Cloning and expression of canine CD25 for validation of an anti-human CD25 antibody to compare T regulatory lymphocytes in healthy dogs and dogs with osteosarcoma

All creatures great and small: regulatory T cells in mice, humans, dogs and other domestic animal species

“…Cross-reactivity of the anti-human CD25 mAb ACT-1 with the canine protein was also confirmed using the same strategy [302], supporting earlier work showing positive staining of activated canine T cells with ACT-1 [303]. Subsequent studies have verified cross-reactivity of ACT-1 for canine CD25 [304], but have suggested that the affinity of this antibody for the canine protein is low [305]; indeed, Abrams and colleagues have developed a novel, higher-affinity, canine-specific anti-CD25 mAb (P4A10), which is now commercially available [305].…”

“…Further work performed by the same group demonstrated increased proportions of CD4 + FOXP3 + T cells in the peripheral blood and tumour beds of canine oral malignant melanoma patients [308]; furthermore, the proportion of peripheral blood CD4 + FOXP3 + T cells showed a positive correlation with tumour stage and negative correlations with the proportions of Th1 and cytotoxic T cells in these animals [309]. However, not all studies of canine tumours have yielded such a clear message: for example, Rissetto and colleagues found no difference in the proportions of CD4 + FOXP3 + cells in the peripheral blood or lymph nodes -either regional or distant -in dogs with osteosarcoma [304], yet Biller and colleagues documented increased proportions of these cells in the blood, but not lymph nodes, of dogs with the same type of tumour, with an associated decrease in both the proportion of CD8 + T cells and the ratio of CD8 + : CD4 + FOXP3 + cells [310]; indeed, these authors also demonstrated that CD8 + : CD4 + FOXP3 + ratios lower than the mean value in the patient group were associated with significantly shorter survival times, raising the intriguing possibility that the Tregs in these dogs were implicated in the immune evasion of the cancer [310]. Studies interrogating CD4 + FOXP3 + /CD25 + T cells or FOXP3 expression in other areas of canine medicine have been scant.…”

A Interleucina-10 E Seu Papel Nos Carcinomas Mamários Caninos

“…A relação direta entre a intensidade da inflamação e a imunomarcação para IL-10 sugere que o aumento na presença de linfócitos está vinculado a um aumento na presença de linfócitos T regulatórios (Tregs) no sítio tumoral, causando um incremento no infiltrado inflamatório, mas tornando-o imunologicamente comprometido. Os linfócitos Tregs são um constituinte particular da população de linfócitos T auxiliares (CD4+), que se diferenciam em Tregs na presença de IL-10 e suprimem a resposta imune contra o tumor (RISSETO et al, 2010). Esses efeitos supressivos têm ação direta sobre os linfócitos T CD4+, resultando em uma ineficiente ativação da imunidade celular.…”

Regulatory T Cells and Their Role in Animal Disease

“…However, in a study that compared dogs with osteosarcoma to healthy dogs, no significant difference in frequencies of Tregs was reported. 60 The authors hypothesized that discrepancies between their study and the previously described studies could be due to a small sample size, use of submandibular lymph nodes (proximity to the oral cavity could promote immune reactivity that could affect Treg percentage), and the possibility that not all Tregs were functional. The use of different antibodies to identify Tregs could also explain these differences.…”