Cloning and expression of murine CD40 ligand gene

Jiang, Yongfang; Su, Xian-shi; Gong, Guozhong; He, Yan; Xu, Yanyan

doi:10.11569/wcjd.v12.i6.1349

Cited by 1 publication

(2 citation statements)

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“…The expression vector containing murine CD40L cDNA encoding the entire coding region (pcDNA3.1 + -mCD40L) was produced and transfected into H22 cells as previously reported (Jiang et al, 2005a;2005b). In brief, transfections were performed by the lipofection method with Lipofectace reagent.…”

Section: Cdna and Transfectionmentioning

confidence: 99%

“…The expression murine CD40L (mCD40L) messenger ribonucleic acid (mRNA) of tumor tissue was treated either with liposome-pcDNA3.1 + -mCD40L complexes or control vector pcDNA3.1 + as previously reported (Jiang et al, 2005a;2005b). In brief, 100 mg of tumor liver tissues were homogenized by using a Mini-Beadbeater, and total RNA was isolated.…”

Section: Detection Of Mcd40l Expression By Immunohistochemistry and Rmentioning

confidence: 99%

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Cationic liposome-mediated transfection of CD40 ligand gene inhibits hepatic tumor growth of hepatocellular carcinoma in mice

Jiang

et al. 2009

J. Zhejiang Univ. Sci. B

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The mice were monitored for tumor growth weekly. We examined mCD40L messenger ribonucleic acid (mRNA) expression by reverse transcription polymerase chain reaction (RT-PCR) and the histologic changes in tumors at two weeks after intratumoral injection using immunohistochemical staining of tumor tissues. Results: All mice inoculated with parental H22 cells developed a tumor subcutaneously, and the tumor size increased progressively within three weeks. However, the mice receiving H22-CD40L cells exhibited complete regression of the tumor two weeks after tumor cell inoculation. The tumor-bearing animals with the treatment of pcDNA3.1 + or PBS, or without treatment had progressive tumor growth, while those mice treated with pcDNA3

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Section: Cdna and Transfectionmentioning

confidence: 99%

Section: Detection Of Mcd40l Expression By Immunohistochemistry and Rmentioning

confidence: 99%