Cloning and expression of a human pro(tea)some β‐subunit cDNA: A homologue of the yeast PRE4‐subunit essential for peptidylglutamyl‐peptide hydrolase activity

Proteasomes are protein complexes that mediate controlled degradation of damaged or unneeded cellular proteins. In neurons, proteasome regulates synaptic function and its dysfunction has been linked to neurodegeneration and neuronal cell death. However, endogenous mechanisms controlling proteasomal activity are insufficiently understood. Here, we describe a novel interaction between presynaptic scaffolding protein bassoon and PSMB4, a β subunit of the 20S core proteasome. Expression of bassoon fragments that interact with PSMB4 in cell lines or in primary neurons attenuates all endopeptidase activities of cellular proteasome and induces accumulation of several classes of ubiquitinated and non-ubiquitinated substrates of the proteasome. Importantly, these effects are distinct from the previously reported impact of bassoon on ubiquitination and autophagy and might rely on a steric interference with the assembly of the 20S proteasome core. In line with a negative regulatory role of bassoon on endogenous proteasome we found increased proteasomal activity in the synaptic fractions prepared from brains of bassoon knock-out mice. Finally, increased activity of proteasome and lower expression levels of synaptic substrates of proteasome could be largely normalized upon expression of PSMB4-interacting fragments of bassoon in neurons derived from bassoon deficient mice. Collectively, we propose that bassoon interacts directly with proteasome to control its activity at presynapse and thereby it contributes to a compartment-specific regulation of neuronal protein homeostasis. These findings provide a mechanistic explanation for the recently described link of bassoon to human diseases associated with pathological protein aggregation. Graphic Abstract Presynaptic cytomatrix protein bassoon (Bsn) interacts with PSMB4, the β7 subunit of 20S core proteasome, via three independent interaction interfaces. Bsn inhibits proteasomal proteolytic activity and degradation of different classes of proteasomal substrates presumably due to steric interference with the assembly of 20S core of proteasome. Upon Bsn deletion in neurons, presynaptic substrates of the proteasome are depleted, which can be reversed upon expression of PSMB4-interacting interfaces of Bsn. Taken together, bsn controls the degree of proteasome degradation within the presynaptic compartment and thus, contributes to the regulation of synaptic proteome

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Combined effects of gamma radiation and arsenite on the proteome of human TK6 lymphoblastoid cells

Tapio

Danescu-Mayer

Asmuss

et al. 2005

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Cloning and expression of a human pro(tea)some β‐subunit cDNA: A homologue of the yeast PRE4‐subunit essential for peptidylglutamyl‐peptide hydrolase activity

Cited by 11 publications

References 19 publications

Genetic Relationships of the Genes Encoding the Human Proteasome β Subunits and the Proteasome PA28 Complex

Genetic Relationships of the Genes Encoding the Human Proteasome β Subunits and the Proteasome PA28 Complex

Bassoon inhibits proteasome activity via interaction with PSMB4

Combined effects of gamma radiation and arsenite on the proteome of human TK6 lymphoblastoid cells

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