture and forestry importance worldwide, little is known about the host immune responses towards these viral infections (Federici, 1986;Miller, 1997; Moscardi, 1999).Transferrin is an iron-binding protein that has a role in iron transport (Nichol et al., 2002), in preventing oxidative stress, and in delivering iron to eggs for development (Yoshiga et al., 1997). In addition, it is known that transferrin synthesis is increased following exposure to bacteria, fungi, pathogens, and parasites, as well as insect parasitoids (

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Cloning and expression of a putative transferrin cDNA of the spruce budworm, Choristoneura fumiferana

Cited by 26 publications

References 30 publications

Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Molecular characterization of iron binding proteins from Glossina morsitans morsitans (Diptera: Glossinidae)

Insect transferrins: Multifunctional proteins

Transcriptional profiling of transferrin gene from Egyptian cotton leaf worm, Spodoptera littoralis

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