Cloning and Expression of an Endo-1,6-β-D-glucanase Gene (neg1) fromNeurospora crassa

Free Oligosaccharides to Monitor Glycoprotein Endoplasmic Reticulum-associated Degradation in Saccharomyces cerevisiae

“…The underlined bases in the primer sequences indicate NheI and XhoI sites, respectively. The amplified 1412-base pair fragment, which excluded the signal sequence (44), was digested with NheI and XhoI and then purified. The purified fragment was ligated into the equivalent site of the vector pET28a (ϩ) (Novagen) to generate pHI-NCNEG1 (His 6 -Neg1 expression vector).…”

Senescence of the Lentinula edodes Fruiting Body After Harvesting

“…Lichen Umbilicaria species produce a linear glucan composed of only β-1,6-linkages (pustulan; Nishikawa et al, 1970). Many fungi are considered to secrete β-1,6-glucanases, some of which have been purified and characterized (Bryant et al, 2007;Oyama et al, 2002;Moy et al, 2002;Pitson et al, 1996). All of the isolated enzymes are β-1,6-glucan endohydrolases (EC 3.2.1.75) classified into GH family 5 or 30 of the GH-A clan in the CAZy database.…”

The β-1,3-Exoglucanase GeneexgA(exg1) ofAspergillus oryzaeIs Required to Catabolize Extracellular Glucan, and Is Induced in Growth on a Solid Surface

“…The wild-type strain, the exgA-overexpressing mutant, and the exgA disruptant were inoculated on CD agar supplemented with SDS or Congo red, which are known to inhibit -glucan remodeling, 21) at a concentration of 0 or 0.005% for SDS and 0, 0.0025, or 0.025% for Congo red. The growth rate and morphology of the exgA disruptant, the exgA-overexpressing mutant, and the wild-type strain were indistinguishable in all the culture conditions above.…”