Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System

Mohajeri, Abbas; Pilehvar-Soltanahmadi, Younes; Pourhassan‐Moghaddam, Mohammad; Abdolalizadeh, Jalal; Karimi, Pouran; Zarghami, Nosratollah

doi:10.15171/apb.2016.026

Cited by 11 publications

(10 citation statements)

References 33 publications

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“…However, there are important challenges in the clinical application of endostatin related to the chemical nature of this protein. These challenges include the short half-life and instability of the protein in vivo [106], the requirement of administering high volumes of endostatin to exert their anti-angiogenic effects, which in itself is associated with significant practical and cost implications [107], as well as technological challenges related to manufacturing a correctly folded and soluble protein to ensure adequate bioactivity within the tumour cells [108]. To overcome these challenges, nanotechnology has been utilized to manufacture nanoparticles as transporters of this protein [109].…”

Section: Novel and Future Approaches To Modify Angiogenesis As Anti-cancer Optionmentioning

confidence: 99%

Inducing Angiogenesis, a Key Step in Cancer Vascularization, and Treatment Approaches

Saman

Raza²,

Uddin

et al. 2020

Cancers

119

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Angiogenesis is a term that describes the formation of new blood and lymphatic vessels from a pre-existing vasculature. This allows tumour cells to acquire sustenance in the form of nutrients and oxygen and the ability to evacuate metabolic waste. As one of the hallmarks of cancer, angiogenesis has been studied extensively in animal and human models to enable better understanding of cancer biology and the development of new anti-cancer treatments. Angiogenesis plays a crucial role in the process of tumour genesis, because solid tumour need a blood supply if they are to grow beyond a few millimeters in size. On the other hand, there is growing evidence that some solid tumour exploit existing normal blood supply and do not require a new vessel formation to grow and to undergo metastasis. This review of the literature will present the current understanding of this intricate process and the latest advances in the use of angiogenesis-targeting therapies in the fight against cancer.

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Section: Novel and Future Approaches To Modify Angiogenesis As Anti-cancer Optionmentioning

confidence: 99%

Inducing Angiogenesis, a Key Step in Cancer Vascularization, and Treatment Approaches

Saman

Raza²,

Uddin

et al. 2020

Cancers

119

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“…First, tumor treatment needs to large quantities of biologically active Es and this, in turn, increases the importance of production of bioactive rEs in large quantities which, is difficult and has high cost. 10 , 46 - 48 Second, protein production system may disable in producing soluble protein and correct folding of protein which affects its bioactivity. 49 Third, the endostatin-purification process may denature its structure and the resultant yields may be low.…”

Section: Clinical Application Challengesmentioning

confidence: 99%

The Challenges of Recombinant Endostatin in Clinical Application: Focus on the Different Expression Systems and Molecular Bioengineering

Mohajeri¹,

Sanaei

Kiafar³

et al. 2017

Adv Pharm Bull

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Angiogenesis plays an essential role in rapid growing and metastasis of the tumors. Inhibition of angiogenesis is a putative strategy for cancer therapy. Endostatin (Es) is an attractive anti-angiogenesis protein with some clinical application challenges including; short half-life, instability in serum and requirement to high dosage. Therefore, production of recombinant endostatin (rEs) is necessary in large scale. The production of rEs is difficult because of its structural properties and is high-cost. Therefore, this review focused on the different expression systems that involved in rEs production including; mammalian, baculovirus, yeast, and Escherichia coli (E. coli) expression systems. The evaluating of the results of different expression systems declared that none of the mentioned systems can be considered to be generally superior to the other. Meanwhile with considering the advantages and disadvantage of E. coli expression system compared with other systems beside the molecular properties of Es, E. coli expression system can be a preferred expression system for expressing of the Es in large scale. Also, the molecular bioengineering and sustained release formulations that lead to improving of its stability and bioactivity will be discussed. Point mutation (P125A) of Es, addition of RGD moiety or an additional zinc biding site to N-terminal of Es , fusing of Es to anti-HER2 IgG or heavy-chain of IgG, and finally loading of the endostar by PLGA and PEG- PLGA nanoparticles and gold nano-shell particles are the effective bioengineering methods to overcome to clinical changes of endostatin.

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“…Codon usage has been extensively used to assess an organism based on its genetically expressions (Mohajeri et al 2016) and including comparisons among vertebrates (Kattoor et al 2015) and mammals (Jørgensen et al 2005). In this study (Table 1), bat populations from east Kalimantan were identified having the lowest codon frequencies and in contrast the central Sulawesi has the highest codon frequency among others.…”

Section: Resultsmentioning

confidence: 99%

Phylogeography, codon usages, and DNA barcoding of lesser short nosed fruit bat (Cynopterus brachyotisMuller, 1838) populations in Indonesia

Wibowo

2021

Preprint

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Fruit bat (Pteropodidae) is one of the mammals that is common in environments and widely distributed from subtropical to tropical Asia. Whereas the information on phylogeography of fruit bat Cynopterus brachyotis is still limited. From this situation, this paper aims to assess the phylogeography, codon usage, and DNA barcoding of C. brachyotis populations in Indonesia. Phylogeography was developed based on 657 bp of the mtDNA COI gene for all bat individuals and Bayesian inference to construct the phylogeny tree. The C. brachyotis populations in Indonesia are different to the populations from the Asia continent. The results show that C. brachyotis populations in Indonesia were divided into 3 distinct clades. A putative geographical barrier, recent, and rapid range expansion in the Sunda lineage associated with changes in sea levels, possibly coupled with related ecological differences, may have driven population divergence, allopatric, and sympatric speciations. Codon usage and high frequency were also contributing to the dispersal of C. brachyotis forming a distinct population.

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Cloning and Expression of Recombinant Human Endostatin in Periplasm of Escherichia coli Expression System

Cited by 11 publications

References 33 publications

Inducing Angiogenesis, a Key Step in Cancer Vascularization, and Treatment Approaches

Inducing Angiogenesis, a Key Step in Cancer Vascularization, and Treatment Approaches

The Challenges of Recombinant Endostatin in Clinical Application: Focus on the Different Expression Systems and Molecular Bioengineering

Phylogeography, codon usages, and DNA barcoding of lesser short nosed fruit bat (Cynopterus brachyotisMuller, 1838) populations in Indonesia

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