Cloning and expression of IGF-I, IGF-II, and GHR genes and the role of their single-nucleotide polymorphisms in the growth of pikeperch (Sander lucioperca)

Teng, Teng; Zhao, Xin; Li, Caijuan; Guo, Jian; Wang, Yunfeng; Pan, Chenglong; Liu, Enguang; Ling, Qufei

doi:10.1007/s10499-020-00542-z

Cited by 9 publications

(7 citation statements)

References 49 publications

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“…The genome structure of igf2 of L. maculatus comprised four exons and three introns ( Figure 2 ). This structure is consistent with that of other fish types, including largemouth bass ( Micropterus salmoides ), common carp ( Cyprinus carpio ), salmon ( Oncorhynchus keta ), tilapia ( Oreochromis niloticus ), Asian sea bass ( Lates calcarifer ), and pike perch ( Sander lucioperca ) [ 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. This result indicates that the structure of the igf2 genome of fish is relatively conserved.…”

Section: Discussionsupporting

confidence: 83%

“…For example, one SNP of igf2 was found to be correlated significantly with nine growth traits of the hybrid yellow catfish ( P. fulvidraco (♀) × Pelteobagrus vachelli (♂)) [ 2 ]. One SNP in intron 3 of igf2 of S. lucioperca is closely related to body weight [ 31 ]. One SNP locus in exon 3 of igf2 in tilapia ( O. niloticus ) is correlated with growth traits [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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Molecular Cloning, Screening of Single Nucleotide Polymorphisms, and Analysis of Growth-Associated Traits of igf2 in Spotted Sea Bass (Lateolabrax maculatus)

Fan

Wang

Zhao

et al. 2023

Animals

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The insulin-like growth factor 2 gene (igf2) is thought to be a key factor that could regulate animal growth. In fish, few researchers have reported on the single nucleotide polymorphisms (SNPs) located in igf2 and their association with growth traits. We screened the SNPs of igf2 from the spotted sea bass (Lateolabrax maculatus) by Sanger sequencing and made an association between these SNPs with growth traits. The full-length complementary (c) DNA of igf2 was 1045 bp, including an open reading frame of 648 bp. The amino acid sequence of Igf2 contained a signal peptide, an IGF domain, and an IGF2_C domain. Multiple sequence alignment showed that the IGF domain and IGF2_C domain were conserved in vertebrates. The genome sequence of igf2 had a length of 6227 bp. Fourteen SNPs (13 in the introns and one in one of the exons) were found in the genome sequence of igf2. Four SNPs located in the intron were significantly associated with growth traits (p < 0.05). These results demonstrated that these SNPs could be candidate molecular markers for breeding programs in L. maculatus.

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Section: Discussionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Molecular Cloning, Screening of Single Nucleotide Polymorphisms, and Analysis of Growth-Associated Traits of igf2 in Spotted Sea Bass (Lateolabrax maculatus)

Fan

Wang

Zhao

et al. 2023

Animals

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“…This decrease in igfI in the juveniles can stop muscle proliferation and development. Teng et al (2020) reported that igfI correlates more with sh growth. In pikeperch from 9 to 14 DPH, the initial feeding rate increased rapidly, and reached to the highest expression level (about 82.7%) at 14 DPH (Xu et al 2017).…”

Section: Discussionmentioning

confidence: 99%

The pattern of gene expression (IGF family, muscle growth regulatory factors and osteogenesis related genes) involved in growth of skeletal muscle in pikeperch (Sander lucioperca) during ontogenesis

