Cloning and expression of immunogenic Clostridium botulinum C2I mutant proteins designed from their evolutionary imprints

Prisilla, A.; Chellapandi, P.

doi:10.1016/j.cimid.2019.01.012

Cited by 4 publications

(3 citation statements)

References 48 publications

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“…Interestingly, ComK, CcpC, Hpr, TnrR, IolR, and LevR interacted with putative membrane proteins (CLK_3081) in CBL. Surface membrane proteins are potential vaccine targets for proteolytic and non-proteolytic C. botulinum (Muhammad et al, 2014;Prathiviraj et al, 2016;Prisilla and Chellapandi, 2019;Bhardwaj et al, 2019).…”

Section: Resultsmentioning

confidence: 99%

Reconstruction and Analysis of the Transcriptome Regulatory Network of Clostridium Botulinum Type A3 Str. Loch Maree

ROJA B.,

THAMANNA L.,

CHELLAPANDI P.

2023

AJMBES

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Clostridium botulinum type A3 str. Loch Maree is a clinically important strain that produces botulinum neurotoxin type A3 and causes foodborne, infant, and wound botulism worldwide. Studying the mechanism underlying the virulence of this organism is imperative to understand its antibacterial resistance and discovering new drugs or inhibitors. The biochemical and molecular characteristics of this organism have been intensively studied, but their gene regulatory mechanisms are unclear. Hence, we reconstructed the transcriptional regulatory network from the complete genome of this organism and analyzed interactive genes from the identified hub module using a knowledge-based bottom-up approach. The biological reliability, topological properties, and robustness of the regulatory network model were validated with network parameters, followed by gene ontology terms and literature support. The reconstructed regulatory network consisted of 12 transcriptional regulators associated with 2369 coding genes. ResD, SpoOA, ComK, CcpC, DinR, DegU, CitT, CodY, GerE, GltC, GltR, IolR, and LevR were identified as transcriptional regulators from this organism homologous to Bacillus subtilis 168. These regulators have been shown to control beta-lactamase, methyl-accepting chemotaxis protein, DNA replication protein DnaD, sensor histidine kinase, and putative membrane proteins of this organism. This study also predicted all possible promoter sites in regulated genes and their associated molecular functions. We conclude that a global regulatory network model of this organism provides insights into its growth physiology and virulence elicitation in the human intestinal environment.

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Section: Resultsmentioning

confidence: 99%

Reconstruction and Analysis of the Transcriptome Regulatory Network of Clostridium Botulinum Type A3 Str. Loch Maree

ROJA B.,

THAMANNA L.,

CHELLAPANDI P.

2023

AJMBES

View full text Add to dashboard Cite

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“…Thus, a quick, cost-effective and safe recycling procedure is essential in order to reduce the risk of food-borne diseases in connecting with farms. Antitoxin administration and supportive therapies are currently used to treat veterinary animals and broilers (Prisilla and Chellapandi, 2019). The preventive measures of these organisms can increase the efficient production of livestock and poultry products, which in turn in resolving the problems of international trade.…”

Section: Resultsmentioning

confidence: 99%

A human–food web–animal interface on the prevalence of food-borne pathogens (Clostridia and Enterococcus) in mixed veterinary farms

Prisilla

Remin

Roja

et al. 2019

Food Sci Biotechnol

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In the present work, we addressed the impact of a human-food web-animal interface on the prevalence of food-borne pathogens in mixed farms of Tamil Nadu, India. We have isolated and identified six strains of Clostridium sp. and five strains of Enterococcus sp. from food and animal sources disposed near to the veterinary and poultry farms. Phylogenetic relationships of these strains were inferred from their homologies in 16S rDNA sequences and rRNA secondary structures. The strain PCP07 was taxonomically equivalent to C. botulinum confirmed by neurotoxin-specific PCR primers, followed by mouse bioassay. Other Clostridial and Enterococcal isolates have shown a phylogenetic similarity to the C. bifermentans and E. durans isolated from veterinary farms, respectively. Results of our study revealed that a humanfood web-animal interface has influenced the disease incidence and prevalence of these isolates in the poultry to veterinary farms, where human food acted as a likely transmittance vehicle for their infections.

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“…Electrophoretic mobility shift assay (EMSA) experiments were performed as described previously . The recombinant GntR10 protein was purified as described previously . The specific hot probes covering the GnrR10 binding sites were synthesized and labeled with biotin at the 3′ end.…”

Section: Methodsmentioning

confidence: 99%

ChIP‐seq analysis of Brucella reveals transcriptional regulation of GntR

Zhang

et al. 2019

J Basic Microbiol

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Transcriptional regulator GntR controls diverse physiological functions necessary for Brucella survival. In the intracellular pathogen Brucella, GntR has been shown to regulate the expression of genes related to virulence. However, the precise determination of GntR direct targets has so far proved elusive. Therefore, we performed chromatin immunoprecipitation of GntR10 followed by next-generation sequencing (ChIP-seq). We selected target gene BAB1_1163 directly regulated by GntR10 and created the mutant (2308Δ1163) from virulent Brucella abortus 2308 (S2308). 2308Δ1163 strain survival capability in murine macrophages (RAW 264.7) was detected and the levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) were also measured. We detected 88 intergenic ChIP-seq peaks of GntR10 binding distributed across the Brucella genome and determined a markedly asymmetric binding consensus motif with 12 bp length. 2308Δ1163 showed reduced survival capability in RAW 264.7. After the macrophages were infected with 2308Δ1163, the levels of TNF-α and IL-1β were decreased and were significantly lower than that for the S2308-infected group, indicating that the 2308Δ1163 mutant could inhibit the secretion of inflammatory cytokines. Taken together, the research has recorded valuable data about GntR10 and provided new insights into the functionality of GntR10. K E Y W O R D S2308Δ1163, Brucella abortus, ChIP-seq, GntR10 *Zhiqiang Li and Min Li contributed equally to this study.

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Cloning and expression of immunogenic Clostridium botulinum C2I mutant proteins designed from their evolutionary imprints

Cited by 4 publications

References 48 publications

Reconstruction and Analysis of the Transcriptome Regulatory Network of Clostridium Botulinum Type A3 Str. Loch Maree

Reconstruction and Analysis of the Transcriptome Regulatory Network of Clostridium Botulinum Type A3 Str. Loch Maree

A human–food web–animal interface on the prevalence of food-borne pathogens (Clostridia and Enterococcus) in mixed veterinary farms

ChIP‐seq analysis of Brucella reveals transcriptional regulation of GntR

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