Cloning and expression of candidate allergens from Culicoides obsoletus for diagnosis of insect bite hypersensitivity in horses

Meide, Nathalie M.A. van der; Roders, Nathalie; Oldruitenborgh‐Oosterbaan, M.M. Sloet van; Schaap, Peter J.; Oers, Monique M. van; Leibold, W.; Savelkoul, H.F.J.; Tijhaar, Edwin

doi:10.1016/j.vetimm.2013.03.005

Cited by 45 publications

(37 citation statements)

References 31 publications

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“…[2][3][4][5][6] These recombinant allergens (r-allergens) bind serum IgE from IBH-affected animals, but only rarely from healthy control horses. [3][4][5][6][7] Affected horses have been shown to react to antigens of many different indigenous, as well as nonindigenous Culicoides spp. in intradermal tests, 8,9 therefore suggesting shared common allergens between species.…”

Section: Introductionmentioning

confidence: 99%

Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Ziegler

Hamza

Jónsdóttir

et al. 2017

Veterinary Dermatology

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Background -Insect bite hypersensitivity (IBH) is an allergic dermatitis of horses caused by bites of Culicoides spp. IBH does not occur in Iceland because of the absence of Culicoides, but the prevalence is high in horses imported from Iceland to environments where Culicoides are present.Hypothesis/Objective -Test, in a longitudinal study before and after Culicoides exposure, whether a primary sensitizing Culicoides allergen can be identified and if an increase of allergen-specific immunoglobulin (Ig)E or IgG subclasses precedes clinical signs of IBH.Animals -Thirty two horses imported from Iceland to Europe; 16 developed IBH and 16 remained healthy. Methods -Determination of IgE and IgG subclasses against recombinant (r)-Culicoides allergens andCulicoides extract in sera taken before first exposure to Culicoides and yearly over a period of 3-4 years.Results -Before Culicoides exposure, there were no significant differences in Culicoides-specific serum IgE levels between horse that developed IBH or remained healthy. Culicoides exposure induced an individual IgE response pattern (to a median of 4.5 r-allergens) in the IBH but not in the healthy end-point group. The increase in serum IgE levels to Culicoides r-allergens was concurrent with the initial onset of clinical signs of IBH. IBHaffected horses displayed significantly higher allergen-specific IgG1 and IgG5 levels than healthy controls. Recombinant Culicoides obsoletus 1 (Cul o1) and Cul o3-specific IgG5 was significantly higher in the IBH compared to the healthy end-point group, before clinical signs of IBH.Conclusion/Clinical relevance -Allergen-specific serum IgE cannot be used as predictor for IBH, whereas allergen-specific IgG5 levels may have a predictive value.

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Section: Introductionmentioning

confidence: 99%

Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Ziegler

Hamza

Jónsdóttir

et al. 2017

Veterinary Dermatology

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“…However, C. nubeculosus is not the most abundant species of Culicoides found biting horses (91,92), and higher levels of IgE antibody are detected when extracts from Culicoides obsoletus are used in diagnostic testing (86,93). This finding has been confirmed using expressed recombinant C. obsoletus protein antigens in an IgE specific ELISA (94,95).…”

Section: Major Allergensmentioning

confidence: 62%

“…Despite this progress in identifying the major IBH allergens, significant gaps remain; in particular, several C. obsoletus proteins of around 40kD in mass, which react with IgE in allergic horse serum, have not been identified (95), and as yet no homologues of the major C. nubeculosus antigens of the CNSG60, CNSG79, Cul n 3,5&10 family (88, 90) have been found in C. obsoletus libraries although several candidate proteins of about this mass are present as have several C. obsoletus and Culicoides pulicaris salivary gland cDNA sequences encoding novel secretory proteins (A. D. Wilson, unpublished).…”

Section: Major Allergensmentioning

confidence: 99%

Immune responses to ectoparasites of horses, with a focus on insect bite hypersensitivity

Wilson

2014

Parasite Immunology

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Horses are affected by a wide variety of arthropod ectoparasites, ranging from lice which spend their entire life on the host, through ticks which feed over a period of days, to numerous biting insects that only transiently visit the host to feed. The presence of ectoparasites elicits a number of host responses including innate inflammatory responses, adaptive immune reactions and altered behaviour; all of which can reduce the severity of the parasite burden. All of these different responses are linked through immune mechanisms mediated by mast cells and IgE antibodies which have an important role in host resistance to ectoparasites, yet immune responses also cause severe pathological reactions. One of the best described examples of such pathological sequelae is insect bite hypersensitivity (IBH) of horses; an IgE-mediated type 1 hypersensitivity to the salivary proteins of Culicoides spp. associated with T-helper-2 production of IL4 and IL13. Importantly, all horses exposed to Culicoides have an expanded population of Culicoides antigen-specific T cells with this pattern of cytokine production, but in those which remain healthy, the inflammatory reaction is tempered by the presence of FoxP3+ CD4+ regulatory T cells that express IL10 and TGF-beta, which suppresses the IL4 production by Culicoides antigen-activated T cells.

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“…The midges are not found in Iceland and therefore the disease does not occur there [1]. It has been isolated from Culicoides nubeculosus (Cul n 2) [4] and Culicoides obsoletus (Cul o 2) [5]. The causative allergens of insect bite hypersensitivity originate in the salivary glands of Culicoides spp.…”

Section: Introductionmentioning

confidence: 99%

Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response

Jónsdóttir

Svansson

Stefánsdóttir

et al. 2016

Equine Veterinary Journal

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Cloning and expression of candidate allergens from Culicoides obsoletus for diagnosis of insect bite hypersensitivity in horses

Cited by 45 publications

References 31 publications

Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses

Immune responses to ectoparasites of horses, with a focus on insect bite hypersensitivity

Oral administration of transgenic barley expressing a Culicoides allergen induces specific antibody response

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