Lavajoo,

Falahatkar,

Perelló-Amorós

et al. 2023

Preprint

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The pikeperch (Sander lucioperca) is an economically important freshwater fish and a valuable food with high market acceptance. It is undergoing important changes in growth and regulatory metabolism during the ontogeny. Hence, the current study aims to investigate the mRNA expression of the growth hormone (gh)/insuline-like growth factor (igf) axis (ghr, igfI, igfbp, igfr), muscle regulatory factors (pax7, myf5, myod, myogenin, mrf, mymk, mstn) and osteogenesis related genes (colla1a, fib1a, on, op, ostn) from hatching through day 40th post hatching (DPH). The average total length (TL) of larvae measured at hatching was 3.6 ± 0.4 mm (67 degree-day) and at the end of experiment (40 DPH, 777 degree-day) was 27.1 ± 1.1 mm. The results showed three phases of gene expression in the egg, larval and juvenile stages of pikeperch ontogenesis. The expression pattern of fib1a and muscle gene myf5 showed the highest value at the egg stage. The growth hormone receptor gene (ghr), mymk and mstnb, raised to 1 DPH, whereas the expression of igf1rb and igf1bp5 was increased from 1 to 14 DPH. Increased expression of igfI, igfII, igf1bp4 and mrf4 was detected after 10 and 14 DPH. The myod1 expression raised at 8, 14 and 21 DPH. Differently myod2 showed only a large transient downregulation at 3 DPH and juvenile stage. The six genes igf1ra, pax7, myogenin, col1a1a, on, and ostc were highly expressed at 21 DPH and juvenile stage. Results from this study suggest that basal metabolism, growth of body cells, muscle and bone proliferation and development can be regulated by the dynamic changes in gene expression patterns in this species. The identified genes will help to understand the basic biological process of pikeperch larvae, and development, which is very important in pikeperch farming.

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“…The growth rate is a significant commercial characteristic of aquaculture animals that genotypes and environmental factors can affect [29]. GHR is one of the vital factors for the growth axis of GH/IGF and plays a prominent role in the growth of animals.…”

Section: Discussionmentioning

confidence: 99%

Novel SNPs in the 3′UTR Region of GHRb Gene Associated with Growth Traits in Striped Catfish (Pangasianodon hypophthalmus), a Valuable Aquaculture Species

Jiang

Ruan

et al. 2022

Fishes

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The striped catfish, Pangasianodon hypophthalmus is an important freshwater aquaculture species in Southeast Asian countries due to its rapid growth. The growth hormone receptor (GHR) is a significant regulatory factor for the growth axis and has great potential applications in animal genetic breeding. This study aims to characterize the GHRb cDNA of the striped catfish and analyze the distribution of its mRNA. Screening of single nucleotide polymorphisms’ (SNPs) loci and diplotypes was performed to provide basic information for the assisted selection of molecular markers in genetic breeding. The results showed that the GHRb cDNA of striped catfish had 2791 bp, which encoded for 569 amino acids. In a phylogenyic study, the ghrb of the striped catfish was clustered with those of other catfish and they were highly homologous. Quantitative real-time PCR (qRT-PCR) experiments showed that GHRb mRNA was expressed in ten different organs of the striped catfish, with the highest expression level in the liver. Five SNP and a haplotype block were identified in the 3′UTR of the GHRb gene using the direct sequencing of 307 individuals. Three haplotypes were found and four diplotypes were constructed. The association analysis revealed that these polymorphisms were significantly associated with growth traits in the striped catfish (p < 0.05). These polymorphisms will provide a valuable reference for future molecular genetic marker-assisted breeding of striped catfish.

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Cloning and expression of IGF-I, IGF-II, and GHR genes and the role of their single-nucleotide polymorphisms in the growth of pikeperch (Sander lucioperca)

Cited by 9 publications

References 49 publications

Molecular Cloning, Screening of Single Nucleotide Polymorphisms, and Analysis of Growth-Associated Traits of igf2 in Spotted Sea Bass (Lateolabrax maculatus)

Molecular Cloning, Screening of Single Nucleotide Polymorphisms, and Analysis of Growth-Associated Traits of igf2 in Spotted Sea Bass (Lateolabrax maculatus)

The pattern of gene expression (IGF family, muscle growth regulatory factors and osteogenesis related genes) involved in growth of skeletal muscle in pikeperch (Sander lucioperca) during ontogenesis

Novel SNPs in the 3′UTR Region of GHRb Gene Associated with Growth Traits in Striped Catfish (Pangasianodon hypophthalmus), a Valuable Aquaculture Species

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