Cloning and expression of gellan gum biosynthetic genes gelQ, gelB, gelL and gel K of Sphingomonas paucimobilis, production and characterization of the recombinant gellan gum

Manjusha, C. M.; Soumiya, S; Santhiagu, A.

doi:10.1016/j.bcab.2020.101850

Cited by 7 publications

(3 citation statements)

References 21 publications

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“…High acyl GG can form a flexible and soft gel with a fibrous gel network due to the presence of a glycerate group. [ 269–275 ] GG and GG‐coated materials find applications in various corneal tissue engineering approaches, including ocular surface drugs, topical ocular administration, and ocular pharmacotherapy. [ 276 ]…”

Section: Natural Biomaterials For Corneal Tissue Engineeringmentioning

confidence: 99%

“…High acyl GG can form a flexible and soft gel with a fibrous gel network due to the presence of a glycerate group. [269][270][271][272][273][274][275] GG and GG-coated materials find applications in various corneal tissue engineering approaches, including ocular surface drugs, topical ocular administration, and ocular pharmacotherapy. [276] GG is an FDA-approved ingredient found in several commercial products, including Gelrite®, GelGro®, Kelcogel®, and Phytagel®.…”

Section: Gellan Gum-based Biomaterials For Corneal Tissue Engineeringmentioning

confidence: 99%

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The Design and Developments of Protein‐Polysaccharide Biomaterials for Corneal Tissue Engineering

Prasathkumar

Chenthamara

Lin

et al. 2023

Adv Materials Technologies

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Corneal blindness is explained by a large reduction in vision loss caused by acute and chronic corneal diseases and/or disorders. Due to the severe unavailability of donor corneas, immune rejection, and the side effects of keratoprosthesis (KPro), vision‐impaired patients are often unable to restore their vision. Corneal tissue engineering appears to be a promising method using natural polymers made from different layers of corneal cells such as epithelium, stroma, and endothelium or full tissue equivalents. Proteins and polysaccharides are able to mimic the chemical composition of the native extracellular matrix (ECM), which has been extensively studied for corneal tissue engineering. Additionally, protein‐based materials are highly favored in corneal regenerative therapy as they have the proper cell‐binding sites for cell adhesion, corneal innervation regeneration, and oxidative stress suppression, while polysaccharide‐based materials can also promote cell proliferation and adhesion as well as ocular permeability. Herein, a comprehensive review of various biomaterials based on natural proteins (fibrin, fibrinogen, silk fibroin, collagen, gelatin, and serum albumin) and polysaccharides (chitin, chitosan, hyaluronic acid, alginate, cellulose, and gellan gum) for corneal tissue engineering are provided. The protein‐polysaccharide biomaterials are mainly used in the form of hydrogels, films, scaffolds, sutures, eye drops, nanocarriers, and nanocapsules to treat ocular diseases that cause blindness, such as corneal wounds, corneal ulcers, Goldenhar syndrome, corneal endothelium and stroma defects, corneal neovascularization, etc. In this review, insight is provided into recent developments in the areas of biomaterial design, cross‐linking strategy, corneal cell selection, and frontiers that are expected to offer researchers support and creativity in the development of corneal regenerative medicine.

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Section: Natural Biomaterials For Corneal Tissue Engineeringmentioning

confidence: 99%

Section: Gellan Gum-based Biomaterials For Corneal Tissue Engineeringmentioning

confidence: 99%

The Design and Developments of Protein‐Polysaccharide Biomaterials for Corneal Tissue Engineering

Prasathkumar

Chenthamara

Lin

et al. 2023

Adv Materials Technologies

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“…In the effort to enhance gellan production, Manjusha et al [ 47 ] successfully cloned four gellan gum genes ( gelQ , gelK , gelL , and gelB ) and produced the recombinant polysaccharide that was similar in structural properties with the native gellan. Transformation of the recombinant pBBR122- gelD plasmid into Sphingomonas paucimobilis cells and overexpression of the gelD in recombinant strain yielded twice as much gellan in comparison to a wild-type strain [ 48 ].…”

Section: Biosynthesis Of Xanthan and Gellanmentioning

confidence: 99%

Prospects of Biogenic Xanthan and Gellan in Removal of Heavy Metals from Contaminated Waters

et al. 2022

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Biosorption is considered an effective technique for the treatment of heavy-metal-bearing wastewaters. In recent years, various biogenic products, including native and functionalized biopolymers, have been successfully employed in technologies aiming for the environmentally sustainable immobilization and removal of heavy metals at contaminated sites, including two commercially available heteropolysaccharides—xanthan and gellan. As biodegradable and non-toxic fermentation products, xanthan and gellan have been successfully tested in various remediation techniques. Here, to highlight their prospects as green adsorbents for water decontamination, we have reviewed their biosynthesis machinery and chemical properties that are linked to their sorptive interactions, as well as their actual performance in the remediation of heavy metal contaminated waters. Their sorptive performance in native and modified forms is promising; thus, both xanthan and gellan are emerging as new green-based materials for the cost-effective and efficient remediation of heavy metal-contaminated waters.

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Immunomodulatory functions and properties of gellan gum–based formulation

Askari,

Zendedel,

Baradaran Rahimi

2024

Application of Gellan Gum as a Biomedical Polymer

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Cloning and expression of gellan gum biosynthetic genes gelQ, gelB, gelL and gel K of Sphingomonas paucimobilis, production and characterization of the recombinant gellan gum

Cited by 7 publications

References 21 publications

The Design and Developments of Protein‐Polysaccharide Biomaterials for Corneal Tissue Engineering

The Design and Developments of Protein‐Polysaccharide Biomaterials for Corneal Tissue Engineering

Prospects of Biogenic Xanthan and Gellan in Removal of Heavy Metals from Contaminated Waters

Immunomodulatory functions and properties of gellan gum–based formulation

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