Cloning and expression of the cDNA encoding rabbit liver carbonyl reductase

Gonzalez, B.; Sapra, Amita; Rivera, Hector; Kaplan, William D.; Yam, Brian; Forrest, Gerald L.

doi:10.1016/0378-1119(94)00843-h

Cited by 23 publications

(10 citation statements)

References 5 publications

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“…The role of these genes in DS phenotypes is unknown: (i) Carbonyl reductase (CBR) is one of several monomeric, NADPH-dependent oxidoreductases with wide specificity for carbonyl compounds. Lymphoblasts with an increased copy number of CBR show increased aldo-, keto-, and quinone reductase activity (Gonzalez et al, 1995). (ii) SIM2 is a homologue of the Drosophila single-minded gene.…”

Section: Down Syndrome Critical Region (Dscr) On Hc21mentioning

confidence: 99%

10 Years ofGenomics,Chromosome 21, and Down Syndrome

Antonarakis

1998

Genomics

142

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Section: Down Syndrome Critical Region (Dscr) On Hc21mentioning

confidence: 99%

10 Years ofGenomics,Chromosome 21, and Down Syndrome

Antonarakis

1998

Genomics

142

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“…5). RT-PCR was carried out using the primers [40]; hCR, human carbonyl reductase [16]; pig 20bHSD, pig 20b-hydroxysteroid dehydrogenase [5]; rab CR, rabbit carbonyl reductase [46]; rat CR, rat carbonyl reductase [37]; mouse CR, mouse carbonyl reductase 2 [38]. Open squares indicate NADP 1 -binding (TGxxxGxG) and SDR family active site motifs (S-Y-K), respectively.…”

Section: Distribution Of Cr In Chinese Hamster Tissuesmentioning

confidence: 99%

Cloning and bacterial expression of monomeric short-chain dehydrogenase/reductase (carbonyl reductase) from CHO-K1 cells

Terada¹,

Sugihara²,

Nakamura³

et al. 2000

Eur J Biochem

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“…The presence of multiple forms of befunolol reductase in rabbit liver cytosol was also reported, although one form was identified as CBR (Imamura et al, 1989). However, in addition to the properties of the other multiple forms, the entire primary sequences of the enzymes other than CBR (Gonzalez et al, 1995) have not been studied, and the interrelationship among the enzymes remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Cloning and Characterization of Four Rabbit Aldo-Keto Reductases Featuring Broad Substrate Specificity for Xenobiotic and Endogenous Carbonyl Compounds: Relationship with Multiple Forms of Drug Ketone Reductases

et al. 2014

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Multiple forms of reductases for several drug ketones were isolated from rabbit liver, but their interrelationship and physiologic roles remain unknown. We isolated cDNAs for four aldo-keto reductases (AKR1C30, AKR1C31, AKR1C32, and AKR1C33), which share high amino acid sequence identity with the partial sequences of two rabbit naloxone reductases. The four recombinant enzymes reduced a variety of carbonyl compounds, including endogenous a-dicarbonyls (e.g., isatin and diacetyl), aldehydes (e.g., farnesal and 4-oxo-2-nonenal), and ketosteroids. They differed in specificity for drug ketones and ketosteroids. Although daunorubicin and befunolol were common substrates of all of the enzymes, AKR enzymes specifically reduced naloxone (AKR1C30, AKR1C32, and AKR1C33), metyrapone (AKR1C32 and AKR1C33), loxoprofen (AKR1C31 and AKR1C32), ketotifen (AKR1C30), and naltrexone and fenofibric acid (AKR1C33). AKR1C30 reduced only 17-keto-5b-androstanes, whereas the other enzymes were active toward 3-, 17-, and 20-ketosteroids, and AKR1C33 further reduced 3-keto groups of bile acids and 7a-hydroxy-5b-cholestanes. In addition, AKR1C30, AKR1C31, AKR1C32, and AKR1C33 were selectively inhibited by carbenoxolone, baccharin, phenolphthalein, and zearalenone, respectively. The mRNAs for the four enzymes were ubiquitously expressed in male rabbit tissues, in which highly expressed tissues were the brain, heart, liver, kidney, intestine, colon, and testis (for AKR1C30 and AKR1C31); brain, heart, liver, kidney, testis, lung, and adrenal gland (for AKR1C32); and liver and intestine (for AKR1C33). Thus, the four enzymes correspond to the multiple drug ketone reductases, and may function in the metabolisms of steroids, isatin and reactive carbonyl compounds, and bile acid synthesis.

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Cloning and expression of the cDNA encoding rabbit liver carbonyl reductase

Cited by 23 publications

References 5 publications

10 Years ofGenomics,Chromosome 21, and Down Syndrome

10 Years ofGenomics,Chromosome 21, and Down Syndrome

Cloning and bacterial expression of monomeric short-chain dehydrogenase/reductase (carbonyl reductase) from CHO-K1 cells

Cloning and Characterization of Four Rabbit Aldo-Keto Reductases Featuring Broad Substrate Specificity for Xenobiotic and Endogenous Carbonyl Compounds: Relationship with Multiple Forms of Drug Ketone Reductases

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