2007
DOI: 10.1271/bbb.60703
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Cloning and Expression of a Novel Sulfotransferase with Unique Substrate Specificity fromBombyx mori

Abstract: We identified a cDNA encoding a putative cytosolic sulfotransferase (SULT) by searching the expressed sequence tag database of Bombyx mori, and subsequently obtained the full-length cDNA for this gene via rapid amplification of cDNA ends (RACE). We designated this gene bmST1, and showed by sequence analysis that it belongs to a novel SULT family. The tissue specificity of bmST1 mRNA expression was examined in fifth instar larvae by reverse transcriptase-polymerase chain reaction (RT-PCR), and transcripts were … Show more

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Cited by 16 publications
(14 citation statements)
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“…A few of these non-mammalian sulfotransferases have been characterized including bmST ( Bombyx mori , silkworm, Uniprot a0pcf9_bommo), dmST1-4 ( Drosophila melanogaster , fruitfly), and that from Spodoptera frugiperda (fall army worm, Uniprot q26490_spofr). The sulfotransferase isolated from Bombyx mori was found to catalyze sulfonation of 4-nitrocatechol although no endogenous substrate has yet been identified (9). The Spodoptera frugiperda enzyme sulfonates retinol (10).…”
Section: Resultsmentioning
confidence: 99%
“…A few of these non-mammalian sulfotransferases have been characterized including bmST ( Bombyx mori , silkworm, Uniprot a0pcf9_bommo), dmST1-4 ( Drosophila melanogaster , fruitfly), and that from Spodoptera frugiperda (fall army worm, Uniprot q26490_spofr). The sulfotransferase isolated from Bombyx mori was found to catalyze sulfonation of 4-nitrocatechol although no endogenous substrate has yet been identified (9). The Spodoptera frugiperda enzyme sulfonates retinol (10).…”
Section: Resultsmentioning
confidence: 99%
“…The SULT activities of the purified recombinant dmSTs were determined using [ 35 S]PAPS as the sulfate donor and the indicated compounds as sulfate acceptors, as described previously. 9) Briefly, under typical assay conditions, the reaction mixtures (250 ml) contained 50 mM sodium phosphate, pH 6.8, 1 mM [ 35 S]PAPS (3.7 kBq), and 100 mM acceptors. These mixtures were incubated at 30 C for 30 min and the reaction was stopped in each case by the addition of 100 ml of 1:1 mixture of 0.1 M barium acetate and barium hydroxide.…”
Section: Methodsmentioning
confidence: 99%
“…[4][5][6] Various SULT genes are also registered in the genome databases of invertebrates, but with the exception of SULT101A1, ceST1, and bmST1, their enzymatic characteristics remain to be elucidated. [7][8][9] SULT101A1 was originally purified from Spodoptera frugiperda and designated a retinol dehydratase, based on its enzymatic activity. This gene was later classified as a SULT family member by amino acid sequence alignment.…”
mentioning
confidence: 99%
“…We have previously characterized novel genes in the silkworm using sequence homology comparison and recombinant expression studies. We have demonstrated successful studies, such as the cloning, expression and localization study of a novel Sulfotransferase gene from Bombyx mori [7], the determination of a novel lebocinlike gene from eri-silkworm [8], a novel cuticle protein gene BmCPG1 in the silkworm with Ecdysteroid-dependent expression [9], and a Bombyx mori gene Bmchi-h encoding a protein was found which is homologous to bacterial and baculovirus chitinases [10,11]. So far, there have been few studies of the silkworm novel genes which lack previous understanding and have low homology with any known genes.…”
Section: Introductionmentioning
confidence: 99%