Cloning and Expression of DNA Coding for the Major House Dust Mite Allergen <i>Der p</i> 1 in <i>Escherichia coli</i>

Abstract

A cDNA clone coding for the major house dust mite allergen Der p 1 was isolated from a lambda gt 11 library. Its sequence correlates with known amino acid sequences of Der p 1 and it produces a fusion protein which reacts with rabbit anti-Der p 1 antiserum.

Recombinant Allergens in Structural Biology, Diagnosis, and Immunotherapy

“…In January 1988, the cDNA sequence coding for the major house dust mite allergen Der p 1 was discovered in Australia and published as the first complete allergen sequence worldwide [1]. Interestingly, the publication of the identification of the λgt11 clone expressing Der p 1 was only published later that year [2]. The sequence of a major allergen of the white-faced hornet Dolichovespula maculata , then named antigen 5 and now designated as Dol m 5, was cloned in February 1988 in New York City and published by Fang et al [3].…”

“…Der p 1 was identified by screening the λgt11 cDNA expression library with a rabbit anti-Der p 1 antiserum [2]. Dol m 5 was discovered by screening a λgt11 cDNA expression library with hornet antigen 5-specific mouse sera [3].…”

Antigenic Analyses of Sugi Basic Protein by Monoclonal Antibodies: II. Detection of Immunoreactive Fragments in Enzyme-cleaved <i>Cry j l</i>

“…Mapping of allergenic epitopes on some allergens by using mAbs has been well done as re ported on grass pollen allergens [2][3][4][5][6][7], house dust mite al lergens [8][9][10][11][12][13][14], cod fish allergen M [15] and some insect al lergens [16,17]. Recently, the complete primary structures of a white-face hornet venom allergen [17], a house dust mite allergen Derp I [18,19] and of the IgE-binding protein from Kentucky bluegrass pollen [20] were determined. However, in a few cases, the information on the structures of the epitopes on these allergens is available [5,15,16,20], but not in Japanese cedar pollen allergens.…”

Isolation and in vitro Translation of mRNA from Inflorescences of <i>Parietaria judaica</i>

“…Recently, some authors have used the methodol ogy of recombinant DNA to obtain allergens synthe sized in bacteria; the cDNA copies of the genes for major allergens of the house dust mite Dermatophagoides pteronvssinus, a 27 kD protein, and of the venom of the white-face hornet, Dolichovespula maculata, a 23 kD protein, have been cloned and expressed in Escherichia coli [10][11][12][13]. The availability of Paro 1 synthesized in E. coli may allow approaches to the an alysis of the physicochemical structure of this allergen complementary to those afforded by the traditional methodology of protein biochemistry.…”