Cloning and expression of a novel cDNA encoding a mannose-specific jacalin-related lectin from Oryza sativa

Jiang, Jiafu; Han, Ye; Xing, Lijing; Xu, Yunyuan; Xu, Zhi-Hong; Chong, Kang

doi:10.1016/j.toxicon.2005.10.010

Cited by 59 publications

(61 citation statements)

References 24 publications

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“…NM_112518). This last result is in accordance with those reported in recent works using transcriptional approaches (Jiang et al, 2006;Sarosh and Meijer, 2007). Indeed, in oilseed rape, Sarosh and Meijer (2007) have shown that MeJA induced the gene expression of Jacalin-related lectins that have a role in plant defense pathway.…”

Section: Identification Of Other Proteins Highly Induced By Mejasupporting

confidence: 92%

A Proteomic Profiling Approach to Reveal a Novel Role of Brassica napus Drought 22 kD/Water-Soluble Chlorophyll-Binding Protein in Young Leaves during Nitrogen Remobilization Induced by Stressful Conditions

et al. 2008

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Despite its water-soluble chlorophyll-binding protein (WSCP) function, the putative trypsin inhibitor (TI) activity of the Brassica napus drought 22 kD (BnD22) protein and its physiological function in young leaves during leaf nitrogen (N) remobilization promoted by stressful conditions remains an enigma. Therefore, our objectives were to determine (1) if BnD22 is related to the 19-kD TI previously detected in B. napus young leaves, and (2) if the levels of BnD22 transcripts, BnD22 protein, and TI activity in young leaves are associated with plant responses to stress conditions (N starvation and methyl jasmonate [MeJA] treatments) that are able to modulate leaf senescence. Compared to control, N starvation delayed initiation of senescence and induced 19-kD TI activity in the young leaves. After 3 d with MeJA, the 19-kD TI activity was 7-fold higher than the control. Using two-dimensional electrophoresis gel, TI activity, and electrospray ionization liquid chromatography tandem mass spectrometry analysis, it was demonstrated that two 19-kD proteins with isoelectric points 5.0 and 5.1 harboring TI activity correspond to BnD22 perfectly. BnD22 gene expression, TI activities, and BnD22 protein presented similar patterns. Using polyclonal anti-WSCP antibodies of Brassica oleracea, six polypeptides separated by two-dimensional electrophoresis were detected in young leaves treated with MeJA. Electrospray ionization liquid chromatography tandem mass spectrometry analysis of six polypeptides confirms their homologies with WSCP. Results suggest that BnD22 possesses dual functions (WSCP and TI) that lead to the protection of younger tissues from adverse conditions by maintaining metabolism (protein integrity and photosynthesis). By sustaining sink growth of stressed plants, BnD22 may contribute to a better utilization of recycling N from sources, a physiological trait that improves N-use efficiency.

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Section: Identification Of Other Proteins Highly Induced By Mejasupporting

confidence: 92%

A Proteomic Profiling Approach to Reveal a Novel Role of Brassica napus Drought 22 kD/Water-Soluble Chlorophyll-Binding Protein in Young Leaves during Nitrogen Remobilization Induced by Stressful Conditions

et al. 2008

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“…70,72 Several jasmonate-inducible lectins are expressed in leaf tissues of monocots such as rice, barley, wheat, rye, and maize. 73 Advancement of our understanding in induction of plant lectins in response to various stresses, especially herbivory, and their role in plant defense has the potential for utilization of these entomotoxic lectins in crop protection through genetic engineering. Although, transformation of lectin genes into plants seems to be very attractive and effective, care is needed, because of possible toxicity of some lectins to non-target organisms, including mammals.…”

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confidence: 99%

Mechanisms of plant defense against insect herbivores

War

Paulraj

Ahmad

et al. 2012

Plant Signaling & Behavior

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“…As a subgroup of lectins, Jacalin-lectin proteins have one or more jacalin-like lectin domains with binding specificity to Man or Gal (Kabir, 1998;Peumans et al, 2001). They are involved in multiple biological processes (Aucouturier et al, 1989;Yong et al, 2003;Jiang et al, 2006). Arabidopsis (Arabidopsis thaliana) JASMONATE ACID RESPONSE1 and wheat (Triticum aestivum) JACALIN-RELATED LECTIN LIKE1 are involved in jasmonic acid-mediated defense responses (León et al, 1998;Xiang et al, 2011).…”

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confidence: 99%

AtJAC1 Regulates Nuclear Accumulation of GRP7, Influencing RNA Processing of FLC Antisense Transcripts and Flowering Time in Arabidopsis

Xiao

et al. 2015

Plant Physiol.

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Lectins selectively recognize sugars or glycans for defense in living cells, but less is known about their roles in the development process and the functional network with other factors. Here, we show that Arabidopsis (Arabidopsis thaliana) JACALIN-LECTIN LIKE1 (AtJAC1) functions in flowering time control. Loss of function of AtJAC1 leads to precocious flowering, whereas overexpression of AtJAC1 causes delayed flowering. AtJAC1 influences flowering through regulation of the key flowering repressor gene FLOWERING LOCUS C (FLC). Genetic analysis revealed that AtJAC1's function is mostly dependent on GLYCINE-RICH RNA-BINDING PROTEIN7 (GRP7), an upstream regulator of FLC. Biochemical and cell biological data indicated that AtJAC1 interacted physically with GRP7 specifically in the cytoplasm. AtJAC1 influences the nucleocytoplasmic distribution of GRP7, with predominant nuclear localization of GRP7 when AtJAC1 function is lost but retention of GRP7 in the cytoplasm when AtJAC1 is overexpressed. A temporal inducible assay suggested that AtJAC1's regulation of flowering could be compromised by the nuclear accumulation of GRP7. In addition, GRP7 binds to the antisense precursor messenger RNA of FLC through a conserved RNA motif. Loss of GRP7 function leads to the elevation of total FLC antisense transcripts and reduced proximal-distal polyadenylation ratio, as well as histone methylation changes in the FLC gene body region and increased total functional sense FLC transcript. Attenuating the direct binding of GRP7 with competing artificial RNAs leads to changes of FLC antisense precursor messenger RNA processing and flowering transition. Taken together, our study indicates that AtJAC1 coordinates with GRP7 in shaping plant development through the regulation of RNA processing in Arabidopsis.

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Cloning and expression of a novel cDNA encoding a mannose-specific jacalin-related lectin from Oryza sativa

Cited by 59 publications

References 24 publications

A Proteomic Profiling Approach to Reveal a Novel Role of Brassica napus Drought 22 kD/Water-Soluble Chlorophyll-Binding Protein in Young Leaves during Nitrogen Remobilization Induced by Stressful Conditions

A Proteomic Profiling Approach to Reveal a Novel Role of Brassica napus Drought 22 kD/Water-Soluble Chlorophyll-Binding Protein in Young Leaves during Nitrogen Remobilization Induced by Stressful Conditions

Mechanisms of plant defense against insect herbivores

AtJAC1 Regulates Nuclear Accumulation of GRP7, Influencing RNA Processing of FLC Antisense Transcripts and Flowering Time in Arabidopsis

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