Cloning and Expression of the Isocitrate Lyase Gene from a Nitrogen-Fixing Bacterium,<i>Azotobacter vinelandii</i>, and Functional Analysis of the Enzyme by Site-Directed Mutagenesis

Tanaka, Yumi; Hayashi, Tomofumi; Yamaoka, Naoto; Takada, Yasuhiro

doi:10.1271/bbb.110518

Cited by 2 publications

(1 citation statement)

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“…In addition, the indicators light intensities changes during the formation of acidic metabolic products and pH changes (at pH 7.6, the bromothymol blue indicator is blue, and at pH 6.0, it is yellow). In the A. vinelandii growth and alginate production experiments, we used modified M22 medium 16 of the following composition (g/l): KH2PO4 -0.011; Na2НРО4 -0,189; MgSО4 7Н2O -0,2; CaSO4 2Н2О -0,02; FeSO4 7H2O -0,006; MoO3 -0,00005; NaCl -0.01; NaHCO3 -0.05, pH 7.8. Carbon sources (sucrose, glucose, lactose, and mannitol) were added at concentrations 1.5; 2; 4%.…”

Section: Strain and The Culture Mediamentioning

confidence: 99%

Effect of Nutrient Sources on the Alginate Accumulation in the Culture Liquid of Azotobacter vinelandii D-05 and Obtaining Biocomposite Materials

Ревин

Kostina

Revina

et al. 2018

Braz. arch. biol. technol.

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Using the classic biotechnological methods, the dependence of A. vinelandii D-05 culture alginate production from the media carbon and nitrogen content was investigated. The maximal alginate production was observed during cultivation bacterium in the medium with 2 to 4% of sucrose, but the maximal growth was found in the medium with 4% glucose. It was found that for the alginate production the optimal nitrogen contents could take from 0.05% yeast extract (carbon: nitrogen ratio 168:1). For the first time we demonstrated possibility the A. vinelandii growth during the cultivation in a medium with molasses (a by-product of sugar production) and the significant polysaccharide production (16.6 g/l) was obtained. It was established, that A. vinelandii culture broth could be used as a biological binder for obtaining the biocomposite materials.

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Section: Strain and The Culture Mediamentioning

confidence: 99%