Cloning and Expression of Serratia Marcescens Prodigiosin Gene in Ecoli XL1blue

Abstract

Background and Objectives: Serratia is a gram-negative bacterium. The pigmentation property of Serratia Marcescens is used as a marker of dust particles in the environment and in the hospital. Today biopigments are also widely used in the manufacture and production of pharmaceutical products. Prodigiosin is a promising drug due to its reported properties of antifungal immunosuppressive and anti-proliferative activities. In the present study, cloning of pig gene- isolated from Serratia Marcescens in Ecoli XL1blue was performed. Subjects and Methods 60 Samples were taken from clinical sources of patients hospitalized with urinary tract infections in Saveh Hospitals. Serratia Marcescens were identified and isolated by different tests. The pig gene was cloned by T-A cloning using PTG-19 vector into the Escherichia coli XL1blue as host. Expression of cloned gene in recombinant colonies was evaluated by Real time PCR. The phylogenetic tree was plotted using clustalX and Mega5 software Results Screening of samples identified 12 isolates of Serratia Marcescens from then 4 isolates had pig gene. Expression of Pig gene in Escherichia coli XL1blue was confirmed by Real-Tima PCR. As a result of phylogenetic studies, some close relatives of serratia have been identified as candidates for further studies Conclusion Serratia Marcescens can be considered as a rich source of pigments with many applications and can be used as indigenous strains to produce Prodigiosin.